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Please wait a minute... For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails Select Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting HUANG Fu-ping, LIANG Yue-rong, LU Jian-liang, CHEN Rong-bing, Mamati G. E., SUN Qing-lei Journal of Tea Science    2004, 24 (3): 183-189.   DOI: 10.13305/j.cnki.jts.2004.03.007 Abstract （330）      PDF（pc） （385KB）（202）       Knowledge map    Save Genetic diversity in 45 Oolong germplasms collected from three provinces, Fujian, Guangdong and Taiwan was evaluated by AFLP fingerprinting based on silver staining technique using 5 primer combinations. The 5 primer combinations generated 208 polymorphic bands. Largest genetic distance between germplasm was 0.481, and the smallest was 0.124. Estimated genetic diversity within the 45 germplasm was up to 0.311. Genetic diversity within Wuyi Mountain population was highest, while genetic diversity within Anxi population was higher than Guangdong and Taiwan populations and lowest within Taiwan population. A dendrogram generated by NT-SYS software indicated that the 45 Oolong tea germplasms could be divided as Wuyi type and Chaoan type. Genetic similarity determined by Popgene 32 software was highest between Anxi and Wuyi populations which was up to 0.9505, and lowest between Taiwan and Chaoan populations which was up to 0.77. It could be inferred that Fujian Oolong tea germplasms originated from Wuyi Mountains. Reference | Related Articles | Metrics Cited: Baidu(102) Select Evaluation on the Function and Toxicity of Extraction of Characteristic Components in Yunnan pu-erh Tea GONG Jia-shun, CHEN Wen-ping, ZHOU Hong-jie, DONG Zhao-jun, ZHANG Yi-fang Journal of Tea Science    2007, 27 (3): 201-210.   DOI: 10.13305/j.cnki.jts.2007.03.011 Abstract （419）      PDF（pc） （2116KB）（285）       Knowledge map    Save ：The anti-fatigue, reduced cholesterol function, acute toxicity and the mutagenicity of extraction of characteristic components in Yunnan pu-erh tea were studied using mouse as experimental animals. It had significant function of anti-fatigue and reduced cholesterol for mouse, its effects was better than that of water extracts of pu-erh tea. The oral acute toxicity in mouse,Ames test,micronucleus test in bone narrow and chromosome abnormality test of characteristic components extraction in Yunnan pu-erh tea were also carried out．The oral LD50 value of this extraction in mouse was more than 10.0βg/kg．The number of relevant strain colonies had no remarkable increase in the Ames test with and without S9 mixture, and the relationship of dose-response was also not found. The micronucleus rates and chromosome abnormality rates at all range of doses had no significant difference compared with the control group. At the dose of 500~5β000βμg/ml, the characteristic components extraction could not cause the increase of mutagenic rate of CHL (Chinese Hamster Lung Fibroblast, CHL) cells. It suggested that the extraction of characteristic components in Yunnan pu-erh tea has no mutagenicity for mouse． Reference | Related Articles | Metrics Cited: Baidu(71) Select Construction of AFLP Molecular Markers Linkage Map in Tea Plant HUANG Jian-an, LI Jia-xian, HUANG Yi-huan, LUO Jun-wu,GONG Zhi-hua, LIU Zhong-hua Journal of Tea Science    2005, 25 (1): 7-15.   DOI: 10.13305/j.cnki.jts.2005.01.002 Abstract （334）      PDF（pc） （962KB）（150）       Knowledge map    Save Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map used a set of 69 F1 progenies of Qi Men No.4×Chao An Da Wu Ye as a mapping population. In this work, all together 1925 bands were obtained by 22 pairs of primers and 87.5 bands each primer pair on average, a total of 485 (25.19%) polymorphic AFLP markers were detected. The 356 (73.40%) of polymorphic markers with Mendelian segregation ratio (P=0.01) were obtained. 