茶鲜叶挥发物组分及对茶树病原菌的熏蒸抑制作用

doi:10.13305/j.cnki.jts.2021.03.005

摘要/Abstract

摘要： 为明确茶鲜叶中的挥发物组分及其与茶树主要病原菌之间的关系,利用HS-SPME-GC-MS法萃取分析茶鲜叶挥发物组分,并以茶褐枯病菌、茶云纹叶枯病菌、茶炭疽病菌、茶轮斑病菌为目标菌,测定茶鲜叶主要挥发物单体组分对病原菌的熏蒸抑制作用。结果显示,从茶鲜叶挥发物中分离到28个组分,鉴定出其中19个组分,占挥发物总量的94.405%,其主要组分为(Z)-己酸-3-己烯酯（18.395%）、乙酸叶醇酯（16.935%）、罗勒烯（12.615%）和顺-3-己烯基丁酯（11.210%）,其中顺-3-己烯基丁酯对4种病原菌均具有熏蒸抑制作用,EC₅₀均低于61.29 μL·L^-1。结果表明,顺-3-己烯基丁酯有作为熏蒸型杀菌剂应用于茶树病害防控的潜力。

关键词: 茶鲜叶, 熏蒸, 抑菌活性, 挥发物, 顺-3-己烯基丁酯

Abstract: In order to determine the relationship between volatile organic compounds (VOCs) from fresh tea leaves and the main fungal pathogens of tea plants, the VOCs from fresh tea leaves were extracted and analyzed by HS-SPME-GC-MS. The fumigation antifungal activities of volatile monomers from fresh tea leaves against four fungal pathogens including Botryosphaeria dothidea, Colletotrichum camelliae, Colletotrichum gloeosporioides, Pestalotiopsis theae were also studied. The results show that 28 chemical compounds were found from VOCs of fresh tea leaves, and 19 of them were characterized, accounting for 94.405% of the total VOCs. The main chemical compounds were hexanoic acid, 3-hexenyl ester, (Z)-hexanoic acid-3-hexenyl ester (18.395%), leaf acetate (16.935%), ocimene (12.615%), and cis-3-hexenyl butyrate (11.210%). Cis-3-hexenyl butyrate exhibited strong fumigation antifungal effect on the four tested fungal pathogens, and the EC₅₀ were lower than 61.29 μL·L^-1. In conclusion, cis-3-hexenyl butyrate can be used as a potential fumigant control of tea diseases.

Key words: fresh tea leaves, fumigation, antifungal activity, VOCs, cis-3-hexenyl butyrate

中图分类号:

智亚楠, 徐运飞, 朱明星, 王春生, 金银利, 陈利军. 茶鲜叶挥发物组分及对茶树病原菌的熏蒸抑制作用[J]. 茶叶科学, 2021, 41(3): 371-378. doi: 10.13305/j.cnki.jts.2021.03.005.

ZHI Yanan, XU Yunfei, ZHU Mingxing, WANG Chunsheng, JIN Yinli, CHEN Lijun. Volatile Organic Compounds from Fresh Tea Leaves and Their Fumigation Antifungal Activities on Fungal Pathogens of Tea Plants[J]. Journal of Tea Science, 2021, 41(3): 371-378. doi: 10.13305/j.cnki.jts.2021.03.005.

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