欢迎访问《茶叶科学》,今天是

茶叶科学 ›› 2006, Vol. 26 ›› Issue (4): 265-269.doi: 10.13305/j.cnki.jts.2006.04.006

• • 上一篇    下一篇

茶毛虫核型多角体病毒对茶毛虫的致病性研究

厉晓腊, 金轶伟, 柴一秋*, 陈官菊, 刘又高   

  1. 浙江省亚热带作物研究所, 浙江 温州 325005
  • 收稿日期:2006-08-17 修回日期:2006-09-07 出版日期:2006-12-25 发布日期:2019-09-11
  • 通讯作者: * 柴一秋, E-mail:chai-yiqiu@yeah.net
  • 作者简介:厉晓腊(1977- ),女,浙江永嘉人,主要从事植保等工作
  • 基金资助:
    温州市科技局资助项目(N2005A015)

The Pathogenicity of Euproctis Pseudoconspersa Nuclear Polyhedrosis Virus on the Larvae of Tea Tussork (Euproctis pseudoconspersa Strand)

LI Xiao-la, JIN Yi-wei, CHAI Yi-qiu*, CHEN Guan-ju, LIU You-gao   

  1. Zhejiang Institute of Subtropical Crops , Wenzhou 325005, China
  • Received:2006-08-17 Revised:2006-09-07 Online:2006-12-25 Published:2019-09-11

摘要: 研究了茶毛虫核型多角体病毒(Euproctis pseudoconspersa nuclear polyhedrosis virus,简称EpNPV)的室内外增殖、分离粗提,以及对茶毛虫(Euproctis pseudoconspersa Strand)室内毒力测定、田间防治效果和病毒电镜检测。采用室内离体茶树嫩枝水培,106βPIB/ml EpNPV喷雾接种2~3龄茶毛虫,或大田直接喷雾处理2~3龄茶毛虫,在罹病幼虫液化前及时收集虫尸,完成病毒增殖。106βPIB/ml、107βPIB/ml、108βPIB/ml EpNPV室内处理第12天致病率分别为43.60%、76.13%和64.68%;第14天致病率分别为59.17%、95.71%和95.70%。大田试验107βPIB/ml喷雾处理第15天防治效果达78.11%。经透射电镜观察田间防治大量感病茶毛虫虫尸,证实为EpNPV致病死亡。

关键词: 茶毛虫, 核型多角体病毒(EpNPV), 致病性, 增殖, 生物防治

Abstract: The reproduction, extraction, pathogenicity to Tea Tussork Moth and electron micrograph testing of Euproctis pseudoconspersa Nuclear Polyhedrosis Virus(EpNPV) was investigated. The EpNPV was propagated through Inoculating 2~3 instar larvae of Euproctis pseudoconspersa, by raged with EpNPV at the concentration 106βPIB/ml, or inoculating 2~3 instar larvae in field condition. Inoculation the larvae with 106βPIB/ml, 107βPIB/ml, and 108βPIB/ml of EpNPV, the corrected mortalities were 43.60%, 76.13% and 64.68% on day 12 respectively, and reached 59.17%, 95.71%, and 95.70% on day 14 respectively. The mortality was 78.11% on day 15 treated with EpNPV(107βPIB/ml) under field condition. Virus of the EpNPV from the field dead larvae could be watched through the electron microscope.

Key words: Tea Tussork Moth(Euproctis pseudoconspersa Strand), Euproctis Pseudoconspersa Nuclear Polyhedrosis Virus (EpNPV), Pathogenicity, reproduction, biocontrol

中图分类号: