CsCYP75B1响应低温胁迫调控茶树黄酮醇代谢的分子机制

王晓东; 李子俊; 王漪; 孙平; 韩晓阳; 范延艮; 黄晓琴

doi:10.13305/j.cnki.jts.2026.05.009

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茶叶科学 ›› 2026, Vol. 46 ›› Issue (5) : 873-890. DOI: 10.13305/j.cnki.jts.2026.05.009

研究报告

CsCYP75B1响应低温胁迫调控茶树黄酮醇代谢的分子机制

王晓东¹, 李子俊¹, 王漪², 孙平¹, 韩晓阳¹, 范延艮¹, 黄晓琴^1,*

作者信息 +

The Molecular Mechanism of CsCYP75B1 in Response to Low-temperature Stress and Its Regulation of Flavonol Metabolism in Tea Plants

WANG Xiaodong¹, LI Zijun¹, WANG Yi², SUN Ping¹, HAN Xiaoyang¹, FAN Yangen¹, HUANG Xiaoqin^1,*

Author information +

文章历史 +

摘要

茶树是我国重要的经济作物,生长易受温度影响,低温胁迫严重限制其产量与品质。黄酮醇作为植物次生代谢产物,已被证实参与非生物胁迫响应。黄酮醇的代谢受多种基因的网络调控。CsCYP75B1（CSS0048905）为课题组前期基于转录组测序数据筛选得到,是低温下黄酮醇生物合成途径中显著差异表达的基因。为探究CsCYP75B1在低温下调控茶树黄酮醇代谢的分子机制,本研究运用生物信息学方法对其进行分析,并通过异源转化拟南芥和茶树瞬时沉默试验验证其功能。结果表明,CsCYP75B1的启动子区含有低温响应元件LTR,低温下CsCYP75B1表达上调,进而促进黄酮醇积累。在拟南芥中过表达CsCYP75B1可显著增强植株耐寒性,提高抗氧化酶活性,降低膜脂过氧化程度,并正向调控ICE-CBF-COR通路关键基因的表达。在茶树中瞬时沉默CsCYP75B1,叶片黄酮醇合成减少、抗氧化能力下降。本研究揭示了低温胁迫下茶树中CsCYP75B1通过正调控黄酮醇合成与低温信号通路,共同提升茶树抗寒性的分子机制,为茶树抗逆育种提供了候选基因,同时为茶树抗逆品质形成提供了依据。

Abstract

Tea plant is an important economic crop in China, and its growth is easily affected by temperature. Low-temperature stress severely limits its yield and quality. Flavonols, as secondary metabolites of plants, have been proven to be involved in abiotic stress responses. The metabolism of flavonols is regulated by a network of multiple genes. Our research team previously screened transcriptome sequencing data and identified a significantly differentially expressed gene, CsCYP75B1 (CSS0048905), in the flavonol biosynthesis pathway under low temperature. CsCYP75B1 belongs to the cytochrome P450 family and encodes a F3'H enzyme, which is a key enzyme in the flavonoid biosynthesis. The function of CsCYP75B1 in tea plant response to low temperature is unclear. To explore the molecular mechanism of CsCYP75B1 in regulating flavonol metabolism in tea plants under low temperature, this experiment analyzed the cloned CsCYP75B1 using bioinformatics methods and verified its function through heterologous transformation of Arabidopsis thaliana and transient silencing experiments in tea plants. The results show that the promoter region of the CsCYP75B1 gene contains a low temperature response element (LTR), and the expression of CsCYP75B1 was upregulated under low temperature, thereby promoting flavonol accumulation. Overexpressing CsCYP75B1 in Arabidopsis thaliana significantly enhanced plant cold tolerance, increased antioxidant enzyme activity, reduced membrane lipid peroxidation, and positively regulated the expression of key genes in the ICE-CBF-COR pathway. Transient silencing of the CsCYP75B1 gene in tea plants resulted in reduced flavonol synthesis in leaves and decreased antioxidant capacity. This study reveals the molecular mechanism by which the CsCYP75B1 gene in tea plants positively regulates flavonol synthesis and the low temperature signaling pathway under low-temperature stress, thereby enhancing their cold tolerance. It provided candidate genes for breeding stress-resistant tea cultivars and relevant evidence for the formation of stress-resistant quality in tea plants.

导出引用

王晓东, 李子俊, 王漪, 孙平, 韩晓阳, 范延艮, 黄晓琴. CsCYP75B1响应低温胁迫调控茶树黄酮醇代谢的分子机制[J]. 茶叶科学. 2026, 46(5): 873-890 https://doi.org/10.13305/j.cnki.jts.2026.05.009

WANG Xiaodong, LI Zijun, WANG Yi, SUN Ping, HAN Xiaoyang, FAN Yangen, HUANG Xiaoqin. The Molecular Mechanism of CsCYP75B1 in Response to Low-temperature Stress and Its Regulation of Flavonol Metabolism in Tea Plants[J]. Journal of Tea Science. 2026, 46(5): 873-890 https://doi.org/10.13305/j.cnki.jts.2026.05.009

中图分类号： S571.1 S330 Q52

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