缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定

doi:10.13305/j.cnki.jts.2026.02.014

茶叶科学 ›› 2026, Vol. 46 ›› Issue (2): 255-263.doi: 10.13305/j.cnki.jts.2026.02.014

缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定

王永鑫¹, 毛卓卓¹, 韦康¹, 王丽鸳¹, 陆文渊^2,*, 余继忠^3,*

1.中国农业科学院茶叶研究所,浙江杭州 310008;
2.湖州市农业科学研究院,浙江湖州 313000;
3.杭州市农业科学研究院茶叶研究所,浙江杭州 310024

收稿日期:2025-12-02 修回日期:2026-01-04 出版日期:2026-04-15 发布日期:2026-04-22
通讯作者: ^*tea66@126.com;hchyu@126.com
作者简介:王永鑫,男,副研究员,主要从事茶树遗传育种方面的研究。
基金资助:
浙江省农业新品种选育重大专项（2021C02067-7-1）; 国家茶叶产业技术体系（CARS-19）; 2024—2026年浙江省产业技术团队项目（生态低碳茶园生产技术集成与应用示范项目）

Transcriptome Analysis of Nitrogen-Deficient Tea Plants Under Dark Treatment and Identification of the CsHY5 Gene

WANG Yongxin¹, MAO Zhuozhuo¹, WEI Kang¹, WANG Liyuan¹, LU Wenyuan^2,*, YU Jizhong^3,*

1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Huzhou Academy of Agricultural Sciences, Huzhou 313000, China;
3. Tea Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2025-12-02 Revised:2026-01-04 Online:2026-04-15 Published:2026-04-22

摘要/Abstract

摘要： 光照和氮素是调控植物能量代谢、碳氮平衡和抗逆性的重要因子。本研究对缺氮处理条件下茶树‘中茗7号’进行暗处理和正常光照处理,发现黑暗处理后茶树叶片变浅,SPAD值显著降低。转录组分析共鉴定得到3 235个差异表达基因（DEGs）,其中1 492个上调,1 743个下调。KEGG富集分析发现DEGs在氮代谢、植物昼夜节律和植物激素信号转导等途径均有明显富集。从DEGs中筛选鉴定得到1个Elongated hypocotyl 5同源基因（CsHY5）。蛋白质互作网络分析表明,CsHY5主要与昼夜节律相关基因互作。通过荧光定量PCR（RT-qPCR）对3种茶树新梢组织中CsHY5的表达水平进行分析。结果表明,‘中茗7号’叶片中CsHY5的表达水平高于大叶种‘云抗10号’和‘红芽佛手’。转录组测序和RT-qPCR分析显示,CsHY5基因在黑暗条件下呈现出明显的下降趋势。上述结果表明,CsHY5可能作为光信号与氮代谢交叉调控的关键节点,在茶树响应暗处理与缺氮双重胁迫过程中发挥重要作用。

关键词: 茶树, CsHY5, 黑暗, 缺氮, 转录组分析

Abstract: Light and nitrogen are crucial factors regulating plant energy metabolism, carbon-nitrogen balance, and stress resistance. In this study, tea plant ‘Zhongming 7’ was subjected to dark treatment and normal light treatment under nitrogen-deficient conditions. The results indicate that dark treatment resulted in a decrease in SPAD values and a noticeable lightening of leaf color. Transcriptome analysis identifies 3 235 differentially expressed genes (DEGs), with 1 492 upregulated and 1 743 downregulated. KEGG enrichment analysis reveals significant enrichment of DEGs in pathways related to nitrogen metabolism, plant circadian rhythms and phytohormone signaling. Among the identified DEGs, an Elongated Hypocotyl 5 (CsHY5) gene was screened for further investigation. Protein-protein interaction network analysis reveals that CsHY5 primarily interacts with circadian rhythm-related genes. The expression level of the CsHY5 gene in new shoot tissues of three tea cultivars was analyzed using quantitative real-time PCR (RT-qPCR). The results reveals that the expression level of CsHY5 gene in the leaves of ‘Zhongming 7’ was higher than those observed in ‘Yunkang 10’ and ‘Hongya Foshou’. Transcriptome sequencing and RT-qPCR analysis indicate that the CsHY5 gene was downregulated under dark treatment. These findings suggest that CsHY5 may serve as a key node in the cross-regulation between light signaling and nitrogen metabolism, playing a crucial role in tea plants' response to combined dark treatment and nitrogen deficiency stress.