247（69.38%）of the 356 loci were 1:1 segregation, 109（30.62%）of the 356 loci were 3:1 segregation. Furthermore, the AFLP molecular markers linkage maps of Qi Men No.4 and Chao An Da Wu Ye were constructed respectively by using Mapmaker/Exp (version3.0). The map of female parent included 17 linkage groups and located 208 markers, covered total map length of 2457.7 cM, and average distance between markers was 11.9 cM. The map of male parent included 16 linkage groups and located 200 markers,covered total map length of 2545.3 cM, and average distance between markers was 12.8 cM. Reference | Related Articles | Metrics Cited: Baidu(68) Select Research Progress on Resistance Breeding of Tea Plant WANG Xin-chao，YANG Ya-jun Journal of Tea Science    2003, 23 (2): 94-98.   DOI: 10.13305/j.cnki.jts.2003.02.002 Abstract （362）      PDF（pc） （385KB）（206）       Knowledge map    Save This paper summarized the progress of cold hardness resistance、drought resistance、resistance to disease and pest on tea breeding in china and abroad，and put forward some further research focal points on these aspects. Reference | Related Articles | Metrics Cited: Baidu(62) Select Elite Germplasm Evaluation and Genetic Stability of Tea Plants YANG Ya-jun, YU Fu-lian, CHEN Ling, ZENG Jian-ming, YANG Su-juan, LI Su-fang, SHU Ai-ming, ZHANG Zhi-fang, WANG Yu-shu, WANG Hai-si, WANG Ping-sheng, XU Mei, SONG Wei-xi, GUO Ji-chun, YANG Ru-xing, ZHANG Wen-jin, CHEN Zhi-hui Journal of Tea Science    2003, 23 (s1): 1-8.   DOI: 10.13305/j.cnki.jts.2003.s1.001 Abstract （282）      PDF（pc） （330KB）（193）       Knowledge map    Save The agronomy, made tea quality, chemical components, cold resistance of 200 accessions of tea germplasms were investigated during 1996-2000. Among six accessions of elite tea germplasms, their made tea quality exceeded national clones, four with high polyphenol content (>38%), six with high caffeine content (>5.2%) and two with low caffeine content (<1%) germplasms were selected. Based on the studies of RAPD marker, isozyme and pollen morphology, the heredity of tea germplasms and their cutting offsprings was basically stable. Reference | Related Articles | Metrics Cited: Baidu(60) Select Effect of Tea Polysaccharides on Immune Functions and Antioxdative Activity in Broilers HU Zhong-ze, JIN Guang-ming, WANG Li-ke, YANG Jiu-feng Journal of Tea Science    2005, 25 (1): 61-64.   DOI: 10.13305/j.cnki.jts.2005.01.011 Abstract （254）      PDF（pc） （312KB）（141）       Knowledge map    Save To study the effect of tea polysaccharide on immune function and antioxidative activity in broilers, 0%、0.2%、0.4% of tea polysaccharide were added into the drinking water for broilers respectively. Results showed that thymus index and serum IgG levels of broilers were increased obviously by TP（P<0.05）, furthermore lymphocyte transformation rate, macrophage activity, percentage of T cell numbers Serum SOD, GSH-Px and CAT activities in broilers were increased obviously by TP at same time（P<0.05）but not to index of fabricius bursa of broilers. Serum MDA levels of broilers were declined obviously by TP（P<0.05）. Reference | Related Articles | Metrics Cited: Baidu(60) Select The Bioactivities and Synthesis of Theanine LU Yi, GUO Wen-fei, NI Jie-er, YANG Xian-qiang Journal of Tea Science    2003, 23 (1): 1-5.   DOI: 10.13305/j.cnki.jts.2003.01.001 Abstract （408）      PDF（pc） （451KB）（252）       Knowledge map    Save The recent researches have found that theanine, an unique and important component in tea plants, has many bioactivities, and made it get more and more attention. The property, bioactivities, and industrial preparation of theanine are reviewed. Reference | Related Articles | Metrics Cited: Baidu(58) Select Effect of Dietary Tea Polyphenols on Performances, Lipid Metabolism and Egg Quality of Laying Hens LOU Hong-xing, LIN Zi, WANG You-ming, LU Fu-zhuang, TANG Jun-feng, YING Jun-feng, YANG Zhong-ming Journal of Tea Science    2004, 24 (2): 135-140.   