欢迎访问《茶叶科学》,今天是

茶叶科学 ›› 2012, Vol. 32 ›› Issue (3): 197-202.doi: 10.13305/j.cnki.jts.2012.03.002

• • 上一篇    下一篇

响应面法优化“紫娟”茶中花青素提取工艺的研究

费旭元1,2, 林智1*, 梁名志3, 王立波3, 陈继伟3, 吕海鹏1, 谭俊峰1, 郭丽1   

  1. 1. 中国农业科学院茶叶研究所,农业部茶及饮料植物产品加工与质量控制重点开放实验室,国家茶产业工程技术研究中心,浙江杭州 310008;
    2. 中国农业科学院研究生院,北京 100089;
    3. 云南农业科学院茶叶研究所,云南 勐海 666201
  • 收稿日期:2012-01-12 修回日期:2012-02-08 出版日期:2012-06-15 发布日期:2019-09-05
  • 通讯作者: linz@mail.tricaas.com
  • 作者简介:费旭元(1985— ),男,四川乐山人,硕士研究生,研究方向为茶叶加工与质量控制。
  • 基金资助:
    现代农业产业技术体系建设专项资金(CARS-23),农业部茶与饮料植物产品加工与质量控制重点开放实验室开放基金(2010K1001)

Study on Process of Anthocyanins Extraction from ‘Zijuan’ Tea by Response Surface Analysis

FEI Xu-yuan1,2, LIN Zhi1*, LIANG Ming-zhi3, WANG Li-bo3, CHEN Ji-wei3, LV Hai-peng1, TAN Jun-feng1, GUO Li1   

  1. 1. Tea Research Insititute, Chinese Academy of Agricultual Sciences, National Engineering Technology Research Center for Tea Industry; Key Laboratory of Processing and Quality Control of Tea and Beverage Plants, Ministry of Agriculture, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100089, China;
    3. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China
  • Received:2012-01-12 Revised:2012-02-08 Online:2012-06-15 Published:2019-09-05

摘要: 利用响应面分析法(Response Surface Analysis,RSM)对紫娟茶中花青素的提取工艺进行了优化。在单因素试验基础上,根据中心组合(Box-Behnken)试验设计原理和响应面分析法选取试验因素与水平,采用三因素三水平的响应面分析法对各个因素的显著性和交互作用进行分析,优化紫娟茶中花青素提取工艺。结果表明,紫娟茶中花青素提取的最佳工艺条件为:采用酸性甲醇为提取溶剂,提取温度29℃,提取时间132min,料液比1︰26;在此条件下浸提液中花青素的质量浓度可以达到5.94mg/mL。

关键词: 紫娟茶, 花青素, 提取, 响应面法

Abstract: Process extraction of anthocyanins from Zijuan tea was optimized by response surface methodology (RSM). Selected test factors and levels on the basis of the single-factor test, analysis of the significance of each factor and their interaction by using three factors and three levels of response surface analysis according to central composite (Box-Behnken) experimental design principles. The results showed that the optimum conditions for the extraction Zijuan tea anthocyanin are reaction temperature 29℃, reaction time 132min, the material-liquid ratio 1︰26 in acidic methanol, under these conditions the concentration of anthocyanins in the extract can be achieved 5.94mg/mL.

Key words: Zijuan tea, anthocyanins, extraction, response surface analysis

中图分类号: