By clustering the 12757 ESTs of tea downloaded from NCBI, a unigene database of tea was successfully built. It was found that the redundancy rate for ESTs from tea was approximately 68.2%. An EST-SNP exploiting system for tea was established preliminarily. The SNP distribution was identified. The occurrence frequency for coding region SNP in tea was appropriately 0.58%. It meant that there was average one SNP in every 200bp of tea ESTs. Furthermore, the hybrid rate for tea genome was deduced to be 0.38%, averagely one hybrid DNA site per 300bp. Totally 818 candidated SNP were exploited from 237 multigene clusters. Then 25 pairs of SNP primers were designed and 75% of these sites were validated to be polymorphism by DNA sequencing.
WANG Li-yuan
,
ZHANG Cheng-cai
,
CHENG Hao
,
WEI Kang
. Characterization of EST-derived SNPs and Development of SNP-markers in Tea (Camellia sinensis)[J]. Journal of Tea Science, 2012
, 32(4)
: 369
-376
.
DOI: 10.13305/j.cnki.jts.2012.04.014
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