研究了茶毛虫核型多角体病毒（Euproctis pseudoconspersa nuclear polyhedrosis virus,简称EpNPV）的室内外增殖、分离粗提,以及对茶毛虫（Euproctis pseudoconspersa Strand）室内毒力测定、田间防治效果和病毒电镜检测。采用室内离体茶树嫩枝水培,106βPIB/ml EpNPV喷雾接种2~3龄茶毛虫,或大田直接喷雾处理2~3龄茶毛虫,在罹病幼虫液化前及时收集虫尸,完成病毒增殖。106βPIB/ml、107βPIB/ml、108βPIB/ml EpNPV室内处理第12天致病率分别为43.60％、76.13％和64.68％;第14天致病率分别为59.17％、95.71％和95.70％。大田试验107βPIB/ml喷雾处理第15天防治效果达78.11%。经透射电镜观察田间防治大量感病茶毛虫虫尸,证实为EpNPV致病死亡。

The reproduction, extraction, pathogenicity to Tea Tussork Moth and electron micrograph testing of Euproctis pseudoconspersa Nuclear Polyhedrosis Virus(EpNPV) was investigated. The EpNPV was propagated through Inoculating 2~3 instar larvae of Euproctis pseudoconspersa, by raged with EpNPV at the concentration 10⁶βPIB/ml, or inoculating 2~3 instar larvae in field condition. Inoculation the larvae with 10⁶βPIB/ml, 10⁷βPIB/ml, and 10⁸βPIB/ml of EpNPV, the corrected mortalities were 43.60％, 76.13％ and 64.68％ on day 12 respectively, and reached 59.17％, 95.71％, and 95.70％ on day 14 respectively. The mortality was 78.11% on day 15 treated with EpNPV(10⁷βPIB/ml) under field condition. Virus of the EpNPV from the field dead larvae could be watched through the electron microscope.