研究茶多酚对慢性不可预知应激抑郁模型中小鼠的抗抑郁作用及机制。建立慢性不可预知性刺激小鼠抑郁模型,通过小鼠旷场实验、强迫游泳实验和悬尾实验中对茶多酚的抗抑郁作用进行研究;并采用分光光度法检测小鼠脑组织中丙二醛（MDA）的水平及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性,同时测定小鼠脑组织中5-羟色胺（5-hydroxytryptamine,5-HT）以及去甲肾上腺素（Norepinephrine,NE）的含量。给予茶多酚低高2个剂量组小鼠在旷场实验中的爬行格数与直立次数较模型组显著增加（#P<0.05,△P<0.001）;小鼠在强迫游泳实验和悬尾实验中的不动时间较模型组明显减少（#P<0.05,△P<0.001;#P<0.05,△P<0.05）,显示造模成功;此外,茶多酚可显著抑制脑组织中MDA的含量增加,并上调SOD和GSH-Px的活性,明显提升小鼠脑组织中5-HT和NE的含量。茶多酚对慢性不可预知应激引起的小鼠抑郁具有改善作用,其机制可能与上调脑部5-HT和NE的含量以及抑制脑组织氧化应激有关。
付小彬
,
缑灵山
,
何冬梅
,
贾根光
,
李岩
,
薛运生
,
李赛
,
蓝诺
,
刘毅
. 茶多酚改善慢性不可预知应激小鼠抑郁症状的作用[J]. 茶叶科学, 2013
, 33(4)
: 301
-305
.
DOI: 10.13305/j.cnki.jts.2013.04.014
The antidepressant effects of tea polyphenols on the chronic stress-induced depression in mice and the possible underlying mechanism were investigated in this paper. The antidepressant model of chronic unpredictable mild stress mice (CUMS) was established. The activities of the mice exposed to various stress were tested in the open-field, the immobility duration of the forced swimming test and tail suspension test were measured. Meanwhile, the contents of the 5-HT and the NE in the brain tissue were determined, and the activities of superoxide dismutase (SOD), GSH-Px and the content of malondialdehyde (MDA) in the brain tissue were estimated by spectrophotometry. The CUMS-induced depressive mice exhibited significant decrease in the activities in the open-field test as compared with the control group (#P<0.05, △P<0.001), while the immobility duration in the forced swimming test and tail suspension test were significantly increased, and the latency period (#P<0.05, △P<0.001. #P<0.05, △P<0.05) were shortened. However, tea polyphenols ameliorated the defecits of the behavior of the mice. Moreover, tea polyphenols increased the activities of SOD, GSH-Px, and the content of 5-HT, NE, and decreased the contents of MDA in the brain tissue. The depressive status can be attenuated by the administration of tea polyphenols, the underlying mechanism might be attributed to their antioxidative property and enhancement of serotonergic functions.
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