六堡茶对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响

doi:10.13305/j.cnki.jts.2014.03.004

茶叶科学 ›› 2014, Vol. 34 ›› Issue (3): 230-238.doi: 10.13305/j.cnki.jts.2014.03.004

六堡茶对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响

滕翠琴^1,2, 刘仲华^1,2,*, 龚受基³, 彭雨轩⁴, 马蕊⁵

1. 国家植物功能成分利用工程技术研究中心,湖南长沙 410128;
2. 湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
3. 桂林医学院药学院,广西桂林 541004;
4. 湖南城市学院黑茶研究所,湖南益阳 413000;
5. 广西职业技术学院,广西南宁 530226

收稿日期:2013-07-12 修回日期:2013-09-16 出版日期:2014-06-15 发布日期:2019-09-03
通讯作者: *larkin-liu@163.com
作者简介:滕翠琴（1986— ）,女,广西南宁人,硕士研究生,主要从事功能成分开发与利用研究。
基金资助:
国家茶叶产业技术体系研究项目（CARS-23-10B）、国家自然科学基金项目（31100502）资助

Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes

TENG Cuiqin^1,2, LIU Zhonghua^1,2,*, GONG Shouji³, PENG Yuxuan⁴, Ma Rui⁵

1. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
2. Key Lab of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
3. College of Pharmacy, Guilin Medical University, Guilin 541004, China;
4. Dark Tea Studies Insitute, Hunan City University, Yiyang 413000, China;
5. Guangxi Vocational & Technical College, Nanning 530226, China

Received:2013-07-12 Revised:2013-09-16 Online:2014-06-15 Published:2019-09-03

摘要/Abstract

摘要： 采用软脂酸诱导3T3-L1脂肪细胞胰岛素抵抗后,给予不同剂量的六堡茶水提物治疗,探讨六堡茶对胰岛素抵抗3T3-L1脂肪细胞的糖脂代谢的影响。结果表明,1 mmol·L^-1的软脂酸对3T3-L1脂肪细胞作用24 h后,葡萄糖摄取量减少,建立胰岛素抵抗模型。六堡茶对模型细胞干预24 h后,与模型组比较,细胞培养基的残留液中葡萄糖和游离脂肪酸的含量降低;对糖脂代谢相关关键酶（己糖激酶、6-磷酸果糖激酶-1、丙酮酸激酶、甘油-3-磷酸酰基转移酶）和葡萄糖转运子基因表达都有上调作用,并下调乙酰辅酶A羧化酶、蛋白酪氨酸磷酸酯酶1B基因表达。低剂量的六堡茶下调肉碱脂酰转移酶-I基因的表达,但是高剂量能显著上调肉碱脂酰转移酶-I基因表达。实验证明了六堡茶能够提高胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取能力,同时促进细胞内糖脂代谢,六堡茶有改善3T3-L1脂肪细胞胰岛素抵抗的作用。

关键词: 六堡茶, 糖脂代谢, 胰岛素抵抗, 3T3-L1脂肪细胞

Abstract: To investigate the effect of Liupao tea on glucose and lipid metabolism in palmitate-induced insulin resistance 3T3-L1 adipocytes. Glucose intake decreased and insulin resistance was induced in 3T3-L1 adipocytes after incubation in 1mmol/L palmitate for 24 hours. Compared with the model group, the concentrations of glucose and NEFA decreased along with the increase of Liupao tea concentrations in medium. The mRNA expression of key enzyme for glycolysis of glucose (Hexokinase, 6-phosphofructokinase-1, and pyruvatekinase) and the Lipid-Metabolism-Related Enzymes (Sn-glycerol-3-phosphate acyltransferase) significantly increased in the Liupao tea groups comparing with model sample. Meanwhile the glucose transporter 4 mRNA expression was also increased and the acetyl-CoA carboxylase, protein tyrosine phosphatase 1B mRNA expression was decreased. Low doses of Liupao tea reduced carnitine palmitoyl transferase I mRNA expression while high doses significantly raised it. Research proved that Liupao tea were able to increase glucose uptake and activate the glucolipid metabolic pathways. Liupao tea showed the effect of improving insulin resistance induced by palmitate in 3T3 -L1 adipocytes.

