通过水培实验,研究了茶树(3个品种)受铬(Cr3+)、镉(Cd2+)毒害的表观症状以及铬、镉在茶树体内的分布、积累特性。结果表明:铬(20~100 mg/L)、镉(1~5 mg/L)胁迫下,茶树出现的毒害症状和对新梢生长的抑制效应因胁迫浓度、时间和品种而异。茶树各器官的铬、镉含量总体随铬、镉胁迫浓度的增加而增加,并且与胁迫浓度呈极显著或显著正相关。铬在茶树体内由高到低的分布次序是:吸收根>主根>主茎>枝条>老叶>新梢(一芽二叶);镉则为:吸收根>主根>主茎>新梢(一芽二叶)>枝条>老叶。根部吸收的铬、镉元素大部分被吸收根所固定,向地上部运输的比例较低,但镉在茶树体内的活性和迁移能力强于铬,运输到地上部的镉极易迁移到新梢中。新梢不同叶位镉含量的高低顺序为:第一叶>第二叶>第三叶>第四叶,新梢中的镉有明显向上富集的趋势。不同品种的茶树对铬、镉的吸收、积累和迁移能力有一定差异。
The accumulation and distribution of Cr3+ and Cd2+ in hydroponic cultured different tea cultivars including Mingshan131, Mingshan213 and Fudingdabaicha and their toxic symptoms to these plants had been studied. The results indicated that the inhibitory effects and toxic symptoms of Cr3+ and Cd2+ on tea plants new shoots and new shoots of tea plants changed with different stress concentration, time and tea cultivars under Cr3+(20~100 mg/L), Cd2+(1~5 mg/L)stress. The concentrations of Cr and Cd in tea plants increased with the increasing of Cr3+ and Cd2+ stress or the prolonging of treated times. The total content of chromium and cadmium in all organs of tea plant was significantly positively correlated with the chromium, cadmium concentrations and the distribution order of Cr in the tea plants was: fine roots> main root> main stem> branches> old leaves>new shoots (one bud two leaves) while that of Cd was: fine roots> main root>main stem>new shoots (one bud two leaves)> branches> old leaves. Chromium and cadmium elements absorbed by roots were fixed in the fine root and the proportion of transport to upper was low, but the activity and transfer ability of cadmium in tea are stronger than that of chromium. Cadmium transported to the ground and could easily be migrated to new shoots. The order of Cadmium content in different leaf location of new shoots was: the bud> the second >the third >the fourth leaves. Cadmium had an obvious upward trend. The absorption, accumulation and migration ability of chromium and cadmium in tea plants changed with the different tea cultivars.
[1] 顾公望, 张宏伟. 微量元素与恶性肿瘤[M]. 上海: 上海科学技术出版社, 1993: 199~205.
[2] 陈怀满. 土壤-植物系统中的重金属污染[M]. 北京:科学出版社, 1996: 71~167.
[3] 许嘉琳, 杨居荣. 陆地生态系统中的重金属[M]. 北京: 中国环境科学出版社, 1995: 45-55, 333-342.
[4] 石元值, 阮建云, 马立锋, 等. 茶树中镉、砷元素的吸收累积特性[J]. 生态与农村环境学报, 2006, 2(3): 70~75.
[5] 石贵玉. 重金属Cr6+水稻幼苗的毒害效应[J]. 广西科学, 2004, 11(2): 154~156.
[6] 张义贤. 重金属对大麦(Hordeum nulgare)毒性的研究[J]. 环境科学学报, 1997, 17(2): 199~205.
[7] 何江华, 何文彪, 杜应琼, 等. Cr(Ⅵ)对蔬菜生长及其在蔬菜体内积累的影响[J]. 农业环境科学学报, 2005, 24(增刊): 1~4.
[8] 洪仁远, 蒲长辉. 镉对小麦幼苗的生长和生理生化反应的影响[J]. 华北农学报, 1991, 6(3): 70~75.
[9] 徐照丽, 吴启堂, 依艳丽. 不同品种菜心对镉抗性的研究[J]. 生态学报, 2002, 22(4): 571~576.
[10] 夏建国, 兰海霞. 镉胁迫对蒙山茶树生长及叶片生理指标的影响[J]. 茶叶科学, 2008, 28(1): 56~61.
[11] 童启庆. 茶树栽培学[M]. 中国农业出版社, 2000: 344.
[12] 唐咏, 王萍萍, 张宁. 植物重金属毒害作用机理研究现状[J]. 沈阳农业大学学报, 2006, 37(4): 551~555.
[13] 熊愈辉, 杨肖娥. 镉对植物毒害与植物耐镉机理研究进展[J]. 安徽农业科学, 2006, 34(13): 2969~2971.
[14] 仇硕, 张敏, 孙延东, 等. 植物重金属镉(Cd2+)吸收、运输、积累及耐性机理研究进展[J]. 西北植物学报, 2006, 26(12): 2615~2622.
[15] 张义贤. 三价铬和六价铬对大麦毒害效应的比较[J]. 中国环境科学, 1997, 17(6): 565~568.
[16] 张金彪, 黄维南, 柯玉琴. 草莓对镉的吸收积累特性及调控研究[J]. 园艺学报, 2003, 30(5): 514~518.
[17] 杨德, 程永安, 吕金印. 铬在南瓜体内的分布与积累[J]. 植物生理学通讯, 2006, 42(5): 958~960.
[18] 孙游云. 铬对植物体生长生理的影响及其在植物体中的积累规律[J]. 环境污染与防治, 2001, 1(20): 45~46.
[19] 宋阿琳, 娄运生, 梁永超. 不同水稻品种对铜镉的吸收与耐性研究[J]. 中国农学通报, 2006, 22(9): 408~411.
[20] 赵秀兰, 李彦峨. 烟草积累与忍耐镉的品种差异[J]. 西南大学学报(自然科学版), 2007, 29(3): 110~114.
[21] 周希琴, 吉前华. 铬胁迫下不同品种玉米种子和幼苗的反应及其与铬积累的关系[J]. 生态学杂志, 2005, 24(9): 1048~1052.