研究从毛栓菌中分离得到的多酚氧化酶（PPO）用于茶黄素的体外氧化制备。结果表明毛栓菌胞外PPO较适的反应温度范围在28~36℃之间;最适pH值为5.2;恒温反应30βmin内均表现出较高的活性和稳定性;50%的硫酸铵可以沉淀粗酶液中96.0%的PPO。将毛栓菌PPO加入到儿茶素反应液中进行双液相反应,可得到10.19%的茶黄素,与茶鲜叶来源的PPO相比,毛栓菌PPO制备茶黄素的总含量偏低,但其中TF-3-G的比例较高,达到总茶黄素的68.10%,占脂型茶黄素总量的92.08%。
：The investigation on the oxidative preparation in vitro of theaflavin by using the polyphenol oxidase(PPO) from Trametes trogii was conducted. Results showed that the optimum reaction temperature for PPO produced by Trametes trogii was ranged from 28℃to 36℃, and the optimum pH value for PPO was 5.2. The PPO showed higher activity and stability during the first 30 minutes when the reaction temperature was constant. 96.2% PPO could be precipitated from the crude enzyme solution when 50% Ammonium sulfate was added to. Added PPO (from Trametes trogii) to two-liquid-phase of catechin reaction system, the TFs was produced 10.19%. Compared with the contrast (PPO from fresh tea leaves), the total quantity of TFs (PPO from Trametes trogii) was lower,but the ratio of TF-3-G was much higher when PPO come from Trametes trogii, and the content of TF-3-G reached 68.1%. It occupied 92.08% of the total ester TFs.
[1] Shengmin Sang, Joshua D Lambert, Shiying Tian, et al. Enzymatic synthesis of tea theaflavin derivatives and theiranti-inflammatory and cytotoxic activities[J]. Bioorganic & Medicinal Chemistry, 2004, 12: 459~467.
[2] 陈虎, 胡英, 周睿, 等. 茶黄素的抗氧化机理的研究进展[J]. 茶叶科学, 2005, 25(4): 237~241.
[3] 蔡飞, 李彩蓉, 吴基良, 等. 茶黄素对大鼠脑缺血再灌注炎性细胞因子的影响[J]. 中药药理与临床, 2007, 23(1): 23~24.
[4] 李府, 尚希福, 谢涛. 茶黄素对兔骨髓基质干细胞向脂肪细胞诱导分化的影响[J]. 中国组织工程研究与临床康复, 2008, 12(16): 3061~3064.
[5] 吴红梅. 多酚氧化酶酶源筛选及酶法制取茶色素研究[D]. 合肥: 安徽农业大学硕士学位论文, 2004.
[6] 刘仲华, 黄建安, 施兆鹏. 黑茶初制中主要酶类的变化[J]. 茶叶科学, 1991, 11(增): 17~22.
[7] 谷记平, 刘仲华, 黄建安, 等. 单双液相下不同多酚氧化酶酶源对酶性合成茶黄素的研究[J]. 茶叶科学, 2007, 27(1): 76~82.
[8] 胡耀武. 双液相发酵应用于茶色素形成与制取的研究[D]. 合肥: 安徽农业大学硕士论文, 1998.
[9] 周长路, 王宜磊. 多孔菌Polyporus W38多酚氧化酶的性质研究[J]. 生物技术, 2001, 11(3): 14~17.
[10] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶叶科学, 2004, 24(1): 53~59.
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