为研究茶多酚（TP）与油菜花粉提取物对前列腺癌细胞生长抑制效果的差异,应用细胞培养技术、MTT显色试验测定（IC50）法、荧光显微镜观察癌细胞形态变化。结果表明,除花粉醇溶性提取物（B）对前列腺癌细胞没有明显抑制作用外,花粉水溶性提取物（A）及A（B）与TP的等比例混合物均有显著的抑制作用,A、A+TP、B+TP和TP的IC50依次为1470、752、82、45 μg/mL,B+TP对PC-3的生长均有抑制作用,且成剂量效应和时间效应关系,经B+TP处理的PC-3癌细胞,用荧光显微镜可以看到癌细胞的凋亡小体。
To study the effect of tea polyphenols (TP) or rape pollen extract (RPE) on the inhibition of PC-3 cell in vitro. MTT assay was used to evaluate the inhibition activity (IC50) on PC-3 proliferation, and fluorescence microscope was used to observe the apotosis cells. Results showed that water-soluble extract of pollen (A), ethanol-soluble pollen extract (B) and TP were proved to be effectively inhibited on the prostate cancer cell PC-3. The IC50 of A, A+TP, B+TP and TP were 1470, 752, 82 μg/mL and 45 μg/mL, respectively. B+TP showed effect-dose and effect-time relationship. B+TP was also found to induce cell apotosis as shown by changes in nuclear morphology and DNA fragmentation.
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