外源Ca2+及钙离子信号抑制剂对茶树抗寒性的影响

黄玉婷; 钱文俊; 王博; 曹红利; 王璐; 郝心愿; 王新超; 杨亚军

doi:10.13305/j.cnki.jts.2015.06.002

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茶叶科学 ›› 2015, Vol. 35 ›› Issue (6) : 520-526. DOI: 10.13305/j.cnki.jts.2015.06.002

外源Ca²⁺及钙离子信号抑制剂对茶树抗寒性的影响

黄玉婷¹, 钱文俊^1,2, 王博¹, 曹红利¹, 王璐¹, 郝心愿¹, 王新超^1,*, 杨亚军^1,*

作者信息 +

Effects of Exogenous Calcium and Inhibitors of Calcium Signaling Transduction Pathway on Cold Resistance of Tea Plant

HUANG Yuting¹, QIAN Wenjun^1,2, WANG Bo¹, CAO Hongli¹, WANG Lu¹, HAO Xinyuan¹, WANG Xinchao^1,*, YANG Yajun^1,*

Author information +

文章历史 +

摘要

钙离子信号通路在植物抗寒响应中起着重要作用。为了解钙离子信号通路与茶树抗寒性之间的关系,采用人工气候室模拟低温胁迫和外源施用钙调素（CaM）抑制剂W-7[N-(6-Aminohexyl)-5-chloro-1 -naphthalenesulfonamide]、钙信号通道抑制剂LaCl₃及CaCl₂溶液的方法,测定了低温胁迫下茶树叶片各种与抗寒相关的生理指标的变化情况。检测结果表明,钙离子信号抑制剂W-7、LaCl₃处理均能提高茶树叶片相对电导率,提高茶树叶片中丙二醛（MDA）、超氧阴离子自由基（O₂·^-）、脯氨酸的含量,抑制超氧化物歧化酶（SOD）的活性;而CaCl₂溶液处理茶树叶片相对电导率及叶片中MDA、超氧阴离子自由基和脯氨酸的含量降低,SOD活性提高。表明钙离子信号系统在茶树抗寒过程中发挥了重要作用。

Abstract

The calcium signal transduction pathway plays a pivotal role in the response to the stress of low temperatures. In order to verify the relationship between calcium signal transduction pathway and cold resistance of tea plant, the changes of some cold resistance related index were measured under artificial low temperature stress in climatron accompanied with using exogenous CaCl₂, CaM inhibitor W-7 [N-(6-Aminohexyl)-5-chloro-1-naphthalene-sulfonamide] and calcium signal channel inhibitor LaCl₃, respectively. Calcium signaling transduction pathway inhibitors W-7 and LaCl₃ improved electrolyte leakage, enhanced contents of malondialdehyde (MDA), superoxide anion radical (O₂·^-) and proline, reduced the activities of superoxide dismutase (SOD) compared with control treatment, however, CaCl₂ treatment showed opposite effects. It showed that calcium signaling system play an important role in the response to low temperature stress in tea plant.

导出引用

黄玉婷, 钱文俊, 王博, 曹红利, 王璐, 郝心愿, 王新超, 杨亚军. 外源Ca²⁺及钙离子信号抑制剂对茶树抗寒性的影响[J]. 茶叶科学. 2015, 35(6): 520-526 https://doi.org/10.13305/j.cnki.jts.2015.06.002

HUANG Yuting, QIAN Wenjun, WANG Bo, CAO Hongli, WANG Lu, HAO Xinyuan, WANG Xinchao, YANG Yajun. Effects of Exogenous Calcium and Inhibitors of Calcium Signaling Transduction Pathway on Cold Resistance of Tea Plant[J]. Journal of Tea Science. 2015, 35(6): 520-526 https://doi.org/10.13305/j.cnki.jts.2015.06.002

中图分类号： S571.1 O614.23+1

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