&#x02018;望海茶1号&#x02019;配套扦插繁育技术和不定根转录特性分析

姜燕华; 何梦迪; 胡桐; 韩震; 陈映男; 吴立赟; 韦康; 王丽鸳

doi:10.13305/j.cnki.jts.2026.02.003

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茶叶科学 ›› 2026, Vol. 46 ›› Issue (2) : 207-220. DOI: 10.13305/j.cnki.jts.2026.02.003

研究报告

‘望海茶1号’配套扦插繁育技术和不定根转录特性分析

姜燕华², 何梦迪¹, 胡桐², 韩震⁴, 陈映男³, 吴立赟¹, 韦康¹, 王丽鸳^1,*

作者信息 +

Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for ‘Wanghaicha 1’

JIANG Yanhua², HE Mengdi¹, HU Tong², HAN Zhen⁴, CHEN Yingnan³, WU Liyun¹, WEI Kang¹, WANG Liyuan^1,*

Author information +

文章历史 +

摘要

‘望海茶1号’是从宁海县地方群体种中系统选育而成的茶树新品种。为建立高效的繁育体系,在嵊州试验基地开展轻基质无纺布育苗袋长短穗对比扦插试验和短穗田间扦插覆地膜试验。结果表明,‘望海茶1号’基质短穗扦插苗发根速率高于对照‘龙井43’,长势优,更耐高温和干旱;基质长枝扦插苗77 d可达到出圃标准;在苗床上覆盖黑色无纺布膜的短穗田间扦插方法能有效减少6次人工除草。通过Illumina HiSeq平台进行‘望海茶1号’根系转录组测序,与对照‘龙井43’相比,筛选到植物激素相关差异表达基因107个,推测这些基因的协同调控可能是该品种根系发达的分子基础。初步在‘望海茶1号’不定根中筛选到107个与植物激素相关的差异表达基因,并建立了“长枝早出圃+覆黑地膜提质增效”的扦插模式,可缩短15.4%育苗周期并降低85.7%除草成本,为新品种产业化推广提供理论依据和技术支撑。

Abstract

‘Wanghaicha 1’ is a newly bred green tea cultivar origining from the local population in Ninghai county. To establish an efficient breeding system, a comparative cutting experiment of long and short branch cuttings in lightweight non-woven seedling bags, and a short branch field mulching cutting experiment were set up at the Shengzhou base. The results show that the rooting rate of short branch cuttings of this cultivar was higher than that of the control ‘Longjing 43’, with better growth and greater tolerance to high temperatures and drought. Moreover, the long branch cuttings can reach the nursery standard after 77 days. The method of short branch cuttings covered with black non-woven fabric film on the seedbed can effectively reduce the frequency of manual weeding. And through Illumina HiSeq platform, transcriptome sequencing of the root system of ‘Wanghaicha1’ was performed. Compared with the control ‘Longjing 43’, 107 differentially expressed genes related to plant hormones were screened. It is speculated that the synergistic regulation of these genes may be the molecular basis for the developed root system of this culitvar. This study preliminarily obtained 107 differentially expressed genes related to plant hormones in the adventitious roots of ‘Wanghaicha1’. The established cutting mode of ‘early emergence of long branch from the nursery application of black plastic film to improve quality and efficiency’ can shorten the seedling period by 15.4% and reduce weed control costs by 85.7%, providing theoretical basis and technical support for the industrial promotion of new cultivar.

导出引用

姜燕华, 何梦迪, 胡桐, 韩震, 陈映男, 吴立赟, 韦康, 王丽鸳. ‘望海茶1号’配套扦插繁育技术和不定根转录特性分析[J]. 茶叶科学. 2026, 46(2): 207-220 https://doi.org/10.13305/j.cnki.jts.2026.02.003

JIANG Yanhua, HE Mengdi, HU Tong, HAN Zhen, CHEN Yingnan, WU Liyun, WEI Kang, WANG Liyuan. Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for ‘Wanghaicha 1’[J]. Journal of Tea Science. 2026, 46(2): 207-220 https://doi.org/10.13305/j.cnki.jts.2026.02.003

中图分类号： S571.1 S339.4

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