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茶叶科学 ›› 2010, Vol. 30 ›› Issue (5): 379-383.doi: 10.13305/j.cnki.jts.2010.05.009

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茶多酚与油菜花粉提取物对前列腺癌细胞生长抑制效果比较研究

张星海1, 龚恕1, 王岳飞2, 周晓红1   

  1. 1. 浙江经贸职业技术学院应用工程系,浙江 杭州 310018;
    2. 浙江大学茶学系,浙江 杭州 310029
  • 收稿日期:2009-12-01 修回日期:2010-04-26 发布日期:2019-09-11
  • 作者简介:张星海(1973— ),男,安徽合肥人,讲师,硕士,主要从事天然产物的提取分离和生物学活性研究。zjuxy2001@163.com。

Comparation on the Inhibitory Effect of Tea Polyphenols and Rape Pollen Extract on Prostate Cancer Cell

ZHANG Xing-hai1, GONG Shu1, WANG Yue-fei2, ZHOU Xiao-hong1   

  1. 1. The Department of Applied Engineering, Zhejiang Economic and Trade Polytechnic, Hangzhou 310018, China;
    2. Department of Tea Science, Zhejiang University, Hangzhou 310029, China
  • Received:2009-12-01 Revised:2010-04-26 Published:2019-09-11

摘要: 为研究茶多酚(TP)与油菜花粉提取物对前列腺癌细胞生长抑制效果的差异,应用细胞培养技术、MTT显色试验测定(IC50)法、荧光显微镜观察癌细胞形态变化。结果表明,除花粉醇溶性提取物(B)对前列腺癌细胞没有明显抑制作用外,花粉水溶性提取物(A)及A(B)与TP的等比例混合物均有显著的抑制作用,A、A+TP、B+TP和TP的IC50依次为1470、752、82、45 μg/mL,B+TP对PC-3的生长均有抑制作用,且成剂量效应和时间效应关系,经B+TP处理的PC-3癌细胞,用荧光显微镜可以看到癌细胞的凋亡小体。

关键词: 茶多酚, 油菜花粉提取物, 前列腺癌, 凋亡

Abstract: To study the effect of tea polyphenols (TP) or rape pollen extract (RPE) on the inhibition of PC-3 cell in vitro. MTT assay was used to evaluate the inhibition activity (IC50) on PC-3 proliferation, and fluorescence microscope was used to observe the apotosis cells. Results showed that water-soluble extract of pollen (A), ethanol-soluble pollen extract (B) and TP were proved to be effectively inhibited on the prostate cancer cell PC-3. The IC50 of A, A+TP, B+TP and TP were 1470, 752, 82 μg/mL and 45 μg/mL, respectively. B+TP showed effect-dose and effect-time relationship. B+TP was also found to induce cell apotosis as shown by changes in nuclear morphology and DNA fragmentation.

Key words: tea polyphenols (TP), rape pollen extract (RPE), prostate cancer, apotosis

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