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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (4): 369-376.doi: 10.13305/j.cnki.jts.2012.04.014

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Characterization of EST-derived SNPs and Development of SNP-markers in Tea (Camellia sinensis)

WANG Li-yuan, ZHANG Cheng-cai, CHENG Hao*, WEI Kang   

  1. Tea Research Institute, Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China
  • Received:2011-11-02 Revised:2012-03-20 Online:2012-08-15 Published:2019-09-05

Abstract: By clustering the 12757 ESTs of tea downloaded from NCBI, a unigene database of tea was successfully built. It was found that the redundancy rate for ESTs from tea was approximately 68.2%. An EST-SNP exploiting system for tea was established preliminarily. The SNP distribution was identified. The occurrence frequency for coding region SNP in tea was appropriately 0.58%. It meant that there was average one SNP in every 200bp of tea ESTs. Furthermore, the hybrid rate for tea genome was deduced to be 0.38%, averagely one hybrid DNA site per 300bp. Totally 818 candidated SNP were exploited from 237 multigene clusters. Then 25 pairs of SNP primers were designed and 75% of these sites were validated to be polymorphism by DNA sequencing.

Key words: tea plant, Camellia sinensis, EST, SNP

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