欢迎访问《茶叶科学》,今天是

EGCG对细胞的促氧化作用研究进展

  • 陈怡君 ,
  • 熊立瑰 ,
  • 黄建安 ,
  • 龚雨顺 ,
  • 刘仲华
展开
  • 1. 湖南农业大学茶学教育部重点实验室,湖南农业大学,湖南 长沙 410128;
    2. 国家植物功能成分利用工程技术研究中心,湖南农业大学,湖南 长沙 410128;
    3. 植物功能成分利用协同创新中心,湖南农业大学,湖南 长沙 410128
陈怡君,女,硕士研究生,主要从事茶叶功能成分研究。

收稿日期: 2014-12-18

  修回日期: 2015-02-03

  网络出版日期: 2019-08-23

基金资助

国家自然科学基金(31270733)

Review on Pro-oxidative Properties of EGCG on Cell

  • CHEN Yijun ,
  • XIONG Ligui ,
  • HUANG Jian′an ,
  • GONG Yushun ,
  • LIU Zhonghua
Expand
  • 1. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China;
    3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Hunan Agriculture University, Changsha 410128, China

Received date: 2014-12-18

  Revised date: 2015-02-03

  Online published: 2019-08-23

摘要

EGCG是茶叶中主要的儿茶素类物质,具有多种生物活性。近年来,EGCG促氧化作用得到了广泛关注。本文对国内外细胞实验中EGCG的促氧化作用进行综述。EGCG在细胞培养基中发生自动氧化可以形成细胞外氧化应激环境,这种自动氧化受到培养基种类、EGCG浓度与处理时间、血清含量及pH值等因素影响。在细胞内,EGCG直接提高细胞内活性氧(Reactive Oxidative Species, ROS)和线粒体ROS,或者通过Fenton反应间接产生OH-。EGCG可以影响细胞内转录因子、信号通路与表面生长因子受体的表达与传递,从而影响细胞的一系列生命活动。

本文引用格式

陈怡君 , 熊立瑰 , 黄建安 , 龚雨顺 , 刘仲华 . EGCG对细胞的促氧化作用研究进展[J]. 茶叶科学, 2015 , 35(2) : 130 -136 . DOI: 10.13305/j.cnki.jts.2015.02.004

Abstract

EGCG, which is the most abundant catechin in tea, has been demonstrated to possess a wide range of biochemical and pharmacological activities. Recent investigations have revealed that EGCG functions as a pro-oxidant. In this review, we discussed the pro-oxidative mechanism of EGCG in cell culture. Auto-oxidation of EGCG, influenced by the media types, dose- and time-dependent of EGCG addition, contents of serum and pH value etc., forms environmental oxidative stress in cell medium. Furthermore, EGCG produce intracellular ROS and mtROS, and trigger the Fenton reaction to create OH- indirectly. In particular, pro-oxidation of EGCG regulate intracellular transcription factors, signalling pathways, and cell surface receptor to exert many biological actions in cell.

