茶树WAT1基因家族鉴定及胁迫响应分析

何岸; 曹孟飞; 周瑾; 王玉春; 张可欣; 陈雅楠

doi:10.13305/j.cnki.jts.2026.05.002

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茶叶科学 ›› 2026, Vol. 46 ›› Issue (5) : 807-820. DOI: 10.13305/j.cnki.jts.2026.05.002

研究报告

茶树WAT1基因家族鉴定及胁迫响应分析

何岸¹, 曹孟飞¹, 周瑾¹, 王玉春¹, 张可欣^2,*, 陈雅楠^1,*

作者信息 +

Genome-wide Identification and Stress Response Analysis of the WAT1 Gene Family in Camellia sinensis

HE An¹, CAO Mengfei¹, ZHOU Jin¹, WANG Yuchun¹, ZHANG Kexin^2,*, CHEN Yanan^1,*

Author information +

文章历史 +

摘要

茶树（Camellia sinensis）是我国重要的经济作物之一,其生长发育、产量及品质经常受到炭疽病等真菌性病害的严重威胁。植物抗病反应涉及多种跨膜转运蛋白及复杂激素信号调控网络的协同作用。WAT1（Walls are thin 1）基因家族编码植物特异性膜转运蛋白,在生长素运输、次生细胞壁形成以及逆境胁迫响应等生物学过程中发挥重要作用。本研究基于茶树全基因组数据,对茶树WAT1基因（CsWAT1）家族成员进行了全基因组鉴定,并系统分析了其编码蛋白的理化性质、系统发育关系、染色体分布、基因结构、启动子顺式作用元件组成以及组织表达特征。结果表明,茶树基因组中共鉴定获得41个CsWAT1成员,其编码蛋白的分子量介于15.21~48.15 kDa,均预测含有多个跨膜螺旋。亚细胞定位预测结果显示,32个CsWAT1定位于质膜,9个CsWAT1定位于液泡膜,且大部分成员均表现为碱性蛋白特征。系统发育分析表明,CsWAT1家族可划分为8个亚族,且与拟南芥WAT1家族成员之间存在广泛的共线性关系。启动子顺式作用元件分析发现,CsWAT1启动子区域富含光响应、植物激素响应以及非生物和生物胁迫响应相关元件。组织表达分析显示,CsWAT1家族成员在不同组织中呈现差异化表达模式。进一步利用RT-qPCR分析发现,CsWAT1-11在山茶炭疽菌（Colletotrichum camelliae）侵染及茉莉酸甲酯（MJ）处理条件下均受到显著诱导表达。本研究系统鉴定并分析了CsWAT1家族的成员特征、进化关系及表达模式,揭示了部分CsWAT1在病原菌胁迫响应中的潜在功能,为后续解析CsWAT1在茶树炭疽病响应中的功能提供候选基因和理论参考。

Abstract

Tea plants (Camellia sinensis) are one of the important economic crops in China. The growth, development, yield and quality of tea plants are severely threatened by fungal diseases such as anthracnose. Plant disease resistance involves complex hormone signaling regulatory networks and the coordinated action of various transmembrane transport proteins. The WAT1 (Walls are thin 1) gene family encodes plant-specific membrane transport proteins that play important roles in auxin transport, secondary cell wall formation and stress responses. In this study, using tea plant genome data, we performed a genome-wide identification of the WAT1 gene family in Camellia sinensis (CsWAT1) and systematically analyzed the physicochemical properties, phylogenetic relationships, chromosomal distribution, gene structure, promoter cis-acting element composition, and tissue expression profiles of the encoded proteins. A total of 41 CsWAT1 gene members were identified in the tea genome, with molecular weights ranging from 15.21 kDa to 48.15 kDa, and all proteins were predicted to contain multiple transmembrane helices. Subcellular localization prediction reveals that 32 CsWAT1 proteins are localized to the plasma membrane and 9 to the vacuolar membrane, with almost all members displaying basic characteristics. Phylogenetic analysis indicates that the CsWAT1 family can be divided into 8 subgroups, and extensive syntenic relationships were identified between CsWAT1 members and those of Arabidopsis thaliana. Promoter cis-element analysis reveals that the promoter regions of CsWAT1 genes are enriched for elements related to light response, phytohormone response, and abiotic and biotic stress responses. Tissue expression analysis shows that CsWAT1 family members exhibit distinct expression patterns across different organs. Further RT-qPCR analysis reveals that CsWAT1-11 was significantly induced under both Colletotrichum camelliae infection and methyl jasminate (MJ) treatment. This study represents the first systematic identification and analysis of the CsWAT1 gene family in tea plants, encompassing member characteristics, evolutionary relationships, and expression profiles. These findings reveal the potential roles of certain CsWAT1 genes in pathogen responses and provide candidate genes and a theoretical basis for further elucidating the functions of CsWAT1 in tea plant responses to anthracnose.

导出引用

何岸, 曹孟飞, 周瑾, 王玉春, 张可欣, 陈雅楠. 茶树WAT1基因家族鉴定及胁迫响应分析[J]. 茶叶科学. 2026, 46(5): 807-820 https://doi.org/10.13305/j.cnki.jts.2026.05.002

HE An, CAO Mengfei, ZHOU Jin, WANG Yuchun, ZHANG Kexin, CHEN Yanan. Genome-wide Identification and Stress Response Analysis of the WAT1 Gene Family in Camellia sinensis[J]. Journal of Tea Science. 2026, 46(5): 807-820 https://doi.org/10.13305/j.cnki.jts.2026.05.002

中图分类号： S571.1 Q52

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