研究应用酶联免疫方法,快速检测茶叶中硫丹及硫丹硫酸酯。茶叶提取后,不净化,用PBS缓冲液稀释500倍后,进行酶联免疫方法测定,方法平均回收率为86.6%~91.1%;经SPE净化后,回收率68.7%~123%。标准曲线IC50为2βµg/L,线性范围1~10βµg/L。直接提取法最低检测限2βmg/kg,净化法为0.06βmg/kg。经气相色谱/质谱联用方法验证,灵敏度、精密度和准确度均符合快速检测要求。该法具有快速、简便、低耗、易操作等特点。
吕潇
,
任凤山
,
王文博
,
杜红霞
,
李慧冬
,
丁蕊艳
,
朱端卫
,
LEENanjuA
,
SHEILDRoss
,
KENNEDYIvanR
. 茶叶中硫丹及硫丹硫酸酯的ELISA快速测定[J]. 茶叶科学, 2006
, 26(2)
: 141
-146
.
DOI: 10.13305/j.cnki.jts.2006.02.013
A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1βµg/L to 10βµg/L. The detection limit of crude extract was 2βmg/kg and the purified was 0.06βmg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea.
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