‘望海茶1号’配套扦插繁育技术和不定根转录特性分析

doi:10.13305/j.cnki.jts.2026.02.003

茶叶科学 ›› 2026, Vol. 46 ›› Issue (2): 207-220.doi: 10.13305/j.cnki.jts.2026.02.003

‘望海茶1号’配套扦插繁育技术和不定根转录特性分析

姜燕华², 何梦迪¹, 胡桐², 韩震⁴, 陈映男³, 吴立赟¹, 韦康¹, 王丽鸳^1,*

1.中国农业科学院茶叶研究所/国家茶树改良中心,浙江杭州 310008;
2.宁海县农业产业化发展中心,浙江宁海 315600;
3.宁海县农业农村局,浙江宁海 315600;
4.宁波市农业技术推广总站,浙江宁波 315012

收稿日期:2025-06-30 修回日期:2025-07-30 出版日期:2026-04-15 发布日期:2026-04-22
通讯作者: ^*wangly@tricaas.com
作者简介:姜燕华,女,高级农艺师,从事茶叶生产技术推广工作,277584820@qq.com。
基金资助:
浙江省农业新品种选育重大专项-茶树（2021C02067-7-1）; 国家茶叶产业技术体系（CARS-19）

Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for ‘Wanghaicha 1’

JIANG Yanhua², HE Mengdi¹, HU Tong², HAN Zhen⁴, CHEN Yingnan³, WU Liyun¹, WEI Kang¹, WANG Liyuan^1,*

1. National Centre for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Agricultural Industrialization Development Center of Ninghai County, Ninghai 315600, China;
3. Bureau of Agricultural and Rural Affairs of Ninghai County, Ninghai 315600, China;
4. Ningbo Agricultural Technology Extension Station of Ningbo City, Ningbo 315012, China

Received:2025-06-30 Revised:2025-07-30 Online:2026-04-15 Published:2026-04-22

摘要/Abstract

摘要： ‘望海茶1号’是从宁海县地方群体种中系统选育而成的茶树新品种。为建立高效的繁育体系,在嵊州试验基地开展轻基质无纺布育苗袋长短穗对比扦插试验和短穗田间扦插覆地膜试验。结果表明,‘望海茶1号’基质短穗扦插苗发根速率高于对照‘龙井43’,长势优,更耐高温和干旱;基质长枝扦插苗77 d可达到出圃标准;在苗床上覆盖黑色无纺布膜的短穗田间扦插方法能有效减少6次人工除草。通过Illumina HiSeq平台进行‘望海茶1号’根系转录组测序,与对照‘龙井43’相比,筛选到植物激素相关差异表达基因107个,推测这些基因的协同调控可能是该品种根系发达的分子基础。初步在‘望海茶1号’不定根中筛选到107个与植物激素相关的差异表达基因,并建立了“长枝早出圃+覆黑地膜提质增效”的扦插模式,可缩短15.4%育苗周期并降低85.7%除草成本,为新品种产业化推广提供理论依据和技术支撑。

关键词: 茶树新品种, 育种, 扦插技术, 不定根, 转录组

Abstract: ‘Wanghaicha 1’ is a newly bred green tea cultivar origining from the local population in Ninghai county. To establish an efficient breeding system, a comparative cutting experiment of long and short branch cuttings in lightweight non-woven seedling bags, and a short branch field mulching cutting experiment were set up at the Shengzhou base. The results show that the rooting rate of short branch cuttings of this cultivar was higher than that of the control ‘Longjing 43’, with better growth and greater tolerance to high temperatures and drought. Moreover, the long branch cuttings can reach the nursery standard after 77 days. The method of short branch cuttings covered with black non-woven fabric film on the seedbed can effectively reduce the frequency of manual weeding. And through Illumina HiSeq platform, transcriptome sequencing of the root system of ‘Wanghaicha1’ was performed. Compared with the control ‘Longjing 43’, 107 differentially expressed genes related to plant hormones were screened. It is speculated that the synergistic regulation of these genes may be the molecular basis for the developed root system of this culitvar. This study preliminarily obtained 107 differentially expressed genes related to plant hormones in the adventitious roots of ‘Wanghaicha1’. The established cutting mode of ‘early emergence of long branch from the nursery application of black plastic film to improve quality and efficiency’ can shorten the seedling period by 15.4% and reduce weed control costs by 85.7%, providing theoretical basis and technical support for the industrial promotion of new cultivar.

