通过分离培养基和分离方法的筛选,得到了从叶面分离Bt的有效方法。用此法从25种植物叶面分离出54株Bt,利用茶尺蠖、茶小绿叶蝉和茶橙瘿螨作为靶标昆虫,筛选对茶树主要害虫有毒力菌株,得到了对鳞翅目有效的菌株10株,对同翅目有效的菌株4株,但所有测试菌株均对茶橙瘿螨无效。
张灵玲
,
林晶
,
骆兰
,
方昉
,
黄天培
,
徐金汉
,
吴光远
,
王庆森
,
关雄
. 叶面分离Bt及对茶树主要害虫高毒力菌株的筛选[J]. 茶叶科学, 2005
, 25(1)
: 56
-60
.
DOI: 10.13305/j.cnki.jts.2005.01.010
An isolation method was developed with comparing different selective culture media and 54 Bt strains were isolated from 25 species of plants in China. The bioassay was conducted by use of Ectropis obliqua, Empoasca vitis and Acaphylla theae as target insects. It showed that there were 10 strains effective to Lepidoptera and 4 to Homoptera but they were all ineffective to Acaphylla theae mites.
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