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Journal of Tea Science ›› 2006, Vol. 26 ›› Issue (3): 195-198.doi: 10.13305/j.cnki.jts.2006.03.008

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Determination of Soluble Monosaccharide in Zlex Lalifotia Thumb by GC

JIANG Bo, GUAN Zi-fng, BAI Song-tao, FAN Sheng-di   

  1. College of Life Science, Dalian Nationalities University, Dalian 116600, China
  • Received:2005-11-22 Revised:2006-07-11 Online:2006-09-25 Published:2019-09-11

Abstract: Monosaccharide in Kuding tea(Zelex lalifotia thumb) was obtained by dipping Kuding Tea in water bath with constant temperature, and then drying till the extract solution dryness. The dried monosaccharide was derived into trimethylsilyl derivative with hexamethyedisilane(HMDS) and trimethylchlorosilane(TMCS), and dimethyl sulfoxide(DMSO) used as solvent. The monosaccharide was determined by GC with SE-30 capillary column, and myoinositol used as internal standard. The dissolving ability of dimethylformamide and pyridine were compared and the derivative effect of N, O-bitrimethylsilylacetamide (BSA) was determined. The relative standard deviations were below 8.0%. The contents of monosaccharides in Kuding tea were as follows: glucose(0.355%), fructose(0.440%), galactose(4.000%), ribose(0.035%), xylose(0.150%), arabinose(0.006%).

Key words: Kuding Tea, Soluble Monosaccharide, Trimethylsilyl Derivatization, Gas Chromatography, Internal Standard Method

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