Key words: new tea cultivar, breeding, cutting techniques, adventitious root, transcriptome

中图分类号:

姜燕华, 何梦迪, 胡桐, 韩震, 陈映男, 吴立赟, 韦康, 王丽鸳. ‘望海茶1号’配套扦插繁育技术和不定根转录特性分析[J]. 茶叶科学, 2026, 46(2): 207-220. doi: 10.13305/j.cnki.jts.2026.02.003.

JIANG Yanhua, HE Mengdi, HU Tong, HAN Zhen, CHEN Yingnan, WU Liyun, WEI Kang, WANG Liyuan. Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for ‘Wanghaicha 1’[J]. Journal of Tea Science, 2026, 46(2): 207-220. doi: 10.13305/j.cnki.jts.2026.02.003.

参考文献

[1] 江用文, 陈霄雄, 朱建淼, 等. 中国茶产业2020年发展规模分析[J]. 茶叶科学, 2011, 31(3): 273-282.
Jiang Y W, Chen X X, Zhu J M, et al.Analysis on development scale of Chinese tea industry in 2020[J]. Journal of Tea Science, 2011, 31(3): 273-282.
[2] 王新超, 王璐, 郝心愿, 等. 茶树遗传育种研究“十三五”进展及“十四五”发展方向[J]. 中国茶叶, 2021, 43(9): 50-57.
Wang X C, Wang L, Hao X Y, et al.Tea genetics and breeding progress during the 13th Five-Year Plan period and development direction in the 14th Five-Year Plan period[J]. China Tea, 2021, 43(9): 50-57.
[3] 李兰英, 刘东娜, 龚雪蛟, 等. 茶树新品种‘甘露1号’选育研究[J]. 茶叶学报, 2023, 64(2): 22-28.
Li L Y, Liu D N, Gong X J, et al.Breeding new tea variety, Ganlu No. 1[J]. Acta Tea Sinica, 2023, 64(2): 22-28.
[4] 姜燕华, 王丽鸳, 成浩, 等. 茶树望海茶1号生态适应性及其制茶品质[J]. 浙江农业科学, 2019, 60(10): 1791-1792.
Jiang Y H, Wang L Y, Cheng H, et al.Ecological adaptability and tea making quality of tea tree No.1 of Wanghai tea[J]. Journal of Zhejiang Agricultural Sciences, 2019, 60(10): 1791-1792.
[5] 张宏, 金洁, 王剑峰. 生长素在微生物调控根发育过程中的作用[J]. 西北植物学报, 2018, 38(7): 1369-1374.
Zhang H, Jin J, Wang J F.Role of auxin in the process of microbe control of root development[J]. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(7): 1369-1374.
[6] Willige B C, Isono E, Richter R, et al.Gibberellin regulates PIN-FORMED abundance and is required for auxin transport-dependent growth and development in Arabidopsis thaliana[J]. The Plant Cell, 2011, 23(6): 2184-2195.
[7] Wang S T, Wu H R, Zhang Y Z, et al.Transcriptome reveals the regulation of exogenous auxin inducing rooting of non-rooting callus of tea cuttings[J]. International Journal of Molecular Sciences, 2024, 25(15): 8080. doi: 10.3390/ijms25158080.
[8] Liu M, Zhang H H, Fang X Z, et al.Auxin acts downstream of ethylene and nitric oxide to regulate magnesium deficiency-induced root hair development in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2018, 59(7): 1452-1465.
[9] Li H, Teng R M, Liu J X, et al.Identification and analysis of genes involved in auxin, abscisic acid, gibberellin, and brassinosteroid metabolisms under drought stress in tender shoots of tea plants[J]. DNA and Cell Biology, 2019, 38(11): 1292-1302.
[10] 岳川, 曾建明, 章志芳, 等. 茶树中植物激素研究进展[J]. 茶叶科学, 2012, 32(5): 382-392.
Yue C, Zeng J M, Zhang Z F, et al.Research progress in the phytohormone of tea plant (Camellia sinensis)[J]. Journal of Tea Science, 2012, 32(5): 382-392.
[11] 崔思宇, 张志芬, 付晓峰, 等. 燕麦植物激素信号转导通路中响应干旱胁迫的关键基因[J]. 麦类作物学报, 2023, 43(11): 1384-1393.
Cui S Y, Zhang Z F, Fu X F, et al.Key genesin response to drought stress in plant hormone signal transduction pathway of oat[J]. Journal of Triticeae Crops, 2023, 43(11): 1384-1393.
[12] 唐子贻, 杜玥, 杨宏斌, 等. 高温和干旱胁迫下茶树叶片内源激素含量变化及其相关基因的表达分析[J]. 茶叶科学, 2023, 43(4): 489-500.
Tang Z Y, Du Y, Yang H B, et al.Changes of endogenous hormone contents and expression analysis of related genes in leaves of tea plants under heat and drought stresses[J]. Journal of Tea Science, 2023, 43(4): 489-500.
