六堡茶茶褐素体外降脂功效研究

doi:10.13305/j.cnki.jts.20200612.003

摘要/Abstract

摘要： 考察六堡茶茶褐素体外结合胆酸盐能力、抑制胰脂肪酶活性及胆固醇酯酶活性能力、吸附胆固醇及油脂能力,从而评价其体外降脂活性。研究结果显示,六堡茶茶褐素对花生油的吸附量为0.73 g·g^-1,对胆固醇的吸附量在酸性条件下为122.42 mg·g^-1、中性条件下为13.68 mg·g^-1;在茶褐素结合胆酸盐的试验中,随着茶褐素浓度的增加,与胆酸盐结合能力也呈上升趋势;茶褐素对胰脂肪酶起激活作用,且随着浓度的增加呈持续上升趋势;对胆固醇酯酶活性抑制IC₅₀值为57.2 mg·mL^-1。六堡茶茶褐素降脂机制可能主要通过与胆酸盐结合、吸附胆固醇和脂肪来实现。

关键词: 六堡茶, 茶褐素, 体外降脂, 胆酸盐

Abstract: To evaluate the hypolipidemic effects of theabrownins in Liupao tea, their abilities to bind cholate, inhibit pancreatic lipase and cholesterol esterase, adsorb cholesterol and lipid in vitro were determined. The results show that the adsorption capacity of peanut oil by theabrownins was 0.73 g·g^-1, and the adsorption amount of cholesterol was 122.42 mg·g^-1 under acid condition and 13.68 mg·g^-1 under neutral condition. In the theabrownin-binding cholate test, the binding abilities to the cholate showed an upward trend with the increase of theabrownin concentrations. However, the figures indicate that theabrownins can activate pancreatic lipase and it continues to increase with the increase of concentration. The IC₅₀ value of inhibition on cholesterol esterase activity was 57.2 mg·mL^-1. The hypolipidemic mechanism of theabrownins in Liupao tea might be mainly achieved by binding to cholate and adsorbing cholesterol and lipid.

Key words: Liupao tea, theabrownin, hypolipidemic effects, cholate

中图分类号:

龚受基, 滕翠琴, 梁东姨, 曹惠怡, 蒙彦妃, 张均伟, 谢加仕. 六堡茶茶褐素体外降脂功效研究[J]. 茶叶科学, 2020, 40(4): 536-543. doi: 10.13305/j.cnki.jts.20200612.003.

GONG Shouji, TENG Cuiqin, LIANG Dongyi, CAO Huiyi, MENG Yanfei, ZHANG Junwei, XIE Jiashi. In Vitro Study on Hypolipidemic Effects of Theabrownins in Liupao Tea[J]. Journal of Tea Science, 2020, 40(4): 536-543. doi: 10.13305/j.cnki.jts.20200612.003.

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