不同黄化茶树种质中咖啡碱合成部位的研究

张亚真; 钟思彤; 陈志辉; 孔祥瑞; 单睿阳; 郑士琴; 余文权; 陈常颂

doi:10.13305/j.cnki.jts.2024.04.011

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茶叶科学 ›› 2024, Vol. 44 ›› Issue (4) : 575-584. DOI: 10.13305/j.cnki.jts.2024.04.011

研究报告

不同黄化茶树种质中咖啡碱合成部位的研究

张亚真¹, 钟思彤¹, 陈志辉¹, 孔祥瑞¹, 单睿阳¹, 郑士琴¹, 余文权^2,*, 陈常颂^1,*

作者信息 +

Study on the Synthetic Site of Caffeine in Different Etiolated Tea Germplasms

ZHANG Yazhen¹, ZHONG Sitong¹, CHEN Zhihui¹, KONG Xiangrui¹, SHAN Ruiyang¹, ZHENG Shiqin¹, YU Wenquan^2,*, CHEN Changsong^1,*

Author information +

文章历史 +

摘要

咖啡碱作为茶树中的主要特征代谢物,是茶叶品质风味形成的重要组分和天然功能性成分。目前,茶树中咖啡碱的功能作用、分布规律、合成途径及关键基因已基本探明,但在亚细胞水平上,咖啡碱的合成部位有待进一步明确。以白鸡冠茶树及其自然杂交后代的不同黄化单株为材料,通过透射电镜对叶片细胞超微结构的观察,发现黄化叶片中叶绿体结构均有不同程度的受损,且与叶片SPAD值及叶色表型紧密相关;通过高效液相色谱法测定咖啡碱含量,发现黄化叶片中仍有大量咖啡碱积累,甚至超过正常绿色叶片。通过咖啡碱合成关键基因CsTCS1表达量测定、原位杂交及亚细胞定位发现,CsTCS1在不同黄化茶树种质叶片中的表达信号强度存在差异,但表达部位基本相同,主要分布在栅栏组织的细胞核和细胞质中;在亚细胞水平上,茶树叶片中咖啡碱的合成部位主要是细胞核和细胞质。

Abstract

As the main characteristic metabolite in tea plants, caffeine contributes to tea quality and flavor formation and is a natural functional component. Its function, distribution, biosynthetic pathway and related key genes in tea plants have been basically identified, but its synthetic site at subcellular level needs to be further clarified. In this study, ‘Baijiguan’ and its half-sib offsprings with different etiolated leaves were used as materials. The results of transmission electron microscopy show that the chloroplast structures in etiolated leaves were damaged or destroyed, which was closely related to the SPAD value and leaf phenotype. High performance liquid chromatography (HPLC) was used to determine the caffeine content. It was found that there was still a large amount of caffeine accumulation in etiolated leaves, even more than in normal green leaves. Then, the expression and location of CsTCS1, a key gene involved in caffeine synthesis, were studied by real-time PCR, in-situ hybridization and subcellular localization. It was shown that the expression level of CsTCS1 in different etiolated leaves varied obviously. But the expression site was basically consistent, mainly distributed in the nucleus and cytoplasm of palisade tissues. These results reveal that the synthetic site of caffeine at subcellular level in tea leaves were mainly nucleus and cytoplasm, but not chloroplasts.

导出引用

张亚真, 钟思彤, 陈志辉, 孔祥瑞, 单睿阳, 郑士琴, 余文权, 陈常颂. 不同黄化茶树种质中咖啡碱合成部位的研究[J]. 茶叶科学. 2024, 44(4): 575-584 https://doi.org/10.13305/j.cnki.jts.2024.04.011

ZHANG Yazhen, ZHONG Sitong, CHEN Zhihui, KONG Xiangrui, SHAN Ruiyang, ZHENG Shiqin, YU Wenquan, CHEN Changsong. Study on the Synthetic Site of Caffeine in Different Etiolated Tea Germplasms[J]. Journal of Tea Science. 2024, 44(4): 575-584 https://doi.org/10.13305/j.cnki.jts.2024.04.011

中图分类号： S571.1 Q52

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