参考文献

[1] Yang CS, Wang X, Lu G, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature Reviews Cancer, 2009, 9(6): 429-439.
[2] Gordon NC, Wareham DW.Antimicrobial activity of the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) against clinical isolates of Stenotrophomonas maltophilia[J]. International Journal of Antimicrobial Agents, 2010, 36(2): 129-131.
[3] Singh BN, Shankar S, Srivastava RK.Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications[J]. Biochemical Pharmacology, 2011, 82(12): 1807-1821.
[4] Chu KO, Chan SO, Pang CP, et al. Pro-oxidative, anti-oxidative controls and signaling modification of polyphenolic phytochemicals-contribution to health promotion and disease prevention?[J]. Journal of Agricultural and Food Chemistry, 2014, 62(18): 4026-4038.
[5] Na H-K, Surh Y-J.Modulation of Nrf2-mediated antioxidant and detoxifying enzyme induction by the green tea polyphenol EGCG[J]. Food and Chemical Toxicology, 2008, 46(4): 1271-1278.
[6] Coyle CH, Philips BJ, Morrisroe SN, et al. Antioxidant effects of green tea and its polyphenols on bladder cells[J]. Life Sciences, 2008, 83(1): 12-18.
[7] Shi X, Ye J, Leonard S, et al. Antioxidant properties of (-)-epicatechin-3-gallate and its inhibition of Cr (VI)-induced DNA damage and Cr (IV)-or TPA-stimulated NF-κB activation[J]. Molecular and Cellular Biochemistry, 2000, 206(1/2): 125-132.
[8] Ishii T, Mori T, Tanaka T, et al. Covalent modification of proteins by green tea polyphenol (-)-epigallocatechin-3-gallate through autoxidation[J]. Free Radical Biology and Medicine, 2008, 45(10): 1384-1394.
[9] Sang S, Hou Z, Lambert JD, et al. Redox properties of tea polyphenols and related biological activities[J]. Antioxidants & Redox Signaling, 2005, 7(11/12): 1704-1714.
[10] Mochizuki M, Yamazaki S-i, Kano K, et al. Kinetic analysis and mechanistic aspects of autoxidation of catechins[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2002, 1569(1): 35-44.
[11] Chen R, Wang JB, Zhang XQ, et al. Green tea polyphenol epigallocatechin-3-gallate (EGCG) induced intermolecular cross-linking of membrane proteins[J]. Archives of Biochemistry and Biophysics, 2011, 507(2): 343-349.
[12] Hou Z, Sang S, You H, et al. Mechanism of action of (-)-epigallocatechin-3-gallate: auto-oxidation-dependent inactivation of epidermal growth factor receptor and direct effects on growth inhibition in human esophageal cancer KYSE 150 cells[J]. Cancer Research, 2005, 65(17): 8049-8056.
[13] Sang S, Lee MJ, Hou Z, et al. Stability of tea polyphenol (-)-epigallocatechin-3-gallate and formation of dimers and epimers under common experimental conditions[J]. Journal of Agricultural and Food Chemistry, 2005, 53(24): 9478-9484.
[14] Yang CS, Hong J, Hou Z, et al. Green tea polyphenols: antioxidative and prooxidative effects[J]. The Journal of Nutrition, 2004, 134(11): 3181S.
[15] Hong J, Lu H, Meng X, et al. Stability, cellular uptake, biotransformation, and efflux of tea polyphenol (-)-epigallocatechin-3-gallate in HT-29 human colon adenocarcinoma cells[J]. Cancer Research, 2002, 62(24): 7241-7246.
[16] Awad HM, Boersma MG, Boeren S, et al. Structure-activity study on the quinone/quinone methide chemistry of flavonoids[J]. Chemical Research in Toxicology, 2001, 14(4): 398-408.
[17] Sang S, Buckley B, Ho CT, et al. Autoxidative quinone formation in vitro and metabolite formation in vivo from tea polyphenol (-)-epigallocatechin-3-gallate: studied by real-time mass spectrometry combined with tandem mass ion mapping[J]. Free Radical Biology and Medicine, 2007, 43(3): 362-371.
[18] Yang G-Y, Liao J, Kim K, et al. Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols[J]. Carcinogenesis, 1998, 19(4): 611-616.
[19] Elbling L, Weiss RM, Teufelhofer O, et al. Green tea extract and (-)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities[J]. The FASEB Journal, 2005, 19(7): 807-809.
[20] Halliwell B.Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies?[J]. Archives of Biochemistry and Biophysics, 2008, 476(2): 107-112.
