茶树根系Actin基因克隆及表达分析

doi:10.13305/j.cnki.jts.2015.04.005

摘要/Abstract

摘要： 采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在下调表达序列中可能包含了10种未知功能的基因;在上调表达序列中可能包含了5种可能的基因。采用RACE技术获得了Actin基因全长序列,Actin基因长1β606βbp (GenBank, 登录号KJ946252),具有1β131βbp开放阅读框（1^st~1β131^st）,编码377个氨基酸。分子生物信息学分析表明,Actin蛋白分子量约30.69βkD,等电点为5.27,定位于细胞核等亚细胞区位。研究还显示,Actin在不同品种中表达无显著差异,对非生物性胁迫响应也较弱。

关键词: 茶树, Actin, 基因克隆, 表达

Abstract: By using SSH, we analyzed differences in gene expression of root from Fuding white tea infected by VA mycorrhiza and obtained diversity sequences. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possible contained 5 known genes. The Actin genic full-length sequence was obtained by using RACE. The length of Actin gene was 1β606βbp (GenBank Accession No. KJ946252), with 1β131βbp ORF (1^st-1β131^st), the sequence encoded 377 amino acid. Bioinformatics indicated that the Actin protein’s molecular weight was about 30.69 kD, IEP was 5.27, located in subcellular fraction area like cell nucleus. The study also showed Actin expressed no obvious difference in different cultivars and it responded weak to non-biological stress.

Key words: tea plant, actin, gene clone, expression

中图分类号:

S571.1
Q52

李远华, 陆建良, 范方媛, 石玉涛. 茶树根系Actin基因克隆及表达分析[J]. 茶叶科学, 2015, 35(4): 336-346. doi: 10.13305/j.cnki.jts.2015.04.005.

LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of Actin in Tea Plant Root[J]. Journal of Tea Science, 2015, 35(4): 336-346. doi: 10.13305/j.cnki.jts.2015.04.005.

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