普洱茶茶褐素对大鼠尿液影响的代谢组学研究

doi:10.13305/j.cnki.jts.2016.05.007

摘要/Abstract

摘要： 本研究采用核磁共振（NMR）代谢组学法研究普洱茶茶褐素（TB,分子量>50βkDa）对大鼠尿液代谢的影响。将实验大鼠分为正常对照组、正常+TB组、高脂组和高脂+TB组,以生物核磁共振技术结合正交偏最小二乘-判别分析（OPLS-DA）研究灌胃高剂量普洱茶茶褐素后大鼠尿液内源性代谢物的变化。结果表明,正常+TB组与正常对照组,高脂+TB组与高脂组的尿液代谢谱有明显差异;筛选出缬氨酸、柠檬酸、牛磺酸、丙酸盐、α-酮戊二酸、β-羟基丁酸等6种普洱茶茶褐素标志性代谢物;普洱茶茶褐素对大鼠尿液的影响可能涉及到氨基酸代谢、能量代谢、三羧酸循环、脂类代谢和氧化应激反应。

关键词: 茶褐素, 代谢组学, 核磁共振

Abstract: The study was aimed to understand the effects of Pu-erh theabrownin (TB) (Mw>50βkDa) on the metabolism of rat urine by nuclear magnetic resonance (NMR)-based metabolomics. Rats were divided into four groups including rats receiving a basic diet alone (control group), a basic diet and intragastric infusion of Pu-erh TBs (TB infusion group), a high-lipid diet alone (high lipid group) and high-lipid diet and intragastric infusion of Pu-erh TBs (high lipid-TB infusion group) for 14 d. Urine samples were analyzed by biochemical assays, NMR and orthogonal partial least-square-discriminant analysis (OPLS-DA) to identify differential metabolism among these groups. The results showed that considerable differences in metabolite profiles of rat urine were detected between control group and TB infusion group, and between high lipid group and high lipid-TB infusion group. Valine, citric acid, taurine, propionate, α-ketoglutaric acid and β-hydroxybutyrate were identified as metabolic markers of Pu-erh TB. Besides, Pu-erh TB was likely to influence several metabolic pathways in rat, including amino acid metabolism, energy metabolism, tricarboxylic acid cycle, lipid metabolism and oxidative stress.

Key words: theabrownins, metabolomics, NMR

中图分类号:

刘建, 彭春秀, 盛军, 高斌, 龚加顺. 普洱茶茶褐素对大鼠尿液影响的代谢组学研究[J]. 茶叶科学, 2016, 36(5): 491-499. doi: 10.13305/j.cnki.jts.2016.05.007.

LIU Jian, PENG Chunxiu, SHENG Jun, GAO Bin, GONG Jiashun. Metabolomic Analysis of Rat Urine after Intragastric Infusion of Pu-erh Theabrownins[J]. Journal of Tea Science, 2016, 36(5): 491-499. doi: 10.13305/j.cnki.jts.2016.05.007.

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