DOI: 10.13305/j.cnki.jts.2004.02.012 Abstract （374）      PDF（pc） （283KB）（187）       Knowledge map    Save There were two feeding trials in this research. 225 37-week-old Lohamann laying hens were randomly divided into five treatments for trial one,fed a basal diet supplemented with 0, 0.05, 0.1, 0.2, 0.4 percentage tea polyphenols(TP) for a period of 59 days, respectively.600 51-week-old Lohamann laying hens were randomly divided into four treatments for trial two, fed a basal diet added with 0, 0.025, 0.05, 0.1 percentage TP for a period of 8 weeks, respectively. Dietary effects of TP supplementation on performance, lipid metabolism and egg quality were investigated in laying hens. The results showed that: (1) Egg production and feed conversion efficiency were improved to a certain extent by supplementing TP, but they were not improved with the increasing levels of added TP. (2) The addition of 0.1% TP in diet increased egg yolk VE and VA concentration by 58.99% and 20.96%, respectively. And TP was tended to increase whole egg amino acid levels containing S. The supplementation of 0.025%—0.1% TP in diet of layers at later period decreased broken and soft-shelled egg rates by 31.59%—47.09%. (3) The addition of 0.4% TP in diet decreased total cholesterol (TCH), triglyceride(TG), LDL-C contents of layer serum with 46 week old by 19.65%, 19.99% and 7.69%, respectively, and increased HDL-C by 45.35%. No significant change of 0.025%—0.1% TP supplementation on serum lipid levels was found in layers with 59βw old. Reference | Related Articles | Metrics Cited: Baidu(58) Select Analysis on Applicability of ISSR in Molecular Identification and Relationship Investigation of Tea Cultivars YAO Ming-zhe, HUANG Hai-tao, YU Ji-zhong, CHEN Liang Journal of Tea Science    2005, 25 (2): 153-157.   DOI: 10.13305/j.cnki.jts.2005.02.013 Abstract （377）      PDF（pc） （379KB）（191）       Knowledge map    Save Inter-simple sequence repeat (ISSR) is a recently developed PCR-based molecular technology. It has been widely used in genetic diversity, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of crop species due to its simple, quick, efficient, high reproducibility and low expense. In this study, the applicability of ISSR on molecular identification and relationship analysis of tea cultivars were investigated. A total of 99 bands were amplified by eight ISSR primers in six tea cultivars and the average polymorphism was 79.6%. The six tea cultivars could be clearly distinguished by ISSR fingerprinting amplified with primers TRI22 and TRI30, and also by the presence or absence of specific ISSR markers and their combination. Six cultivars were divided into two groups by cluster analysis. Maolu, Cuifeng, Qingfeng, Yingshuang and Jinfeng were in one group and Longjing 43 in the other. The relationship of six tea cultivars were validated by cluster analysis based on ISSR. The results indicate that ISSR is a useful tool for molecular identification and relationship analysis of tea cultivars. Reference | Related Articles | Metrics Cited: Baidu(56) Select Study on the Hypoglycemic Mechanism of Tea Polysaccharides and Tea Polyphenols DING Ren-feng, HE Pu-ming, JIE Guo-liang Journal of Tea Science    2005, 25 (3): 219-224.   DOI: 10.13305/j.cnki.jts.2005.03.011 Abstract （459）      PDF（pc） （399KB）（202）       Knowledge map    Save The investigation on the hypoglycemic mechanism of tea polysaccharides and tea polyphenol in diabetic SD rats induced by alloxan was conducted. Methods: After feeding tea polysaccharides or polyphenols for 3 weeks, the changes of the level of blood glucose, insulin and the activities of amylase, sucrase and maltase in small intestine were inspected in diabetic rats. Results about that the suppressive effect of high level of blood glucose induced by alloxan was observed in the