茶树果胶甲酯酶及其抑制子家族基因的鉴定及CsPME55参与氟胁迫响应的功能分析

doi:10.13305/j.cnki.jts.2024.06.008

摘要/Abstract

摘要： 茶树（Camellia sinensis）具有氟（Fluoride,F）富集特性,且F主要富集在细胞壁组分的果胶中;果胶甲酯酶（Pectin methylesterase,PME）及其抑制子PMEI（Pectin methylesterase inhibitor）能够催化果胶的修饰进而影响细胞壁特性,参与植物生长发育、逆境响应等过程。从茶树舒茶早基因组中鉴定了85个CsPMEs和56个CsPMEIs成员,分别包括4个和5个亚族;不同亚族可能因基因结构、保守基序和表达模式等不同呈现功能分化。荧光定量结果表明,CsPME3a、CsPME55、CsPMEI1和CsPMEI3在福鼎大白茶成熟叶中被F处理显著诱导表达。此外,过表达CsPME55缓解了F胁迫对拟南芥根系生长的抑制作用,推测CsPME55可能参与茶树F胁迫调控,研究结果将为进一步研究PME和PMEI家族基因参与F响应的功能奠定基础。

关键词: 茶树, 氟, 果胶甲酯酶, 果胶甲酯酶抑制子, 基因表达, 功能分析

Abstract: Tea plant (Camellia sinensis) has fluoride (F) enrichment characteristics, and F is mainly enriched in the cell wall component pectin. Pectin methylsterase (PME) and its inhibitor PMEI can catalyze the modification of pectin, thereby affecting cell wall characteristics and participating in the regulation of processes like plant growth and development, stress response and so on. In this study, 85 CsPMEs and 56 CsPMEIs were identified from the C. sinensis ‘Shuchazao’ genome, which were divided into 4 and 5 subgroups, respectively. Distinct subgroups may exhibit functional distinction due to varied gene architectures, conserved motifs and expression patterns. Quantitative real-time PCR (qRT-PCR) analysis reveals that the expression levels of CsPME3a, CsPME55, CsPMEI1 and CsPMEI3 were significantly induced in the mature leaves of ‘Fuding Dabaicha’ under F treatment. Moreover, overexpression of CsPME55 alleviated Arabidopsis root growth inhibition induced by F stress, suggesting its potential role in F stress regulation in tea plants. These findings could pave the way for further research on the functional involvement of PME and PMEI gene families in F response.

Key words: Camellia sinensis, fluoride, pectin methylesterase, pectin methylesterase inhibitor, gene expression, functional analysis

中图分类号:

徐文鸾, 温晓菊, 贾雨轩, 倪德江, 王明乐, 陈玉琼. 茶树果胶甲酯酶及其抑制子家族基因的鉴定及CsPME55参与氟胁迫响应的功能分析[J]. 茶叶科学, 2024, 44(6): 869-886. doi: 10.13305/j.cnki.jts.2024.06.008.

XU Wenluan, WEN Xiaoju, JIA Yuxuan, NI Dejiang, WANG Mingle, CHEN Yuqiong. Identification of Pectin Methylesterase and Its Inhibitory Subfamily Genes, and Functional Analysis of CsPME55 in Response to Fluoride Stress in Camellia sinensis[J]. Journal of Tea Science, 2024, 44(6): 869-886. doi: 10.13305/j.cnki.jts.2024.06.008.

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