Key words: tea plant, CsHY5, darkness, nitrogen deficiency, transcriptome analysis

中图分类号:

S571.1
S326

王永鑫, 毛卓卓, 韦康, 王丽鸳, 陆文渊, 余继忠. 缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定[J]. 茶叶科学, 2026, 46(2): 255-263. doi: 10.13305/j.cnki.jts.2026.02.014.

WANG Yongxin, MAO Zhuozhuo, WEI Kang, WANG Liyuan, LU Wenyuan, YU Jizhong. Transcriptome Analysis of Nitrogen-Deficient Tea Plants Under Dark Treatment and Identification of the CsHY5 Gene[J]. Journal of Tea Science, 2026, 46(2): 255-263. doi: 10.13305/j.cnki.jts.2026.02.014.

参考文献

[1] Zhang Q F, Shi Y T, Hu H, et al.Magnesium promotes tea plant growth via enhanced glutamine synthetase-mediated nitrogen assimilation[J]. Plant Physiology, 2023, 192(2): 1321-1337.
[2] Wang Y X, Lei Y P, Wei K, et al.The effect of hypoxic stress on nitrate absorption and amino acid metabolism of tea plants[J]. Journal of Plant Growth Regulation, 2025, 44(11): 6467-6478.
[3] Zhang Y G, Ye X S, Zhang X W, et al.Natural variations and dynamic changes of nitrogen indices throughout growing seasons for twenty tea plant (Camellia sinensis L.) varieties[J]. Plants, 2020, 9(10): 1333. doi: 10.3390/plants9101333.
[4] Luo Q T, He H F.Accumulation of theanine in tea plant (Camellia sinensis (L.) O. Kuntze): biosynthesis, transportation and strategy for improvement[J]. Plant Science, 2025, 352: 112406. doi: 10.1016/j.plantsci.2025.112406.
[5] Chen Y Z, Wang F, Wu Z D, et al.Effects of long-term nitrogen fertilization on the formation of metabolites related to tea quality in subtropical China[J]. Metabolites, 2021, 11(3): 146. doi: 10.3390/metabo11030146.
[6] Wang Y, Wang Y M, Lu Y T, et al.Influence of different nitrogen sources on carbon and nitrogen metabolism and gene expression in tea plants (Camellia sinensis L.)[J]. Plant Physiology and Biochemistry, 2021, 167(6): 561-566.
[7] Shi H, Lü M, Luo Y W, et al.Genome-wide regulation of light-controlled seedling morphogenesis by three families of transcription factors[J]. PNAS, 2018, 115(25): 6482-6487.
[8] Ponnu J, Hoecker U.Illuminating the COP1/SPA ubiquitin ligase: fresh insights into its structure and functions during plant photomorphogenesis[J]. Fronts Plant Science, 2021, 12: 662793. doi: 10.3389/fpls.2021.662793.
[9] 李桂楠, 杨妮, 罗微, 等. CsDET2基因的鉴定及其对茶树光周期与非生物胁迫的响应分析[J]. 茶叶科学, 2025, 45(5): 742-756.
Li G N, Yang N, Luo W, et al.Identification of CsDET2 and its response analysis to photoperiod and abiotic stress in Camellia sinensis[J]. Journal of Tea Science, 2025, 45(5): 742-756.
[10] Wu Q J, Chen Z D, Sun W J, et al.De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan[J]. Fronts in Plant Science, 2016, 7: 662793. doi: 10.3389/fpls.2021.662793.
[11] Guan C L, Zhang D, Chu C C.Interplay of light and nitrogen for plant growth and development[J]. Crop Journal, 2025, 13(3): 641-655.
[12] Oliveira I C, Coruzzi G M.Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis[J]. Plant Physiology, 1999, 121(1): 301-309.
