以Caco-2细胞为模型,对比分析了一种黑茶不同提取物中氟的吸收转运及生物有效性。结果表明,黑茶不同提取物组分中氟含量差异明显,粗茶多糖组分（RTP）氟含量最高,是水提取物（TE）氟含量的2.17倍,RTP经水透析后得到的透析多糖（DTP）氟含量则显著降低,仅为RTP氟含量的1/22,TE氟含量的1/10。来自3种茶提取物中的氟以及氟化钠（NaF）中的氟在Caco-2细胞模型中的正向和逆向转运都有时间和浓度剂量效应,随着时间的延长、浓度升高而显著增加;处理1 h后,NaF处理组细胞对氟正向和逆向转运量最高,DTP中氟的正向和逆向转运量最低,与其他各处理达到显著水平,TE和RTP处理间差异不明显。表观渗透系数（Papp）随处理时间延长呈下降趋势,其中,NaF中的氟的Papp在4 h各阶段都大于1×10-5 cm·s-1,说明其生物有效性良好,易于吸收;TE和RTP氟处理2 h后,Papp下降至小于1×10-5 cm·s-1,说明其生物有效性中等;DTP氟处理各阶段Papp都最小,在处理1 h后下降至小于1×10-5 cm·s-1,提示其吸收性最弱,生物有效性最差。NaF、TE以及DTP中的氟在所试浓度范围内,是以被动扩散为主,而RTP氟在较高浓度存在主动转运形式。由此可见,在小肠吸收模型中,黑茶提取物中的氟较NaF中的氟生物利用度低,而茶叶中氟的结合形式影响其生物有效性。
Abstract
In this study, absorption, transportation and bioavailability of fluorine in different extracts of a dark tea were evaluated in the Caco-2 cell line model. The results show that the fluorine contents in different extracts of dark tea were significantly different. The fluorine content of the crude polysaccharide fraction (RTP) was the highest, which was 2.17 times that of the water extract (TE). Fluorine content in dialyzed polysaccharide (DTP) was significantly reduced, which was only 1/22 of the fluorine content in RTP and 1/10 of the fluorine content in TE. The forward and reverse transport of fluorine in cell model had time and dose effects, and increased with the increase of time and concentration. After the first 1 h treatment, the forward and reverse transport of fluorine in NaF was the highest, while that in DTP was the lowest, which were significantly different from other treatments. There was no significant difference between the TE and RTP treatments. The apparent permeability coefficient (Papp) decreased with the prolonging of treatment time. The Papp of fluorine in NaF was more than 1×10-5 cm·s-1 within 4 h, indicating its bioavailability was good. The Papp of fluorine in TE and RTP decreased to less than 1×10-5 cm·s-1 after 2 h of treatment, indicating its bioavailability was moderate. the Papp of fluorine in DTP was the smallest at all stages, and was less than 1×10-5 cm·s-1 after 1 h of treatment, indicating its bioavailability was low. Fluorine in NaF, TE or DTP was mainly transported by passive diffusion within the tested concentration range, while fluorine in RTP had an active transport at a higher concentration. In conclusion, in the intestinal epitheliums model, the fluorine in the dark tea has lower bioavailability than that in NaF, and the binding form of the fluorine in the dark tea might affect its bioavailability.
关键词
Caco-2 /
氟化物 /
黑茶 /
生物有效性 /
转运
Key words
bioavailability /
Caco-2 /
dark tea /
fluoride /
transport
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