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茶叶科学 ›› 2018, Vol. 38 ›› Issue (4): 430-438.doi: 10.13305/j.cnki.jts.2018.04.012

• • 上一篇    

陈年六堡茶对高脂血症小鼠的调脂护肝作用研究

吴文亮1,2,3,4, 刘仲华1,2,3,*, 林勇1,2,3,*, 黄建安1,2,3, 左高隆1,2, 滕翠琴5, 龙志荣6, 邱瑞瑾5, 曹中环5   

  1. 1. 湖南农业大学茶学教育部重点实验室,湖南 长沙 410128;
    2. 国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;
    3. 植物功能成分利用协同创新中心,湖南 长沙 410128;
    4. 湖南省农业科学院茶叶研究所,湖南 长沙 410125;
    5. 梧州市六堡茶研究院,广西 梧州 543003;
    6. 梧州市茶产业发展办公室,广西 梧州543003
  • 收稿日期:2017-12-12 修回日期:2018-01-30 出版日期:2018-08-15 发布日期:2019-10-15
  • 通讯作者: *larkin-liu@163.com
  • 作者简介:吴文亮,男,在读博士研究生,主要从事茶叶加工及茶叶功能成分化学研究。
  • 基金资助:
    梧州市科学研究与技术开发计划项目(201501028),2015年梧州市六堡茶产业化项目,国家茶叶产业技术体系岗位科学家项目(CARS-23)、湖南省自然科学基金项目(2017JJ3106)

Alleviative Effects of Aged Liupao Tea on Lipid Metabolism and Liver Injury in Hyperlipidemic Mice

WU Wenliang1,2,3,4, LIU Zhonghua1,2,3,*, LIN Yong1,2,3,*, HUANG Jian′an1,2,3, ZUO Gaolong1,2, TENG Cuiqin5, LONG Zhirong6, QIU Ruijin5, CAO Zhonghuan5   

  1. 1. Key Lab of Tea Science of Education Ministry, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China;
    4. Tea of Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;
    5. Wuzhou Academy of Liupao Tea, Wuzhou 543003, China;
    6. Wuzhou of Office Tea Industry Development, Wuzhou 543003, China
  • Received:2017-12-12 Revised:2018-01-30 Online:2018-08-15 Published:2019-10-15

摘要: 为了探讨陈年六堡茶的调脂保肝功效,比较分析了不同年代六堡茶的主要化学物质的差异,再以高脂高糖饮食建立高脂血症小鼠模型,并用选定的陈年六堡茶水提物灌胃小鼠,观察小鼠相关生理生化指标及组织形态的变化。结果表明,陈化15年茶样被选为陈年六堡茶的代表,用于后续试验。与模型组相比,陈年六堡茶尤其中高剂量条件下能有效减轻高脂血症小鼠体重、肝重和降低肝系数,显著提高高密度脂蛋白-胆固醇(HDL-C)含量,降低血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白-胆固醇(LDL-C)含量,降低谷丙转氨酶(ALT)和谷草转氨酶(AST)活力,改善大鼠肝脏氧化应激状态,具有统计学意义(P<0.05或P<0.01)。肝脏和脂肪组织病理切片显示,陈年六堡茶能明显减少肝细胞中脂滴的形成和抑制脂肪细胞膨大,呈剂量依赖关系。综上表明,陈年六堡茶在正常剂量条件下能有效改善因高脂高糖饮食所致的小鼠脂质代谢紊乱和肝脏损伤。

关键词: 陈年六堡茶, 高脂血症, 脂质代谢, 肝损伤, 改善作用

Abstract: To investigate the alleviative effects of aged Liupao tea on lipid metabolism and liver injury in hyperlipidemic mice, the changes of main chemical substances in Liupao tea of different storage ages were analyzed. The hyperlipidemia model mice were established by feeding a high fat and sugar diet, and Liupao tea groups were given daily oral administration of water extract of selected aged Liupao tea. The changes of related physiological and biochemical indexes and tissue morphology in mice were observed. The results showed that Liupao tea samples stored for 15 years were selected as the representative of the aged Liupao tea, which was used in subsequent experiments. Compared with the hyperlipidemia model group, aged Liupao tea especially medium or high dose groups could significantly decrease the contents of TC, TG, and LDL-C levels, increase the content of HDL-C, suppress the activities of ALT and AST in the serum, and moderate liver oxidative stress,. Pathological sections of liver and adipose tissue showed that aged Liupao tea could reduce the formation of lipid droplets in liver cells and inhibit the enlargement of fat cells in a dose dependent manner. In conclusion, aged Liupao tea of normal dose could effectively improve lipid metabolism disorder and liver injury in mice induced by high fat and sugar diet.

Key words: aged Liupao tea, hyperlipidemia, lipid metabolism, liver injury, alleviative effect

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