钙离子信号通路在植物抗寒响应中起着重要作用。为了解钙离子信号通路与茶树抗寒性之间的关系,采用人工气候室模拟低温胁迫和外源施用钙调素（CaM）抑制剂W-7[N-(6-Aminohexyl)-5-chloro-1 -naphthalenesulfonamide]、钙信号通道抑制剂LaCl3及CaCl2溶液的方法,测定了低温胁迫下茶树叶片各种与抗寒相关的生理指标的变化情况。检测结果表明,钙离子信号抑制剂W-7、LaCl3处理均能提高茶树叶片相对电导率,提高茶树叶片中丙二醛（MDA）、超氧阴离子自由基（O2·-）、脯氨酸的含量,抑制超氧化物歧化酶（SOD）的活性;而CaCl2溶液处理茶树叶片相对电导率及叶片中MDA、超氧阴离子自由基和脯氨酸的含量降低,SOD活性提高。表明钙离子信号系统在茶树抗寒过程中发挥了重要作用。
The calcium signal transduction pathway plays a pivotal role in the response to the stress of low temperatures. In order to verify the relationship between calcium signal transduction pathway and cold resistance of tea plant, the changes of some cold resistance related index were measured under artificial low temperature stress in climatron accompanied with using exogenous CaCl2, CaM inhibitor W-7 [N-(6-Aminohexyl)-5-chloro-1-naphthalene-sulfonamide] and calcium signal channel inhibitor LaCl3, respectively. Calcium signaling transduction pathway inhibitors W-7 and LaCl3 improved electrolyte leakage, enhanced contents of malondialdehyde (MDA), superoxide anion radical (O2·-) and proline, reduced the activities of superoxide dismutase (SOD) compared with control treatment, however, CaCl2 treatment showed opposite effects. It showed that calcium signaling system play an important role in the response to low temperature stress in tea plant.
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