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茶叶科学 ›› 2008, Vol. 28 ›› Issue (1): 67-71.doi: 10.13305/j.cnki.jts.2008.01.012

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茶多糖对四氯化碳致小鼠肝损伤的保护作用

方伟1, 陈彦1,*, 孙玉军1,2   

  1. 1. 安徽大学生命科学学院 安徽省中药研究与开发重点实验室,安徽 合肥 230039;
    2. 安徽科技学院生命科学学院,安徽 凤阳 233100
  • 收稿日期:2007-06-06 修回日期:2007-10-11 出版日期:2008-02-15 发布日期:2019-09-16
  • 通讯作者: *chenyan600@163.com
  • 作者简介:方伟(1982— ),男,安徽合肥人,硕士研究生,主要从事植物多糖分离纯化及活性研究
  • 基金资助:
    农业部茶叶生物化学与生物技术重点实验室开放基金,安徽省自然基金(070413143)

Protective Effect of Tea Polysaccharides on Carbon Tetrachloride Induced Liver Injury in Mice

FANG Wei1, CHEN Yan1,*, SUN Yu-jun1,2   

  1. 1. School of Life Science, Anhui University, The key lab of Chinese traditional medicine research and development of Anhui Province, Hefei 230039, China;
    2. School of Life Science, Anhui Science and Technology University, Fengyang 233100, China
  • Received:2007-06-06 Revised:2007-10-11 Online:2008-02-15 Published:2019-09-16

摘要: 从六安炒青水提物中分离纯化得到茶多糖TPS-II,对其纯度和分子量进行检测,通过测定小鼠血清超氧化物歧化酶(SOD)活性、天冬氨酸转氨酶(AST)活性、丙二醛(MDA)含量及肝脏指数,研究其对四氯化碳(CCl4)引起的急性肝损伤的保护作用。结果表明TPS-II相对分子量约为1.01×105,总糖含量为85.30%,蛋白含量为2.80%,并对CCl4引起的小鼠急性肝损伤有明显的保护作用。

关键词: 茶多糖, 分离纯化, 肝损伤, 保护作用

Abstract: A polysaccharide named TPS-II was isolated from the water extraction of Liu’an Roasted Green Tea and purified by chromatography. The homogeneity of TPS-II was identified, its protective effect on carbon tetrachloride induced liver injury in mice was investigated. Results indicated that the molecular weight of TPS-II is about 1.01×105, whose total content of saccharide and protein were 85.30% and 2.80% respectively, and its protective effect against CCl4 induced acute liver injury is significant.

Key words: tea polysaccharide, isolation and purification, liver injury, protective effect

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