[13] 陈强. 金观音扦插育苗关键因子分析[J]. 福建茶叶, 2022, 44(12): 13-15.
Chen Q.Analysis of key factors in cutting seedling cultivation of Jinguanyin[J]. Tea in Fujian, 2022, 44(12): 13-15.
[14] 邱勇娟, 蓝燕, 赖兆荣. 新育成茶树品种扦插试验研究[J]. 中国农村小康科技, 2010(8): 50-51.
Qiu Y J, Lan Y, Lai Z R.Cutting experimental research of new cultivated tea tree breeds[J]. Chinese Countryside Well-off Technology, 2010(8): 50-51.
[15] 贾志远, 葛晓敏, 唐罗忠. 木本植物扦插繁殖及其影响因素[J]. 世界林业研究, 2015, 28(2): 36-41.
Jia Z Y, Ge X M, Tang L Z.Research progress in cutting propagation technology for woody plants and its affecting factors[J]. World Forestry Research, 2015, 28(2): 36-41.
[16] 王小玲, 赵忠, 高柱, 等. 插壤温度对四倍体刺槐硬枝插条生根过程中生理生化的影响[J]. 植物生理学报, 2012, 48(7): 699-704.
Wang X L, Zhao Z, Gao Z, et al.Effects of plug soil temperature on physiological and biochemical in hard- wood cuttings rooting of tetraploid Robinia pseudoacacia[J]. Plant Physiology Journal, 2012, 48(7): 699-704.
[17] Nie J, Wen C, Xi L, et al.The AP2/ERF transcription factor CmERF053 of chrysanthemum positively regulates shoot branching, lateral root, and drought tolerance[J]. Plant Cell Reports, 2018, 37(7): 1049-1060.
[18] Dubois M, Broeck D V L, Inzé D, et al. The pivotal role of ethylene in plant growth[J]. Trends in Plant Science, 2018, 23(4): 311-323.
[19] 刘志薇, 熊洋洋, 李彤, 等. 茶树转录因子基因CsDREB-A4的克隆与温度胁迫响应的分析[J]. 茶叶科学, 2015, 35(1): 24-34.
Liu Z W, Xiong Y Y, Li T, et al.The cloning of transcription factor gene CsDREB-A4 and the response to temperature stress in Camellia sinensis[J]. Journal of Tea Science, 2015, 35(1): 24-34.
[20] Wang H Y, Wang H L, Shao H B, et al.Recent advances in utilizing transcription factors to improve plant abiotic stress tolerance by transgenic technology[J]. Frontiers in Plant Science, 2016, 7: 67. doi: 10.3389/fpls.2016.00067.
[21] Liu H C, Charng Y Y.Acquired thermotolerance independent of heat shock factor A1 (HsfA1) the master regulator of the heat stress response[J]. Plant Signaling & Behavior, 2012, 7(5): 547-550.
[22] 高莉娟. 紫象草对外施植物生长调节剂的转录响应及bHLH基因家族分析[D]. 兰州: 兰州大学, 2021.
Gao L J.Study on transcript responses of exogenous plant growth regulators treatment and analysis of the bHLH gene family in Cenchrus purpureus Schumach cv. Purple [D]. Lanzhou: Lanzhou University, 2021.
[23] 刘晓月. 水稻转录因子抗逆表达谱及功能验证分析[D]. 北京: 中国农业科学院, 2013.
Liu X Y.Expression and function analysis of transcription factors in rice under abiotic stress [D]. Beijing: Chinese Academy of Agricultural Sciences, 2013.
[24] Bouzroud S, Gasparini K, Hu G, et al.Down regulation and loss of auxin response factor 4 function using CRISPR/Cas9 alters plant growth, stomatal function and improves tomato tolerance to salinity[J]. Genes, 2020, 11(3): 272. doi: 10.3390/genes11030272.
[25] Zhu H, Zhou Y Y, Zhai H, et al.Transcriptome profiling reveals insights into the molecular mechanism of drought tolerance in sweetpotato[J]. Journal of Integrative Agriculture, 2019, 18(1): 9-23.
[26] 胡雅丹, 伍国强, 刘晨, 等. MYB转录因子在调控植物响应逆境胁迫中的作用[J]. 生物技术通报, 2024, 40(6): 5-22.
Hu Y D, Wu G Q, Liu C, et al.Roles of MYB transcription factor in regulating the responses of plants to stress[J]. Biotechnology Bulletin, 2024, 40(6): 5-22.
[27] Xie Z Z, Jin L, Sun Y, et al.OsNAC120 balances plant growth and drought tolerance by integrating GA and ABA signaling in rice[J]. Plant Communications, 2024, 5(3): 100782. doi: 10.1016/j.xplc.2023.100782.

