茶多酚改善慢性不可预知应激小鼠抑郁症状的作用

doi:10.13305/j.cnki.jts.2013.04.014

茶叶科学 ›› 2013, Vol. 33 ›› Issue (4): 301-305.doi: 10.13305/j.cnki.jts.2013.04.014

茶多酚改善慢性不可预知应激小鼠抑郁症状的作用

付小彬¹, 缑灵山¹, 何冬梅², 贾根光¹, 李岩¹, 薛运生¹, 李赛¹, 蓝诺¹, 刘毅^1,*

1. 徐州医学院药学院,江苏徐州 221004;
2. 中国水利水电科学研究院社区卫生服务站,北京 100038

收稿日期:2012-11-06 修回日期:2012-12-24 出版日期:2013-08-30 发布日期:2019-09-04
通讯作者: *cbpe201087@gmail.com
作者简介:付小彬(1987— ),男,山东聊城人,硕士,主要从事肿瘤及神经药理方面的研究。
基金资助:
江苏省“青蓝工程”资助; 江苏省六大人才高峰项目(2011-YY-13)、江苏高校优势学科建设工程资助项目、徐州市科技计划项目(XF11C037, XF11C062, XZZD1219, XZZD1227)、徐州医学院研究生科技创新计划项目（XYLC-1203）

The Antidepressant Effect of Tea Polyphenols to the Chronic Unpredictable Stress induced Depressive Mice

FU Xiao-bin¹, GOU Ling-shan¹, HE Dong-mei², JIA Gen-guang¹, LI Yan¹, XUE Yun-sheng¹, LI Sai¹, LAN Nuo¹, LIU Yi^1,*

1. Department of Pharmacy, Xuzhou Medical College, Xuzhou 221004, China;
2. Community Health Station, China Institute of Water Resources and Hydropower Research, Beijing 100038, China

Received:2012-11-06 Revised:2012-12-24 Online:2013-08-30 Published:2019-09-04

摘要/Abstract

摘要： 研究茶多酚对慢性不可预知应激抑郁模型中小鼠的抗抑郁作用及机制。建立慢性不可预知性刺激小鼠抑郁模型,通过小鼠旷场实验、强迫游泳实验和悬尾实验中对茶多酚的抗抑郁作用进行研究;并采用分光光度法检测小鼠脑组织中丙二醛（MDA）的水平及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性,同时测定小鼠脑组织中5-羟色胺（5-hydroxytryptamine,5-HT）以及去甲肾上腺素（Norepinephrine,NE）的含量。给予茶多酚低高2个剂量组小鼠在旷场实验中的爬行格数与直立次数较模型组显著增加（^#P<0.05,^△P<0.001）;小鼠在强迫游泳实验和悬尾实验中的不动时间较模型组明显减少（^#P<0.05,^△P<0.001;^#P<0.05,^△P<0.05）,显示造模成功;此外,茶多酚可显著抑制脑组织中MDA的含量增加,并上调SOD和GSH-Px的活性,明显提升小鼠脑组织中5-HT和NE的含量。茶多酚对慢性不可预知应激引起的小鼠抑郁具有改善作用,其机制可能与上调脑部5-HT和NE的含量以及抑制脑组织氧化应激有关。

关键词: 抑郁, 茶多酚, 慢性不可预知性刺激, 超氧化物歧化酶, 5-羟色胺

Abstract: The antidepressant effects of tea polyphenols on the chronic stress-induced depression in mice and the possible underlying mechanism were investigated in this paper. The antidepressant model of chronic unpredictable mild stress mice (CUMS) was established. The activities of the mice exposed to various stress were tested in the open-field, the immobility duration of the forced swimming test and tail suspension test were measured. Meanwhile, the contents of the 5-HT and the NE in the brain tissue were determined, and the activities of superoxide dismutase (SOD), GSH-Px and the content of malondialdehyde (MDA) in the brain tissue were estimated by spectrophotometry. The CUMS-induced depressive mice exhibited significant decrease in the activities in the open-field test as compared with the control group (^#P<0.05, ^△P<0.001), while the immobility duration in the forced swimming test and tail suspension test were significantly increased, and the latency period (^#P<0.05, ^△P<0.001. ^#P<0.05, ^△P<0.05) were shortened. However, tea polyphenols ameliorated the defecits of the behavior of the mice. Moreover, tea polyphenols increased the activities of SOD, GSH-Px, and the content of 5-HT, NE, and decreased the contents of MDA in the brain tissue. The depressive status can be attenuated by the administration of tea polyphenols, the underlying mechanism might be attributed to their antioxidative property and enhancement of serotonergic functions.

Key words: depression, tea polyphenols, CUMS, SOD, 5-hydroxytryptophan

中图分类号:

付小彬, 缑灵山, 何冬梅, 贾根光, 李岩, 薛运生, 李赛, 蓝诺, 刘毅. 茶多酚改善慢性不可预知应激小鼠抑郁症状的作用[J]. 茶叶科学, 2013, 33(4): 301-305. doi: 10.13305/j.cnki.jts.2013.04.014.

FU Xiao-bin, GOU Ling-shan, HE Dong-mei, JIA Gen-guang, LI Yan, XUE Yun-sheng, LI Sai, LAN Nuo, LIU Yi. The Antidepressant Effect of Tea Polyphenols to the Chronic Unpredictable Stress induced Depressive Mice[J]. Journal of Tea Science, 2013, 33(4): 301-305. doi: 10.13305/j.cnki.jts.2013.04.014.

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茶多酚改善慢性不可预知应激小鼠抑郁症状的作用

The Antidepressant Effect of Tea Polyphenols to the Chronic Unpredictable Stress induced Depressive Mice

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