利用响应面分析法（Response Surface Analysis,RSM）对紫娟茶中花青素的提取工艺进行了优化。在单因素试验基础上,根据中心组合（Box-Behnken）试验设计原理和响应面分析法选取试验因素与水平,采用三因素三水平的响应面分析法对各个因素的显著性和交互作用进行分析,优化紫娟茶中花青素提取工艺。结果表明,紫娟茶中花青素提取的最佳工艺条件为：采用酸性甲醇为提取溶剂,提取温度29℃,提取时间132min,料液比1︰26;在此条件下浸提液中花青素的质量浓度可以达到5.94mg/mL。
Process extraction of anthocyanins from Zijuan tea was optimized by response surface methodology (RSM). Selected test factors and levels on the basis of the single-factor test, analysis of the significance of each factor and their interaction by using three factors and three levels of response surface analysis according to central composite (Box-Behnken) experimental design principles. The results showed that the optimum conditions for the extraction Zijuan tea anthocyanin are reaction temperature 29℃, reaction time 132min, the material-liquid ratio 1︰26 in acidic methanol, under these conditions the concentration of anthocyanins in the extract can be achieved 5.94mg/mL.
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