茶叶成分EGCG与L-theanine联合应用的神经保护作用研究

doi:10.13305/j.cnki.jts.20240918.001

摘要/Abstract

摘要： 分化的神经细胞需要在细胞静息状态下维护轴突的生长和功能。前期研究显示,表没食子儿茶素没食子酸酯（Epigallocatechin gallate,EGCG）和L-茶氨酸（L-theanine）能够维持神经细胞的静息状态并具有神经修复作用,但具体的作用机制还不清楚。在Aβ_25-35诱导的PC12细胞损伤模型中,EGCG和L-theanine联合处理改善细胞代谢和修复能力,提高了细胞活力,表现显著的协同作用。转录组和网络药理学分析结果表明,EGCG主要通过抑制氧化应激、调节脂肪酸代谢和淀粉样蛋白毒性应激来维持细胞静息态;L-theanine则通过促进轴突生长、调节神经代谢和突触功能发挥作用。两者联合应用对细胞网络的调节更为广泛和温和,减少对细胞的刺激作用。本研究为茶叶的神经保护作用及其在老龄化社会中的饮用价值提供了理论依据。

关键词: 表没食子儿茶素没食子酸酯, L-茶氨酸, 神经保护, 网络药理, 转录组, 联合分析

Abstract: Differentiated neurons need to maintain axonal growth and function in a quiescent state. Previous studies have shown that epigallocatechin gallate (EGCG) and L-theanine can maintain the quiescent state of neurons and have neurorestorative effects, although the specific mechanisms are still unclear. In the Aβ_25-35-induced PC12 cell damage model, combined treatment with EGCG and L-theanine improved cell metabolism and repair capacity, enhanced cell viability and showed a significant synergistic effect. Transcriptomic and network pharmacological analyses indicate that EGCG mainly maintains the quiescent state of cells by inhibiting oxidative stress, regulating fatty acid metabolism, and mitigating amyloid protein toxicity stress. L-theanine promotes axonal growth and regulates neuronal metabolism and synaptic function. The combined application of both compounds results in a broader and milder regulation of cellular networks, reducing cellular stress. This study provided theoretical support for the neuroprotective effects of tea and its value in an aging society.

Key words: EGCG, L-theanine, neuroprotection, network pharmacology, transcriptome, joint analysis

中图分类号:

丁树洽, 谢昕雅, 刘助生, 廖贤军, 刘仲华, 蔡淑娴. 茶叶成分EGCG与L-theanine联合应用的神经保护作用研究[J]. 茶叶科学, 2024, 44(5): 779-792. doi: 10.13305/j.cnki.jts.20240918.001.

DING Shuqia, XIE Xinya, LIU Zhusheng, LIAO Xianjun, LIU Zhonghua, CAI Shuxian. A Study on the Neuroprotective Effects of Combined EGCG and L-Theanine from Tea Leaves[J]. Journal of Tea Science, 2024, 44(5): 779-792. doi: 10.13305/j.cnki.jts.20240918.001.

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