[13] Jonassen E M, Lea U S, Lillo C.HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage plants[J]. Planta, 2007, 227(3): 559-564.
[14] Gangappa S N, Botto J F.The multifaceted roles of HY5 in plant growth and development[J]. Molecular Plant, 2016, 9(10): 1353-1365.
[15] Stawska M, Oracz K.PhyB and HY5 are involved in the blue light-mediated alleviation of dormancy of seeds possibly via the modulation of expression of genes related to light, GA, and ABA[J]. International Journal of Molecular Sciences, 2019, 20(23): 5882. doi: 10.3390/ijms20235882.
[16] An J, Qu F, Yao J, et al.The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple[J]. Horticulture Research, 2017, 4: 17023. doi: 10.1038/hortres.2017.23.
[17] Ruan J Y, Gerendás J, Härdter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[18] Lillo C.Signalling cascades integrating light-enhanced nitrate metabolism[J]. Biochemical Journal, 2008, 415(1): 11-19.
[19] Jong F D, Thodey K, Lejay L V, et al.Glucose elevates NITRATE TRANSPORTER2.1 protein levels and nitrate transport activity independently of its HEXOKINASE1-MEDIATED stimulation of NITRATE TRANSPORTER2.1 expression[J]. Plant Physiology, 2013, 164(1): 308-320.
[20] 刘海光, 罗振, 董合忠. 植物硝态氮吸收和转运的调控研究进展[J]. 生物技术通报, 2021, 37(6): 192-201.
Liu H G, Luo Z, Dong H Z.Research progress on the regulation of NO₃- uptake and transport in plant[J]. Biotechnology Bulletin, 2021, 37(6): 192-201.
[21] Mu X H, Chen Y L.The physiological response of photosynthesis to nitrogen deficiency[J]. Plant Physiology and Biochemistry, 2021, 158: 76-82. doi: 10.1016/j.plaphy.2020.11.019.
[22] Chen X B, Yao Q F, Gao X H, et al.Shoot-to-root mobile transcription factor HY5 coordinates plant carbon and nitrogen acquisition[J]. Current Biology, 2016, 26(5): 640-646.
[23] Castillon A, Shen H, Huq E.Phytochrome interacting factors: central players in phytochrome-mediated light signaling networks[J]. Trends in Plant Science, 2007, 12(11): 514-521.
[24] Wang Q, Lin C T.Photoreceptor signaling: when COP1 meets VPs[J]. Embo Journal, 2019, 38(18): e102962. doi: 10.15252/embj.2019102962.
[25] Chakraborty M, Gangappa S N, Maurya J P, et al.Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development[J]. Plant Journal, 2019, 99(6): 1080-1097.
[26] Li J, Terzaghi W, Gong Y Y, et al.Modulation of BIN2 kinase activity by HY5 controls hypocotyl elongation in the light[J]. Nature Communications, 2020, 11(1): 1592. doi: 10.1038/s41467-020-15394-7.
[27] Yang G Q, Zhang C L, Dong H X, et al.Activation and negative feedback regulation of transcription by the SlBBX20/21-SlHY5 transcription factor module in UV-B signaling[J]. Plant Cell, 2022, 34(5): 2038-2055.
[28] Liu S Y, Wang Q L, Zhong M, et al.The CRY1-COP1-HY5 axis mediates blue-light regulation of Arabidopsis thermotolerance[J]. Plant Communications, 2025, 6(4): 101264. doi: 10.1016/j.xplc.2025.101264.

缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定

Transcriptome Analysis of Nitrogen-Deficient Tea Plants Under Dark Treatment and Identification of the CsHY5 Gene

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	姜燕华, 何梦迪, 胡桐, 韩震, 陈映男, 吴立赟, 韦康, 王丽鸳. ‘望海茶1号’配套扦插繁育技术和不定根转录特性分析[J]. 茶叶科学, 2026, 46(2): 207-220.
[2]	陈致印, 曾文娟, 杨盼, 文聪, 江睿, 钟倩怡, 敬媛蓉, 朱赞江. ‘槠叶齐’茶树线粒体基因组特征及密码子使用偏好性分析[J]. 茶叶科学, 2026, 46(2): 221-237.
[3]	夏露霞, 韩善捷, 韩宝瑜, 王梦馨. 3个黄化茶树品种对小贯小绿叶蝉抗性机制的比较研究[J]. 茶叶科学, 2026, 46(2): 238-254.
[4]	黎健龙, 廖茵茵, 陈家铭, 张曼, 曾兰亭, 农红秋, 唐劲驰. 修剪对涝渍茶树生长及养分吸收的影响研究[J]. 茶叶科学, 2026, 46(2): 264-278.
[5]	叶诚诚, 吴秀云, 李紫成, 吴碎典, 金山发, 朱洁. 病害侵染下茶树叶际微生物的响应[J]. 茶叶科学, 2026, 46(2): 279-291.
[6]	金怀, 崔婷, 王楠, 魏丽萍, 张荣, 巩文峰. 西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性[J]. 茶叶科学, 2026, 46(2): 318-330.
[7]	刘恩贝, 吴叶蝶, 徐苗苗, 凌名杏, 彭靖, 王洁, 王新超, 王璐. 茶树WCOR413基因家族鉴定与表达调控分析[J]. 茶叶科学, 2026, 46(1): 1-19.
[8]	周慧, 崔悠, 陈雯剑, 杜悦阳, 张欢, 苏鸿锋, 张凯凯, 张凌云. 转录因子CsMYB75-like-2调控茶树花青素合成的分子机制研究[J]. 茶叶科学, 2026, 46(1): 20-34.
[9]	钟丽凡, 胡芸青, 周秦羽, 杨旺, 王利, 刘振. 基于农艺性状与全基因组测序的湖南碣滩茶遗传多样性分析及核心种质构建[J]. 茶叶科学, 2026, 46(1): 35-49.
[10]	张云帆, 周凤珏, 胡钧铭, 宋传奎, 郑富海, 张俊辉, 李婷婷, 李宇翔. 等离子活化乳酸钠增强幼龄茶树次生代谢及生理抗性[J]. 茶叶科学, 2026, 46(1): 61-72.
[11]	张倩, 刘盼盼, 何碧云, 王治会, 吴伟伟, 高晨曦, 曾雯, 孙威江. 丛枝菌根真菌对茶树鲜叶理化成分及成品茶品质的影响[J]. 茶叶科学, 2026, 46(1): 73-88.
[12]	马思宇, 龙丽雪, 李子龙, 赵显汪, 何鹏飞, 何鹏搏, 陈林波, 曲浩, 龙亚芹, 唐萍. 云南大叶种茶树炭疽病病原分离鉴定及生防菌筛选[J]. 茶叶科学, 2026, 46(1): 89-100.
[13]	秦道正, 张婷玉, 谢康雯, 毕建宇, 张欢, 方微, 徐业. 陕西茶区叶蝉及蜡蝉类害虫学名厘定及中国茶树小绿叶蝉学名更正[J]. 茶叶科学, 2026, 46(1): 101-110.
[14]	任宁, 薛向磊, 郑航, 沈帅, 俞国红. 纯电动悬臂茶树修剪机的设计与试验[J]. 茶叶科学, 2026, 46(1): 111-121.
[15]	厉媛媛, 姚明哲, 金基强. 茶树特异种质资源高EGCG3′′Me性状形成的分子机制解析[J]. 茶叶科学, 2025, 45(6): 909-919.