茶叶不同提取物及不同茶叶对结核分枝杆菌抑制作用的研究

doi:10.13305/j.cnki.jts.2024.02.006

茶叶科学 ›› 2024, Vol. 44 ›› Issue (2): 341-349.doi: 10.13305/j.cnki.jts.2024.02.006

茶叶不同提取物及不同茶叶对结核分枝杆菌抑制作用的研究

许婧^1,2, 黄友谊³, 黄进⁴, 李春雷^2,*

1.平顶山职业技术学院旅游与酒店管理系,河南平顶山 467000;
2.潍坊科技学院农学院,山东省设施园艺生物工程研究中心,山东寿光 262700;
3.华中农业大学,园艺林学学院园艺植物生物学重点实验室,湖北武汉 430064;
4.十堰市果茶研究所,湖北十堰 442000

收稿日期:2023-11-14 修回日期:2024-01-29 出版日期:2024-04-15 发布日期:2024-04-30
通讯作者: * lcl432@163.com
作者简介:许婧,女,博士研究生,主要从事茶叶深加工及利用方面研究。
基金资助:
国家重点研发计划（2022YFD1600804）

Research on the Inhibition of Tea Extracts and Different Types of Tea on Mycobacterium tuberculosis

XU Jing^1,2, HUANG Youyi³, HUANG Jin⁴, LI Chunlei^2,*

1. Department of Tourism and Hospitality Management, Pingdingshan Vocational and Technical College, Pingdingshan 467000, China;
2. Shandong Province Facility Horticulture Bioengineering Research Center, College of Agriculture, Weifang Institute of Science and Technology, Shouguang 262700, China;
3. Tea Science Department of College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430064, China;
4. Shiyan Institute of Fruit and Tea, Shiyan 442000, China

Received:2023-11-14 Revised:2024-01-29 Online:2024-04-15 Published:2024-04-30

摘要/Abstract

摘要： 为探明茶叶对结核分枝杆菌H₃₇Ra菌株的抑制作用,采用牛津杯法对H₃₇Ra菌株进行抑菌试验,研究不同茶叶提取物（茶多酚、茶多糖和茶皂素）及不同种类茶叶（华农绿针、大宗炒青、广东大叶青、福鼎寿眉、铁观音、凤凰单丛、祁门红茶、下关沱茶、青砖茶）对H₃₇Ra菌株活性的抑制能力。结果显示,茶多酚对H₃₇Ra菌株表现出显著的抑菌能力,随着茶多酚浓度的增加,抑菌效果逐渐增强;在40 mg·mL^-1下,茶多酚显示出长期抑制H₃₇Ra菌株生长的能力;茶多糖和茶皂素对结核分枝杆菌没有抑制作用;不同种类茶叶表现出不同程度的抑菌能力,其中凤凰单丛的乙酸乙酯部较其他分离部有更强的抑菌能力,其60%乙醇洗脱的柱层析分离物抑菌能力最强,推测茶多酚是抑制H₃₇Ra菌株生长的主要成分。研究结果证实了茶叶对H₃₇Ra菌株具有抑制作用,且茶叶不同提取物和不同种类茶叶在抑菌能力上存在差异,为开发结核病相关的抗菌药物提供了一个新思路。

关键词: 茶叶提取物, 抑菌, 结核分枝杆菌H₃₇Ra菌株, 茶多酚

Abstract: This study was aimed at investigating the inhibitory effect of tea on Mycobacterium tuberculosis strain H₃₇Ra by using the Oxford cup method for the bacteriostatic assay of H₃₇Ra. The inhibitory abilities of different tea extracts (tea polyphenols, tea polysaccharides and tea saponins) and different teas (Huanong Lvzhen, Dazongchaoqing, Guangdong Dayeqing, Fuding Shoumei, Tieguanyin, Fenghuang Dancong, Keemun black tea, Xiaguan Tuocha and Green brick tea) on the activity of H₃₇Ra strain were studied. The results of the bacteriostatic experiments of tea extracts show that tea polyphenols exhibited significant bacteriostatic ability against H₃₇Ra strain and the bacteriostatic effect was gradually enhanced with the increase of tea polyphenol concentration. At a concentration of 40 mg·mL^-1, tea polyphenols show long-term inhibition of the growth of H₃₇Ra strain. Tea polysaccharides and saponins had no inhibitory effect on Mycobacterium tuberculosis. Different types of tea exhibit varying degrees of antibacterial ability, among which, the ethyl acetate fraction of Fenghuang Dancong had a stronger bacterial inhibition ability than other isolates. The column chromatography separated by 60% ethanol had the strongest bacterial inhibition ability, suggesting that tea polyphenols were the main components to inhibit the growth of H₃₇Ra strain. The results confirm that tea has an inhibitory effect on H₃₇Ra strain and different tea extracts and different tea types have different antibacterial abilities, which provides a new idea for the development of tuberculosis related antibacterial drugs.

Key words: tea extracts, bacteriostatic, Mycobacterium tuberculosis H₃₇Ra, tea polyphenols

中图分类号:

S571.1
R373

许婧, 黄友谊, 黄进, 李春雷. 茶叶不同提取物及不同茶叶对结核分枝杆菌抑制作用的研究[J]. 茶叶科学, 2024, 44(2): 341-349. doi: 10.13305/j.cnki.jts.2024.02.006.

XU Jing, HUANG Youyi, HUANG Jin, LI Chunlei. Research on the Inhibition of Tea Extracts and Different Types of Tea on Mycobacterium tuberculosis[J]. Journal of Tea Science, 2024, 44(2): 341-349. doi: 10.13305/j.cnki.jts.2024.02.006.

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茶叶不同提取物及不同茶叶对结核分枝杆菌抑制作用的研究

Research on the Inhibition of Tea Extracts and Different Types of Tea on Mycobacterium tuberculosis

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