The study was aimed to understand the effects of Pu-erh theabrownin (TB) (Mw>50βkDa) on the metabolism of rat urine by nuclear magnetic resonance (NMR)-based metabolomics. Rats were divided into four groups including rats receiving a basic diet alone (control group), a basic diet and intragastric infusion of Pu-erh TBs (TB infusion group), a high-lipid diet alone (high lipid group) and high-lipid diet and intragastric infusion of Pu-erh TBs (high lipid-TB infusion group) for 14 d. Urine samples were analyzed by biochemical assays, NMR and orthogonal partial least-square-discriminant analysis (OPLS-DA) to identify differential metabolism among these groups. The results showed that considerable differences in metabolite profiles of rat urine were detected between control group and TB infusion group, and between high lipid group and high lipid-TB infusion group. Valine, citric acid, taurine, propionate, α-ketoglutaric acid and β-hydroxybutyrate were identified as metabolic markers of Pu-erh TB. Besides, Pu-erh TB was likely to influence several metabolic pathways in rat, including amino acid metabolism, energy metabolism, tricarboxylic acid cycle, lipid metabolism and oxidative stress.
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