建立了奶茶中三聚氰胺的HPLC测定方法。适用检测奶茶中三聚氰胺的HPLC条件为：色谱柱Sunfire C18柱（250 mm×4.6 mm ID: 5 μm）,流动相由0.008 mol/L辛烷磺酸钠和乙腈组成,柱温30℃,流速1.0 ml/min,检测波长236 nm,外标法定量。分析了市场上奶茶产品的三聚氰胺含量,发现少数奶茶产品中三聚氰胺的含量超过了国家暂行限量标准的2.5 mg/kg。
The method to determinate melamine in milk tea by HPLC, was established. The samples were analyzed by a Sunfire C18 column （250 mm×4.6 mm ID; 5 μm）. The mobile phase was composed of 0.008 mol/L sodium 1-Octanesulfonate （adjust to pH3.3 with acetic acid） and acetonitrile. The flow rate was 1.0 ml/min. Column temperature was 30℃. UV-detection was performed at 236 nm. The contents were quantified with exterior standard. Some milk tea samples from the supermarket were analyzed, and results showed that the content of melamine in some milk tea were higher than 2.5 mg/kg, the set limit in national standard.
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