光照和氮素是调控植物能量代谢、碳氮平衡和抗逆性的重要因子。本研究对缺氮处理条件下茶树‘中茗7号’进行暗处理和正常光照处理,发现黑暗处理后茶树叶片变浅,SPAD值显著降低。转录组分析共鉴定得到3 235个差异表达基因（DEGs）,其中1 492个上调,1 743个下调。KEGG富集分析发现DEGs在氮代谢、植物昼夜节律和植物激素信号转导等途径均有明显富集。从DEGs中筛选鉴定得到1个Elongated hypocotyl 5同源基因（CsHY5）。蛋白质互作网络分析表明,CsHY5主要与昼夜节律相关基因互作。通过荧光定量PCR（RT-qPCR）对3种茶树新梢组织中CsHY5的表达水平进行分析。结果表明,‘中茗7号’叶片中CsHY5的表达水平高于大叶种‘云抗10号’和‘红芽佛手’。转录组测序和RT-qPCR分析显示,CsHY5基因在黑暗条件下呈现出明显的下降趋势。上述结果表明,CsHY5可能作为光信号与氮代谢交叉调控的关键节点,在茶树响应暗处理与缺氮双重胁迫过程中发挥重要作用。
Light and nitrogen are crucial factors regulating plant energy metabolism, carbon-nitrogen balance, and stress resistance. In this study, tea plant ‘Zhongming 7’ was subjected to dark treatment and normal light treatment under nitrogen-deficient conditions. The results indicate that dark treatment resulted in a decrease in SPAD values and a noticeable lightening of leaf color. Transcriptome analysis identifies 3 235 differentially expressed genes (DEGs), with 1 492 upregulated and 1 743 downregulated. KEGG enrichment analysis reveals significant enrichment of DEGs in pathways related to nitrogen metabolism, plant circadian rhythms and phytohormone signaling. Among the identified DEGs, an Elongated Hypocotyl 5 (CsHY5) gene was screened for further investigation. Protein-protein interaction network analysis reveals that CsHY5 primarily interacts with circadian rhythm-related genes. The expression level of the CsHY5 gene in new shoot tissues of three tea cultivars was analyzed using quantitative real-time PCR (RT-qPCR). The results reveals that the expression level of CsHY5 gene in the leaves of ‘Zhongming 7’ was higher than those observed in ‘Yunkang 10’ and ‘Hongya Foshou’. Transcriptome sequencing and RT-qPCR analysis indicate that the CsHY5 gene was downregulated under dark treatment. These findings suggest that CsHY5 may serve as a key node in the cross-regulation between light signaling and nitrogen metabolism, playing a crucial role in tea plants' response to combined dark treatment and nitrogen deficiency stress.
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