[21] Isbrucker R, Bausch J, Edwards J, et al. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 1: genotoxicity[J]. Food and Chemical Toxicology, 2006, 44(5): 626-635.
[22] Long LH, Halliwell B.Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media[J]. Biochemical and Biophysical Research Communications, 2009, 388(4): 700-704.
[23] Dashwood W-M, Orner GA, Dashwood RH.Inhibition of β-catenin/Tcf activity by white tea, green tea, and epigallocatechin-3-gallate (EGCG): minor contribution of H2O2 at physiologically relevant EGCG concentrations[J]. Biochemical and Biophysical Research Communications, 2002, 296(3): 584-588.
[24] Nakagawa H, Hasumi K, Woo J-T, et al. Generation of hydrogen peroxide primarily contributes to the induction of Fe (II)-dependent apoptosis in Jurkat cells by (-)-epigallocatechin gallate[J]. Carcinogenesis, 2004, 25(9): 1567-1574.
[25] Li GX, Chen YK, Hou Z, et al. Pro-oxidative activities and dose-response relationship of (-)-epigallocatechin-3-gallate in the inhibition of lung cancer cell growth: a comparative study in vivo and in vitro[J]. Carcinogenesis, 2010, 31(5): 902-910.
[26] Hou Z, Xiao H, Lambert J, et al. Green tea polyphenol, (-)-epigallocatechin-3-gallate, induces oxidative stress and DNA damage in cancer cell lines, xenograft tumors, and mouse liver[J]. Proceedings of the American Association for Cancer Research, 2006, 66(1): 1150.
[27] Kim HS, Quon MJ, Kim JA.New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate[J]. Redox Biology, 2014, 2: 187-195.
[28] Azam S, Hadi N, Khan NU, et al. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties[J]. Toxicology in Vitro, 2004, 18(5): 555-561.
[29] Lambert JD, Elias RJ.The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention[J]. Archives of Biochemistry and Biophysics, 2010, 501(1): 65-72.
[30] Tao L, Forester SC, Lambert JD.The role of the mitochondrial oxidative stress in the cytotoxic effects of the green tea catechin, (-)-epigallocatechin-3-gallate, in oral cells[J]. Molecular nutrition & Food Research, 2014, 58(4): 665-676.
[31] Bae YS, Lee JH, Choi SH, et al. Macrophages Generate Reactive Oxygen Species in Response to Minimally Oxidized LDL: TLR4- and Syk-Dependent activation of NADPH Oxidase 2[J]. Circulation Research, 2009, 104(2): 210-218.
[32] Wu C, Hsu M, Hsieh C, et al. Upregulation of heme oxygenase-1 by Epigallocatechin-3-gallate via the phosphatidylinositol 3-kinase/Akt and ERK pathways[J]. Life Sciences, 2006, 78(25): 2889-2897.
[33] Pullikotil P, Chen H, Muniyappa R, et al. Epigallocatechin gallate induces expression of heme oxygenase-1 in endothelial cells via p38 MAPK and Nrf-2 that suppresses proinflammatory actions of TNF-α[J]. The Journal of Nutritional Biochemistry, 2012, 23(9): 1134-1145.
[34] Sakagami H, Arakawa H, Maeda M, et al. Production of hydrogen peroxide and methionine sulfoxide by epigallocatechin gallate and antioxidants[J]. Anticancer Research, 2000, 21(4A): 2633-2641.
[35] Yang G-Y, Liao J, Li C, et al. Effect of black and green tea polyphenols on c-jun phosphorylation and H2O2 production in transformed and non-transformed human bronchial cell lines: possible mechanisms of cell growth inhibition and apoptosis induction[J]. Carcinogenesis, 2000, 21(11): 2035-2039.
[36] Collins QF, Liu HY, Pi J, et al. Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, suppresses hepatic gluconeogenesis through 5′-AMP-activated protein kinase[J]. Journal of Biological Chemistry, 2007, 282(41): 30143-30149.
[37] Qanungo S, Das M, Haldar S, et al. Epigallocatechin-3-gallate induces mitochondrial membrane depolarization and caspase-dependent apoptosis in pancreatic cancer cells[J]. Carcinogenesis, 2005, 26(5): 958-967.
[38] Waltner-Law ME, Wang XL, Law BK, et al. Epigallocatechin gallate, a constituent of green tea, represses hepatic glucose production[J]. Journal of Biological Chemistry, 2002, 277(38): 34933-34940.
文章导航

/