采用不同的激素种类、浓度和浸蘸时间诱导茶树组培苗在温室内生根,观察不同激素强度作用下生根率的动态变化,通过优化技术参数,建立组培苗的温室直接生根技术体系。结果表明,在100βmg/L的IBA短时浸蘸处理70天后,小苗的成活率为66.7%,生根率达到60%,约为成活小苗的90%左右,平均发根数为9.4条,根长为3.64βcm。该技术的建立有助于实现茶树组织培养快速繁殖工厂化育苗技术的商业化推广。
Induction on direct rooting of tea tissue culture microshoots with different levels of phytohormone was expermented in greenhouse. We screened out the optimum levels of hormone, analyzed the dynamics of the percentage of rooted seedlings under different hormone levels, and established the technology of direct rooting of tea microshoots in greenhouse. The results showed that the surviving rate of seedlings was 66.7%, the rooting rate was 60%, about 90% of those survived seedlings, the average amounts of root was 9.4, and the length was 3.64βcm at the 70th day after instantaneous dipping treatment of 100βmg/L IBA. This technology might be developed for commercial application of tea tissue culture procedure and the industrialized incubation of tea seedlings.
[1] 徐振华, 王学勇, 李敬川, 等. 试管苗瓶外生根研究进展[J]. 中国农学通报, 2002, 18(4): 84~87.
[2] 杨素娟, 王玉书, 王立. 茶树未成熟胚培养根的诱导及形态发生[J]. 中国茶叶, 1989, 2: 14~15.
[3] 冯学赞, 陈文龙, 王玉珍, 等. 组培苗气培生根法的研究[J]. 林业科技通讯, 1998, 25(4): 405~407.
[4] 孙仲序, 刘静, 王玉军, 等. 果树组培苗瓶外滤纸桥生根技术研究[J]. 园艺学报, 2001, 28(4): 345-347.
[5] Lilia Alcaraz-Melendez, Sergio Real-Cosio, Manuel L, et al. Morphological comparison of damiana (Turnera diffusa, Willd.) regenerated in vitro from leaves cultured in solid medium and liquid cultures[J]. Scientia Horticulturae, 2002, 96: 293~301.
[6] Tapan Kumar Mondal, Amita Bhattacharya, Anil Sood, et al. Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron[J]. Plant Growth Regulation, 1998, 26: 57~61.
[7] Tapan K. Mondal, Amita Bhattacharya, Malathi Laxmikumaran, et al. Recent advances of tea (Camellia sinensis) biotechnology[J]. Plant Cell, Tissue and Organ Culture, 2004, 76: 195~254.
浙公网安备 33019902000101号 
