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茶叶科学 ›› 2005, Vol. 25 ›› Issue (4): 265-269.doi: 10.13305/j.cnki.jts.2005.04.005

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激素处理对茶树组培苗温室内直接诱导生根的影响

周健, 成浩*, 王丽鸳   

  1. 农业部茶叶化学工程重点开放实验室,中国农业科学院茶叶研究所,浙江 杭州 310008
  • 出版日期:2005-12-25 发布日期:2019-09-16
  • 通讯作者: chenghao@mail.tricaas.com
  • 作者简介:周健(1979— )男,研究实习员,主要从事茶叶生物技术研究工作。
  • 基金资助:
    浙江省科技计划项目“茶树新品种快速繁育体系创新研究”资助项目,资助号:02402090

Effect of Phytohormone Induction on Direct Rooting of Tea Plant (Camellia sinensis) Microshoots in Greenhouse

ZHOU Jian, CHENG Hao*, WANG Li-yuan   

  1. Key Lab of Tea Chemical Engineering, Ministry of Agriculture;Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Online:2005-12-25 Published:2019-09-16

摘要: 采用不同的激素种类、浓度和浸蘸时间诱导茶树组培苗在温室内生根,观察不同激素强度作用下生根率的动态变化,通过优化技术参数,建立组培苗的温室直接生根技术体系。结果表明,在100βmg/L的IBA短时浸蘸处理70天后,小苗的成活率为66.7%,生根率达到60%,约为成活小苗的90%左右,平均发根数为9.4条,根长为3.64βcm。该技术的建立有助于实现茶树组织培养快速繁殖工厂化育苗技术的商业化推广。

关键词: 茶树, 组织培养, 生根, 生长素

Abstract: Induction on direct rooting of tea tissue culture microshoots with different levels of phytohormone was expermented in greenhouse. We screened out the optimum levels of hormone, analyzed the dynamics of the percentage of rooted seedlings under different hormone levels, and established the technology of direct rooting of tea microshoots in greenhouse. The results showed that the surviving rate of seedlings was 66.7%, the rooting rate was 60%, about 90% of those survived seedlings, the average amounts of root was 9.4, and the length was 3.64βcm at the 70th day after instantaneous dipping treatment of 100βmg/L IBA. This technology might be developed for commercial application of tea tissue culture procedure and the industrialized incubation of tea seedlings.

Key words: Tea plant (Camellia sinensis), Tissue culture, Direct rooting, Phytohormone

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