表没食子儿茶素没食子酸酯抗汉滩病毒效果研究

doi:10.13305/j.cnki.jts.2025.01.014

摘要/Abstract

摘要： 为明确表没食子儿茶素没食子酸酯（Epigallocatechin gallate,EGCG）在体内外对汉滩病毒（Hantaan virus,HTNV）的抑制成效,开展以下试验：（1）运用细胞活力检测法测定EGCG的细胞毒性;（2）在感染HTNV的不同时段添加EGCG进行处理,采用蛋白免疫印迹、实时荧光定量PCR、间接免疫荧光及斑点形成试验（Focus formation assay,FFA）检测HTNV在A549细胞内24、48 h的病毒表达量,同时使用Autodock vina软件进行分子对接,确定EGCG抗HTNV的潜在作用靶点;（3）通过灌胃给药方式给予攻毒裸鼠高中低剂量的EGCG,并检测其体重及生存率。结果表明,使用100 µmol·L^-1 EGCG体外有效抑制HTNV感染,且该抑制效果主要作用于HTNV的吸附阶段;分子对接试验显示,EGCG可与HTNV的Gn、Gc相互作用,结合能分别为﹣9.0、﹣7.1 kcal·mol^-1;高中低剂量EGCG（50.0、25.0、12.5 mg·kg^-1）治疗感染裸鼠后,有效降低了攻毒裸鼠死亡率,裸鼠体重下降幅度均明显减少。

关键词: 汉滩病毒, 表没食子儿茶素没食子酸酯, 抗病毒感染

Abstract: The purpose of this study was to investigate the inhibitory effect of epigallocatechin gallate (EGCG) on the Hantaan virus (HTNV) both in vitro and in vivo, and to identify the potential targets of EGCG against HTNV using molecular docking. First, the cytotoxicity of EGCG was determined using a cell viability assay. Then, EGCG was administered for treatment at different time points during HTNV infection, and the viral expression of HTNV in A549 cells was detected by western blotting, real-time PCR, indirect immunofluorescence, and focus formation assay (FFA) at 24 h and 48 h. Molecular docking was conducted using Autodock Vina software. Finally, the challenged nude mice were administered high, medium, and low doses of EGCG via gavage, and their body weight and survival rates were measured. The results indicate that the administration of 100 μmol·L^-1 EGCG effectively inhibited HTNV infection in vitro, primarily affecting the adsorption stage of HTNV. The molecular docking results demonstrate that EGCG could interact with HTNV Gn and Gc, with binding energies of ﹣9.0 kcal·mol^-1 and ﹣7.1 kcal·mol^-1, respectively. High, medium, and low doses of EGCG (50.0, 25.0, 12.5 mg·kg^-1) effectively reduced the mortality rate of challenged nude mice, and significantly mitigated weight loss in these mice.

Key words: HTNV, EGCG, antiviral infection

中图分类号:

罗璐璐, 赵悦汐, 王彦博, 幸怡, 齐洋, 马宏炜, 程林峰, 张芳琳. 表没食子儿茶素没食子酸酯抗汉滩病毒效果研究[J]. 茶叶科学, 2025, 45(1): 145-156. doi: 10.13305/j.cnki.jts.2025.01.014.

LUO Lulu, ZHAO Yuexi, WANG Yanbo, XING Yi, QI Yang, MA Hongwei, CHENG Linfeng, ZHANG Fanglin. Study on the Effect of Epigallocatechin Gallate Against Hantaan Virus[J]. Journal of Tea Science, 2025, 45(1): 145-156. doi: 10.13305/j.cnki.jts.2025.01.014.

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