茶叶科学 >

2025 , Vol. 45 >Issue 6: 1021 - 1035

DOI: https://doi.org/10.13305/j.cnki.jts.2025.06.008

研究报告

柚和茶树上柑橘始叶螨线粒体基因组特征及叶螨科系统发育分析

  • 陈世春 ,
  • 江宏燕 ,
  • 廖姝然 ,
  • 陈亭旭 ,
  • 王晓庆
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  • 重庆市农业科学院茶叶研究所,重庆 402160
陈世春,女,副研究员,主要从事茶树害虫分子生物学及防控技术方面的研究。

收稿日期: 2025-04-15

  网络出版日期: 2025-12-10

基金资助

国家现代农业产业技术体系（CARS-19）

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Mitochondrial Genome Characteristics and Phylogenetic Analysis of Eotetranychus kankitus on Pomelo and Tea Plants

  • CHEN Shichun ,
  • JIANG Hongyan ,
  • LIAO Shuran ,
  • CHEN Tingxu ,
  • WANG Xiaoqing
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  • Tea Research Institute of Chongqing Academy of Agricultural Sciences, Chongqing 402160, China

Received date: 2025-04-15

  Online published: 2025-12-10

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摘要

柑橘始叶螨（Eotetranychus kankitus Ehara）是一种危害多种作物的农业害螨,开展线粒体基因组的测定分析可明确其线粒体基因组结构特点,以及叶螨科物种的系统发育关系。通过PCR扩增、测序、组装和注释后获得柚和茶树上柑橘始叶螨的线粒体基因组序列,利用最大似然法和贝叶斯法构建了基于13个蛋白质编码基因（Protein coding gene,PCG）和2个核糖体RNA（Ribosomal RNA,rRNA）基因序列的叶螨科系统发育树。结果显示,柚和茶树上的柑橘始叶螨线粒体基因组大小均为13 032 bp,GenBank登录号分别为OQ955767和OQ955768,均包括13个PCG、2个rRNA基因、22个转运RNA（Transfer RNA,tRNA）基因和1个40 bp的控制区,基因排列完全一致,序列相似率较高,全基因组和各区域的碱基组成数值均较为接近。系统发育和遗传距离分析显示,两种寄主上的柑橘始叶螨在系统发育树上聚为一枝,两者之间遗传距离为0.068 0,远小于各自与叶螨科内其他物种之间的遗传距离。柑橘始叶螨在遗传距离和系统发育树上均与全爪螨属（Panonychus）物种存在较近的亲缘关系。研究通过线粒体基因组分析明确了柚和茶树上的柑橘始叶螨分化程度较低,寄主间转移风险较高,建议已有柑橘始叶螨发生以及周边有柑橘作物的茶园应加强田间监测工作。

关键词： 柑橘始叶螨; 系统发育; 线粒体基因组; 叶螨科

本文引用格式

陈世春 , 江宏燕 , 廖姝然 , 陈亭旭 , 王晓庆 . 柚和茶树上柑橘始叶螨线粒体基因组特征及叶螨科系统发育分析[J]. 茶叶科学, 2025 , 45(6) : 1021 -1035 . DOI: 10.13305/j.cnki.jts.2025.06.008

Abstract

Eotetranychus kankitus is an agricultural pest mite that damages many crops. Conducting mitochondrial genome analysis can clarify its structural characteristics and the phylogenetic relationships among species in the family of Tetranychidae species. The mitochondrial genome sequences of E. kankitus on pomelo and tea plants were obtained by PCR amplification, sequencing, assembly and annotation. The phylogenetic tree of Tetranychidae was constructed based on 13 protein-coding genes and 2 rRNA gene sequences by maximum likelihood and Bayesian methods. The mitochondrial genomes of E. kankitus on pomelo and tea plants were both in size of 13 032 bp, with GenBank accession numbers OQ955767 and OQ955768, respectively. Both the two mitochondrial genomes included 13 protein coding genes, 2 rRNA genes, 22 tRNA genes and a 40 bp control region, with a consistent gene arrangement. The sequence similarity rate of the two mitochondrial genomes was high, and base composition values of the whole genome and each region were close. E. kankitus on both hosts clustered into one branch in the phylogenetic tree, and the genetic distance between them was 0.068 0, which was much smaller than that between E. kankitus and other mites in Tetranychidae. A close relationship was showed between E. kankitus and Panonychus species in both genetic distance and phylogenetic analysis. According to analyses of mitochondrial genomes, the differentiation degree of E. kankitus on pomelo and tea plants was low, and the risk of transference between the two hosts was high. It is recommended that tea gardens with E. kankitus and surrounding citrus crops should strengthen field monitoring work.

Key words： Eotetranychus kankitus; mitochondrial genome; phylogeny; Tetranychoidae

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