‘望海茶1号’配套扦插繁育技术和不定根转录特性分析

Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for ‘Wanghaicha 1’

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	王永鑫, 毛卓卓, 韦康, 王丽鸳, 陆文渊, 余继忠. 缺氮茶树暗处理下转录组分析及CsHY5基因的鉴定[J]. 茶叶科学, 2026, 46(2): 255-263.
[2]	徐歆, 李亚奇, 杨亦扬, 徐琪, 钱雪飞, 马春雷, 梅菊芬. AI茶树育种技术：以黄化性状预测为例[J]. 茶叶科学, 2025, 45(3): 393-401.
[3]	翟秀明, 李解, 肖富良, 唐敏, 曾乐武, 侯渝嘉, 汤燚. 茶树茎叶并联变异差异表达基因的WGCNA分析[J]. 茶叶科学, 2025, 45(3): 402-414.
[4]	刘晓璐, 朱亚兰, 于敏, 盖新月, 范延艮, 孙平, 黄晓琴. 低温胁迫下茶树叶片细胞壁结构变化及光合特性[J]. 茶叶科学, 2024, 44(6): 917-927.
[5]	丁树洽, 谢昕雅, 刘助生, 廖贤军, 刘仲华, 蔡淑娴. 茶叶成分EGCG与L-theanine联合应用的神经保护作用研究[J]. 茶叶科学, 2024, 44(5): 779-792.
[6]	王冬雪, 满佳旭, 武思敏, 赵雪婷, 张冬英. 云南十里香茶树空间转录组测序研究[J]. 茶叶科学, 2024, 44(3): 399-410.
[7]	黄梦迪, 陈兰, 苏芹, 胡锦瑜, 刘桂芝, 谭月萍, 刘硕谦, 田娜. 茶树春梢萌发早晚关联基因CsAL1的CAPS分子标记开发[J]. 茶叶科学, 2024, 44(2): 207-218.
[8]	曾鸿哲, 彭丽媛, 万丽玮, 刘昌伟, 方雯雯, 王阔飞, 张欣仪, 文帅, 黄建安, 刘仲华. 基于肝脏转录组学探究发花红茶降血糖作用的潜在机制[J]. 茶叶科学, 2023, 43(5): 645-656.
[9]	李聪聪, 王浩乾, 叶玙璠, 陈瑶, 任恒泽, 李宇腾, 郝心愿, 王新超, 曹红利, 岳川. 植物激素对茶树春季新梢生长发育的调控作用研究[J]. 茶叶科学, 2023, 43(3): 335-348.
[10]	汤榕津, 刘浩然, 刘丁丁, 张晨禹, 龚洋, 叶圆圆, 陈杰丹, 陈亮, 马春雷. 茶树白化果皮超微结构观察及分子机制研究[J]. 茶叶科学, 2022, 42(6): 779-790.
[11]	龙亚芹, 罗梓文, 王雪松, 龙丽雪, 玉香甩, 李金龙, 曲浩, 汪云刚, 陈林波. 茶谷蛾成虫触角转录组及嗅觉相关基因分析[J]. 茶叶科学, 2021, 41(4): 553-563.
[12]	刘丁丁, 王君雅, 汤榕津, 陈亮, 马春雷. 茶树PPR基因家族全基因组鉴定及白化相关基因表达分析[J]. 茶叶科学, 2021, 41(2): 159-172.
[13]	孙云南, 许燕, 冉隆珣, 蒋会兵, 宋维希, 夏丽飞, 陈林波, 梁名志. 茶树叶片响应茶饼病侵染的转录组分析[J]. 茶叶科学, 2020, 40(1): 113-124.
[14]	王君雅, 陈玮, 刘丁丁, 陈亮, 姚明哲, 马春雷. 不同品种茶树新梢响应“倒春寒”的转录组分析[J]. 茶叶科学, 2019, 39(2): 181-192.
[15]	向奕, 刘硕谦, 龚志华, 陈栋, 肖文军. 茶树紫芽中花青素形成相关基因差异表达分析[J]. 茶叶科学, 2018, 38(5): 439-449.