The strain TS1 had been isolated from the soil of tea garden, through the determination performance of the bacteria plate experiment, it can produce the antagonistic performance to the ice nucleation active bacteria which existed on tea plant. According to morphological characteristics and 16βS rDNA sequence analysis, the strain TS1 were preliminary identified as the genus Bacillus subtilis. This paper makes it clear of the species and genus about strain TS1 and provides a good foundation for the biocontrol of tea plant frost injury.
HUANG Xiao-qin
,
ZHANG Li-xia
,
LIU Hui-xiang
,
HAN Xiao-yang
,
CHEN Zong-mao
,
LI Duo-chuan
. Screeining and Identification of the Antagonistic Bacterial Strain Against Ice Nucleation Active Bacteria on Tea Plant[J]. Journal of Tea Science, 2013
, 33(6)
: 570
-575
.
DOI: 10.13305/j.cnki.jts.2013.06.006
[1] Takahiro Makino.Ice-nucleation Activity of Bacteria Isolated from Gemmisphere of Tea Trees[J]. Ann Phytopath Soc Japan, 1983, 49: 32-37.
[2] 黄晓琴, 束怀瑞, 刘会香, 等. 山东省茶树冰核细菌的鉴定[J]. 茶叶科学, 2010, 30(3): 191-194.
[3] 黄晓琴. 山东茶树冰核细菌的分离、鉴定及其与霜冻害关系研究[D]. 泰安: 山东农业大学, 2009: 47-54
[4] 李祥云. 杭州市春茶冻害的调查分析和对策措施[J]. 浙江气象, 2004, 25(4): 18-20.
[5] Lindow SE, Amy DC, Barchet WR, et al. Control of frost damage to plants in the field with bacteria ice nucleation inhibitors[J]. Phytopathology, 1978, 12: 138.
[6] 孙福在, 赵廷昌, 牟丰盛, 等. 生防菌和药剂除冰核细菌防御玉米霜冻研究[J]. 自然灾害学报, 2003, 12(4): 115-119.
[7] 孟庆瑞, 李彦慧, 李帅英, 等. 防除杏树(Prunus armeniaca L.)冰核细菌药剂筛选及花期防霜效果[J]. 生态学报, 2007, 27(10): 4191-4196.
[8] 谭湘芳. 四种鞭毛染色方法的比较[J]. 美国中华临床医学杂志, 2003, 5(1): 73-75.
[9] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 63-65.
[10] R E布坎南, N E 吉本斯. 伯杰细菌鉴定手册[M]. 8版. 北京: 科学出版社, 1984: 732-735.
[11] 骆耀平. 茶树栽培学[M]. 4版. 北京: 中国农业出版社, 2010: 288-292.
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