Key words: Liupao tea, glucolipid metabolism, insulin resistance, 3T3-L1 adipocytes

中图分类号:

TS272.5⁺4

滕翠琴, 刘仲华, 龚受基, 彭雨轩, 马蕊. 六堡茶对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响[J]. 茶叶科学, 2014, 34(3): 230-238. doi: 10.13305/j.cnki.jts.2014.03.004.

TENG Cuiqin, LIU Zhonghua, GONG Shouji, PENG Yuxuan, Ma Rui. Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes[J]. Journal of Tea Science, 2014, 34(3): 230-238. doi: 10.13305/j.cnki.jts.2014.03.004.

参考文献 23

[1]	WU Y, DING L, XIA H, et al. Analysis of the major chemical compositions in Fuzhuan brick-tea and its effect on activities of pancreatic enzymes in vitro[J]. African Journal of Biotechnology, 2010, 9(40): 6748-6754.
[2]	傅冬和, 刘仲华, 黄建安, 等. 茯砖茶加工过程中主要化学成分的变化[J]. 食品科学, 2008, 29(2): 64-67.
[3]	ZHANG L, ZHANG Z-Z, ZHOU Y-B, et al. Chinese dark teas: Postfermentation, chemistry and biological activities[J]. Food Research International, 2013, 53(2): 600-607.
[4]	LU C-H, HWANG L S.Polyphenol contents of Pu-Erh teas and their abilities to inhibit cholesterol biosynthesis in Hep G2 cell line[J]. Food Chemistry, 2008, 111(1): 67-71.
[5]	FU D, RYAN E P, HUANG J, et al. Fermented Camellia sinensis,Fu Zhuan Tea, regulates hyperlipidemia and transcription factors involved in lipid catabolism[J]. Food Research International, 2011, 44(9): 2999-3005.
[6]	龚受基. 六堡茶和茉莉花改善胰岛素抵抗功效及机制研究[D]. 长沙: 湖南农业大学, 2012: 49-79.
[7]	彭静静. 六堡茶的降血脂功能性研究[D]. 南宁: 广西大学, 2012: 28-38.
[8]	SKRZYPSKI M, KACZMAREK P, LE T, et al. Effects of orexin A on proliferation, survival, apoptosis and differentiation of 3T3-L1 preadipocytes into mature adipocytes[J]. FEBS Lett, 2012,586(23):4157-4164.
[9]	CHANGSUK K, JUNGAE K, SEKWON K.Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes[J]. Food Chem Toxicol, 2009, 47(10): 2401-2406.
[10]	GAO C-L, ZHU C, ZHAO Y-P, et al. Mitochondrial dysfunction is induced by high levels of glucose and free fatty acids in 3T3-L1 adipocytes[J]. Mol Cell Endocrinol, 2010, 320(1/2): 25-33.
[11]	KERSHAW E E, FLIER J S.Adipose tissue as an endocrine organ[J]. J Clin Endocrinol Metab, 2004, 89(6): 2548-2556.
[12]	CAPURSO C, CAPURSO A.From excess adiposity to insulin resistance: The role of free fatty acids[J]. Vascul Pharmacol, 2012, 57(2/4): 91-97.
[13]	SUKHATME V P, CHAN B.Glycolytic cancer cells lacking 6-phosphogluconate dehydrogenase metabolize glucose to induce senescence[J]. FEBS Lett, 2012, 586(16): 2389-2395.
[14]	PAPAGIANNI M, AVRAMIDIS N.Lactococcus lactis as a cell factory: A twofold increase in phosphofructokinase activity results in a proportional increase in specific rates of glucose uptake and lactate formation[J]. Enzyme Microb Technol, 2011, 49(2): 197-202.
[15]	GUO X, LI H, XU H, et al. Glycolysis in the control of blood glucose homeostasis[J]. Acta Pharmaceutica Sinica B, 2012, 2(4): 358-367.
[16]	CARPENTIER A C.Postprandial fatty acid metabolism in the development of lipotoxicity and type 2 diabetes[J]. Diabetes Metab, 2008, 34(2): 97-107.
[17]	KOK B, BRINDLEY D N.Myocardial Fatty Acid metabolism and lipotoxicity in the setting of insulin resistance[J]. Heart Fail Clin, 2012, 8(4): 643-661.
[18]	TUEI V C, HA J-S, HA C-E.Effects of human serum albumin complexed with free fatty acids on cell viability and insulin secretion in the hamster pancreatic -cell line HIT-T15[J]. Life Sci, 2011, 88(17): 810-818.
[19]	FOSTER D W.The role of the carnitine system in human metabolism[J]. Ann N Y Acad Sci, 2004, 1033(1): 1-16.
[20]	CHIU T T, JENSEN T E, SYLOW L, et al. Rac1 signalling towards GLUT4/glucose uptake in skeletal muscle[J]. Cell Signal, 2011, 23(10): 1546-1554.
[21]	BERENGUER M, MARCHAND-BRUSTEL Y L, GOVERS R. GLUT4 molecules are recruited at random for insertion within the plasma membrane upon insulin stimulation[J]. FEBS Lett, 2010, 584(3): 537-542.
[22]	WANG N, ZHANG D, MAO X, et al. Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes[J]. Mol Cell Endocrinol, 2009, 307(1): 89-98.
[23]	张栩颜, 黄宇声, 刘冠萍, 等. 六堡茶对高脂血症小鼠血脂及脂质过氧化的影响[J]. 医学理论与实践, 2013, 26(5): 563-564.

