茶树根系Actin基因克隆及表达分析

李远华; 陆建良; 范方媛; 石玉涛

doi:10.13305/j.cnki.jts.2015.04.005

茶叶科学 >

2015 , Vol. 35 >Issue 4: 336 - 346

DOI: https://doi.org/10.13305/j.cnki.jts.2015.04.005

茶树根系Actin基因克隆及表达分析

李远华 ,
陆建良 ,
范方媛 ,
石玉涛

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1. 武夷学院茶与食品学院/福建省武夷茶资源创新利用重点实验室/福建省高校茶叶工程研究中心/中国乌龙茶产业协同创新中心（培育）,福建武夷山 354300;
2. 浙江大学茶叶研究所,浙江杭州 310058

李远华,男,教授,博士,主要从事茶学及生物技术研究,E-mail: yhli@wuyiu.edu.cn。

收稿日期: 2015-03-11

修回日期: 2015-04-17

网络出版日期: 2019-08-26

基金资助

国家自然科学基金面上项目（31070613）、福建省科技厅重点项目（2012N0025）、国家级大学生创新创业训练计划项目（201310397001）

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Gene Cloning and Expression Analysis of Actin in Tea Plant Root

LI Yuanhua ,
LU Jianliang ,
FAN Fangyuan ,
SHI Yutao

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1. College of Tea and Food Science, Wuyi University, Wuyishan 354300, China;
2. Zhejiang University Tea Research Institute, Hangzhou 310058, China

Received date: 2015-03-11

Revised date: 2015-04-17

Online published: 2019-08-26

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摘要

采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在下调表达序列中可能包含了10种未知功能的基因;在上调表达序列中可能包含了5种可能的基因。采用RACE技术获得了Actin基因全长序列,Actin基因长1β606βbp (GenBank, 登录号KJ946252),具有1β131βbp开放阅读框（1^st~1β131^st）,编码377个氨基酸。分子生物信息学分析表明,Actin蛋白分子量约30.69βkD,等电点为5.27,定位于细胞核等亚细胞区位。研究还显示,Actin在不同品种中表达无显著差异,对非生物性胁迫响应也较弱。

关键词： 茶树; Actin; 基因克隆; 表达

本文引用格式

李远华 , 陆建良 , 范方媛 , 石玉涛 . 茶树根系Actin基因克隆及表达分析[J]. 茶叶科学, 2015 , 35(4) : 336 -346 . DOI: 10.13305/j.cnki.jts.2015.04.005

Abstract

By using SSH, we analyzed differences in gene expression of root from Fuding white tea infected by VA mycorrhiza and obtained diversity sequences. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possible contained 5 known genes. The Actin genic full-length sequence was obtained by using RACE. The length of Actin gene was 1β606βbp (GenBank Accession No. KJ946252), with 1β131βbp ORF (1^st-1β131^st), the sequence encoded 377 amino acid. Bioinformatics indicated that the Actin protein’s molecular weight was about 30.69 kD, IEP was 5.27, located in subcellular fraction area like cell nucleus. The study also showed Actin expressed no obvious difference in different cultivars and it responded weak to non-biological stress.

Key words： tea plant; actin; gene clone; expression

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