本研究利用50对来自茶系的SSR引物对41份茶组资源进行PCR扩增,研究不同类型SSR引物的通用性和多样性指数。研究结果表明,茶系的SSR引物在茶组植物中的通用性高,其中EST-SSR通用性多态位点的比例达到60%。另外,利用SSR标记技术对茶组材料的遗传多样性进行分析。不同位点在种间的等位基因数为2~6个,平均每个位点4.21个等位基因。通过UPGMA方法构建了茶组12个种的遗传进化关系图。
For the purpose of investigating the transferability and polymorphism information content (PIC) of different types of SSR primers, PCR amplification for 41 accessions from section thea was conducted in this investigation. The results showed that the SSR primers form Ser. Sinensis were highly transferable among section Thea. The polymorphic and transferable primers account for 60% of EST-SSR markers. Then the genetic diversity among 12 species of section Thea was evaluated through SSR analysis. The number of alleles in different loci ranged from 2 to 6, with average 4.21 per locus. The phyolgenetic dendrogram was constructed by the UPGMA.
[1] 张宏达. 山茶属植物的系统研究[J]. 中山大学学报(自然科学版)论丛, 1981(1): 1~124.
[2] 张宏达. 茶树的系统分类[J]. 中山大学学报(自然科学版),1981(1): 87~99.
[3] 张宏达. 茶叶植物资源的订正[J]. 中山大学学报(自然科学版), 1984(1): 1~12.
[4] 闵天禄. 山茶属茶组植物的订正[J]. 云南植物研究, 1992, 14(2): 115~132.
[5] 王丽鸳, 成浩, 周健. 茶树DNA分子标记及基因工程研究进展[J]. 茶叶科学, 2004, 24(1): 12~17.
[6] 陈亮. 茶组植物的分子系统学研究[D]. 杭州: 浙江大学博士论文, 2002
[7] Powell W, Machray GC, Provan J.Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1996, 1: 215~222.
[8] Ma RC, Xie H, Xu Y, et al. Development of SSR markers for the phylogenetic analysis of almond trees from China and the Mediterranean region[J]. Genome, 2004, 47(6): 1091~1104.
[9] 庞晓明, 胡春根, 邓秀新. 用SSR标记研究柑橘属及其近缘属植物的亲缘关系[J]. 2003, 30(1): 81~87.
[10] 吴晓雷, 贺超英, 陈受宜, 等. 用SSR分子标记研究大豆属种间亲缘进化关系[J]. 遗传学报, 2001, 28(4): 359~366.
[11] Gao LF, Jing RL, Huo NX, et al. One hundred and one new microsatellite loci derived from ESTs(EST-SSRs) in bread wheat[J]. Theor Appl Genet, 2004, 108, 1392~1400.
[12] Arnold C, Rossetto M, Mcnally Jody, et al. The application of SSRs characterized for grape ( Vitis vinifera) to conservation studied in Vitaceae[J]. American Journal of Botany, 2002, 89(1) :22~281.
[13] Peakall R, Gilmore S, Keys W, et al. Cross species amplification of soybean ( Glyci ne max ) simple sequence repeats (SSRs) within the Genus and other legume genera: implications for the transferability of SSRs in plants[J]. Mol Biol Evol, 1998, 15(10): 1275~1287.
[14] 刘本英, 王平盛, 周红杰, 等. 云南茶组植物ISSR-PCR反应体系的建立[J]. 云南农业大学学报, 2006(增): 21~25.
[15] Freeman S, West J, James C, et al. Isolation and characterization of highly polymorphic microsatellites in tea (Camellia sinensis)[J].Molecular Ecology Notes, 2004, 4: 324~326.
[16] Kaundun SS,Matsumoto S.Heterologous nuclear and chloroplast, microsatellite amplification and variation in tea, Camellia sinensis[J], Genome, 2002, 45: 1041~1048.
[17] 金基强, 崔海瑞, 龚晓春, 等. 用EST-SSR 标记对茶树种质资源的研究[J]. 遗传, 2007, 29(1): 103~108.
[18] 刘振, 王新超, 赵丽萍, 等. 基于EST-SSR 的西南茶区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1): 100~110.
[19] Charters YM, Wilkinson MJ.The use of self-pollinated progenies as“in-groups”for the genetic characterization of cocoa germplasm[J]. Theor Appl Genet, 2000, 100: 160~166.
[20] Liu K, Muse S V.PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics Applications Note, 2005, 21(9): 2128~2129.
[21] Nei M, Li WH.Mathematical modeling for studying genetic variation in terms of restriction endonuclease[J]. Proc Natl Acad Sci (USA), 1979, 74: 5269~5273.
[22] Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980, 32: 314~331.
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