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茶叶科学 ›› 2008, Vol. 28 ›› Issue (5): 326-330.doi: 10.13305/j.cnki.jts.2008.05.001

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毛栓菌多酚氧化酶酶学性质及茶黄素的酶促合成研究

李适, 刘仲华, 黄建安, 王坤波, 施玲*   

  1. 湖南农业大学茶学教育部重点实验室,湖南 长沙410128
  • 收稿日期:2008-05-05 出版日期:2008-10-15 发布日期:2019-09-12
  • 通讯作者: *,miaolingshi@*263.net
  • 作者简介:李适(1981— ),女,湖南醴陵人,主要从事植物功能成分研究与开发。
  • 基金资助:
    科技部十一五国际合作重点项目(2006DFA33220)

Research on Enzymatic Nature of Polyphenol Oxidase from Trametes Trogii and the Enzymatic Synthesis of Theaflavins

LI Shi, LIU Zhong-hua, HUANG Jian-an, WANG Kun-bo, SHI Ling*   

  1. Key lab of tea science of china ministry of education, changsha 410128, China
  • Received:2008-05-05 Online:2008-10-15 Published:2019-09-12

摘要: 研究从毛栓菌中分离得到的多酚氧化酶(PPO)用于茶黄素的体外氧化制备。结果表明毛栓菌胞外PPO较适的反应温度范围在28~36℃之间;最适pH值为5.2;恒温反应30βmin内均表现出较高的活性和稳定性;50%的硫酸铵可以沉淀粗酶液中96.0%的PPO。将毛栓菌PPO加入到儿茶素反应液中进行双液相反应,可得到10.19%的茶黄素,与茶鲜叶来源的PPO相比,毛栓菌PPO制备茶黄素的总含量偏低,但其中TF-3-G的比例较高,达到总茶黄素的68.10%,占脂型茶黄素总量的92.08%。

关键词: 茶黄素, 多酚氧化酶, 毛栓菌, 酶促合成

Abstract: :The investigation on the oxidative preparation in vitro of theaflavin by using the polyphenol oxidase(PPO) from Trametes trogii was conducted. Results showed that the optimum reaction temperature for PPO produced by Trametes trogii was ranged from 28℃to 36℃, and the optimum pH value for PPO was 5.2. The PPO showed higher activity and stability during the first 30 minutes when the reaction temperature was constant. 96.2% PPO could be precipitated from the crude enzyme solution when 50% Ammonium sulfate was added to. Added PPO (from Trametes trogii) to two-liquid-phase of catechin reaction system, the TFs was produced 10.19%. Compared with the contrast (PPO from fresh tea leaves), the total quantity of TFs (PPO from Trametes trogii) was lower,but the ratio of TF-3-G was much higher when PPO come from Trametes trogii, and the content of TF-3-G reached 68.1%. It occupied 92.08% of the total ester TFs.

Key words: theaflavins (TFs), polyphenol oxidase(PPO), trametes trogii, enzymatic synthesis

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