：A method was established to isolate EGCG3"Me and EGCG 4"Me by using column chromatography and preparative high performance liquid chromatography. Crude extracts was obtained from tea leaves by using ethyl acetate and water as solvent .Then the concentracted crude extract was separated on a HP-20 column. The Fr-1 fraction was obtained from the eluate of 80% ethanol and subjected to a preparative high performance liquid chromatography for isolation of target components by using water-acetonitrile as mobile phase. Two compounds were identified with ultraviolet absorbance(UV), nuclear magnetic resonance(NMR), mass spectrometry(MS). They were EGCG3"Me and EGCG4"Me.The purity of them was more than 98%.
LI Yin-hua
,
ZHANG Sheng
,
HUANG Jian-an
,
LIU Zhong-hua
. Isolation and Identification of EGCG3″Me and EGCG4″Me from Tea[J]. Journal of Tea Science, 2012
, 32(4)
: 313
-318
.
DOI: 10.13305/j.cnki.jts.2012.04.005
[1] 宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003: 7.
[2] Sano M, Suzuki M, Miyase T, et al. Novel anti-allergic catechin derivatives isolated from oolong tea[J]. Journal of Agricultural and Food Chemistry, 1999, 47(5): 1906-1910.
[3] Fujimura Y, Tachibana H, Maeda-Yamamoto M, et al. Antiallergic Tea Catechin,(-)-Epigallocatechin-3-O-(3-O- methyl)-gallate, Suppresses FcεRI Expression in Human Basophilic KU812 Cells[J]. Journal of Agricultural and Food Chemistry, 2002, 50: 5729-5734.
[4] Fujimura Y, Umeda D, Yano S, et al. The 67kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG[J]. Biochemical and Biophysical Research Communications, 2007, 364: 79-85.
[5] Suzuki M, Yoshino K, Maeda-Yamamoto M, et al. Inhibitory effects of tea catechins and O-Methylated derivatives of (-)-epigallocatechin-3-O-gallate on mouse type IV allergy[J]. Journal of Agricultural and Food Chemistry, 2000, 48(11): 5649-5653.
[6] Maeda-Yamamoto M, Ema K, Monobe M, et al. The efficacy of early treatment of seasonal allergic rhinitis with Benifuuki green tea containing O-methylated catechin before pollen exposure:an open randomized[J]. Allergology International, 2009, 58(3): 437-444.
[7] 周蓓, 王琳, 李伟, 等. 茶叶中甲基化儿茶素的分离、纯化和高效液相色谱法分析[J]. 分析化学, 2008, 36(4): 494-498.
[8] 罗正飞, 金孝芳, 龚正礼, 等. EGCG3"Me提取与分离工艺研究[J]. 茶叶科学, 2011, 31(3): 218-224.
[9] 罗正飞, 金孝芳, 龚正礼, 等. 高速逆流色谱分离纯化EGCG3"Me的研究[J]. 分析测试学报, 2011, 30(7): 813-816.
[10] 孙业良, 吕海鹏, 林智, 等. 茶叶中EGCG3″Me的分析方法研究[J]. 茶叶科学, 2009, 29(5): 379-384.
[11] Yanase E, Matsumoto E, Shinoda Y, et al. Synthesis of Methyl Derivatives of Epigallocatechin Gallate (EGCg) and Their Stabilities[J]. ITE Letters on Batteries, New Technologies & Medicine (with News), 2005, 6(1): 34-37.
[12] Sheng BiaoWan, Q Ping Dou, Tak Hang Chan. Regiospecific and enantioselective synthesis of methylated metabolites of tea catechins[J]. Tetrahedron, 2006, 62(25): 5897-5904.
浙公网安备 33019902000101号 
