本文对茶氨酸在制备色谱分离时其在反相C18色谱分离柱上的热力学及动力学色谱行为进行了研究。采用前沿分析法对其吸附等温线进行表征,对其在不同超载方式和超载程度时的超载点进行定量表达;并研究在超载状态下茶氨酸的各分离参数的变化关系及其对分离操作条件的影响。结果表明,为提高茶氨酸产率,以采用体积超载方式为好,并可采用峰宽对体积超载进行表征,当W增宽15%~20%时,表示色谱柱处于体积超载状态;为提高制备量,可采用较大颗粒的填料。
Retention characteristics of theanine were investigated on C18 column for preparative high performance liquid chromatography (prep-HPLC). Based on frontal analysis and adsorption isotherm, effects of chromatographic conditions to separation were studied on overload injection. Results showed that the separation effect of volume overload is better than that of concentration overload. And large granule solid phase is recommended for large-scale purification of theanine on C18 column.
[1] Sagesaka Y, Kakuda T.Phaarm acdogical effect of theanine[J]. The Organizing committee of ISTS, 1992, 362~365.
[2] Yokozawa T, Dong E, Chung H Y. Inhibitory effect of green tea on injury to a cultured renal epithelial cell line, LLC-PK1[J]. Biosci Biotechnol Biochem1997 Jan, 61(1): 204-6.
[3] Kakuda T.,Neuroprotective effects of the green tea components theanine and catechins[J]. Biol Pharm Bull2002 Dec, 25(12): 1513-8.
[4] Sadzuka Y, Yamashita Y, Kishimoto S. Glutamate transporter mediated increase of antitumor activity by theanine, an amino acid in green tea[J]. Yakugaku Zasshi, 2002 Nov, 122(11): 995-9.
[5] JP07,173,059,JP08,73,350(1990).
[6] http://www.nutrasolution.com/clinicalresearch.
[7] Tsuda,K.JAFAN, 1995, 14: 259-265.
[8] 佐冢泰之. 茶叶成分茶氨酸对对阿霉素抗癌作用及副作用的影响(M);《国外医学中医中药分册》, 1996, 18(5): 3~5.
[9] Kakuda T, Yanase H, Utsunomiya K. Protective effect of gamma-glutamylethylamide (theanine) on ischemic delayed neuronal death in gerbils[J]. Neurosci Lett2000 Aug 11, 289(3): 189-92.
[10] Ying Zhang, Bo Chen, Zhiqiang Huang.Preparative Isolation and Purification of L-Theanine by HPLC[J]. J Liq. Chromatogr. & Related Technol,2004, 27(5): 875~884.
[11] G Guiochon.Fundamentals of Preparative and Nonlinear Chromatogaphy[M]. Academy Press, Boston, 1994: 595~657.
[12] Jandera P, Komers D, Guiochon G. Optimization of the recovery yield and of the production rate in overloaded gradient-elution reversed-phase chromatography[J]. J Chromatogr A, 1998 Feb 13, 796(1):115-27
[13] Gritti F, Felinger A, Guiochon G. Overloaded gradient elution chromatography on heterogeneous adsorbents in reversed-phase liquid chromatography[J]. J Chromatogr A, 2003 Oct 31, 1017(1-2): 45-61.
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