六堡茶对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响

Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 23

相关文章 14

Metrics

本文评价

推荐阅读 10

[1]	孙颖, 陈鑫, 杨华, 应剑, 邵丹青, 吕晓华, 肖杰, 陈志雄, 李颂, 覃俊杰, 郑斌, 高建设. 饮用金花香橼茶3个月对小样本高脂血症人群糖脂代谢的改善效果研究[J]. 茶叶科学, 2022, 42(4): 561-576.
[2]	黄秋萍, 谢晨阳, 李新宇, 金斌, 曾榛, 钱波, 戴玲, 宋家乐. L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状[J]. 茶叶科学, 2021, 41(6): 831-842.
[3]	王绍梅, 李晓君, 宋文明, 潘联云. 普洱茶中没食子酸及其改善饮食诱导的糖脂代谢紊乱研究进展[J]. 茶叶科学, 2020, 40(4): 431-440.
[4]	龚受基, 滕翠琴, 梁东姨, 曹惠怡, 蒙彦妃, 张均伟, 谢加仕. 六堡茶茶褐素体外降脂功效研究[J]. 茶叶科学, 2020, 40(4): 536-543.
[5]	马婉君, 马士成, 刘春梅, 龙志荣, 唐保军, 林智, 吕海鹏. 六堡茶的化学成分及生物活性研究进展[J]. 茶叶科学, 2020, 40(3): 289-304.
[6]	叶颖, 韦保耀, 滕建文, 夏宁, 黄丽, 曾司斌. 六堡茶对高脂饮食大鼠肠道短链脂肪酸含量的影响[J]. 茶叶科学, 2019, 39(2): 211-219.
[7]	吴文亮, 刘仲华, 林勇, 黄建安, 左高隆, 滕翠琴, 龙志荣, 邱瑞瑾, 曹中环. 陈年六堡茶对高脂血症小鼠的调脂护肝作用研究[J]. 茶叶科学, 2018, 38(4): 430-438.
[8]	吴文亮, 林勇, 刘仲华, 黄建安, 龙志荣, 滕翠琴, 马士成, 邱瑞瑾, 曹中环. 六堡茶急性和亚急性毒性安全性评价研究[J]. 茶叶科学, 2017, 37(2): 173-181.
[9]	应乐, 潘越, 王岳飞, 徐平. 六堡茶多糖理化性质、体外抗氧化及其对人脐静脉血管内皮细胞的保护作用[J]. 茶叶科学, 2017, 37(1): 25-37.
[10]	吴平. 茶叶分类进展研究——兼论六堡茶的归属[J]. 茶叶科学, 2014, 34(4): 408-416.
[11]	夏家杰, 薛蒙, 金梦, 吴珏莅, 刘含登. 茶多酚改善胰岛素抵抗的初步研究[J]. 茶叶科学, 2014, 34(2): 144-148.
[12]	毛彦, 黄丽, 韦保耀, 夏宁, 滕建文. 广西六堡茶“金花”菌的分离与分子鉴定[J]. 茶叶科学, 2013, 33(6): 556-561.
[13]	王文祥, 苏畅, 廖惠珍. 茶色素对胰岛素抵抗大鼠胰岛素受体的影响[J]. 茶叶科学, 2008, 28(6): 455-458.
[14]	胡月龄, 胡建程, 钱泽澍. 压制茶中霉菌的研究[J]. 茶叶科学, 1984, 4(01): 53-58.