groups feeded with tea polysaccharides or tea polyphenols obviously in the experimental period. Compared with control group, the level of blood insulin in the group feeded with tea polysaccharides was increased significantly (P<0.05), the activity of sucrase and maltase were decreased significantly. Almost the same change without significance was observed in the group feeded with tea polyphenols while compared with the control group. Investigation indicated that the tea polysaccharides and tea polyphenols have remarkable suppressive hyperglycemic effect on diabetic rats induced by alloxan. These results suggested that functionary mechanisms of the suppressive hyperglycemic effect of tea polysaccharides may be induced by controlling the activities of sucrase and maltase. Reference | Related Articles | Metrics Cited: Baidu(55) Select Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers Rajan Kumar Mishra, Swati Sen-Mandi Journal of Tea Science    2004, 24 (2): 86-92.   DOI: 10.13305/j.cnki.jts.2004.02.003 Abstract （283）      PDF（pc） （292KB）（115）       Knowledge map    Save Tea plants growing in Darjeeling area, in addition to all other factors described below, are unique due to their typical geographical isolation. DNA fingerprints revealed a high degree of polymorphism in genomes of different clones, and this demonstrated the suitability of using the Amplified Fragment Length Polymorphism (AFLP) method for genome analysis between closely related plants, such as vegetatively propagated (clonal) populations of tea. Cluster analysis exhibited a dendrogram that closely matched with earlier clonal grouping based on morphological characters. The extent of genetic relatedness between the clones was found to be at 70% level. Results also showed that the genetic variation (Hs) was higher among China type. The variation between and within the three types of clonal populations studied (viz. China, Assam and Cambod type) are 63% and 36% respectively. Reference | Related Articles | Metrics Cited: Baidu(54) Select Effect of Cold Acclimation and ABA on Cold Hardiness, Contents of Proline in Tea Plants YANG Ya-jun, ZHEN Lei-ying, WANG Xin-chao Journal of Tea Science    2004, 24 (3): 177-182.   DOI: 10.13305/j.cnki.jts.2004.03.006 Abstract （395）      PDF（pc） （158KB）（156）       Knowledge map    Save Effect of cold acclimation and ABA on cold hardiness resistance, contents of proline in tea plants was studied. The results showed that through cold acclimation and ABA treatment, the cold hardiness of tea plants was changed greatly during the process of acclimation to deacclimation, showing a trend from low to high then to low again. And ascertain 7℃and 9℃are the crucial temperature to finish acclimation or start declimation respectively.On the other hand, it can be concluded that the rising degree of cold hardiness resistance was lower during artificial or ABA treatment than that of natural cold acclimation. At the same time, changes of Proline content weren’t regular,the relationship between proline content and cold hardiness resistance was insignificant. It implied that changes of proline content during cold acclimation were an integrative response in tea plant. Reference | Related Articles | Metrics Cited: Baidu(53) Select Determination of the Total Content of Polyphenols in Kudincha by Folin-Ciocalteu Colorimetry LIU Li-xiang, Tanguy Laura, LIANG Xing-fei, SUN Yi, YE Hong, ZENG Xiao-xiong Journal of Tea Science    2008, 28 (2): 101-106.   DOI: 10.13305/j.cnki.jts.2008.02.006 Abstract （389）      PDF（pc） （236KB）（497）       Knowledge map    Save A quantitative method for the determination of the total content of polyphenols in kudincha was studied by using FC method (Folin-Ciocalteu colorimetry ) with chlorogenic acid as standard. The results showed that the total polyphenol contents of kudincha could be well calculated according to their colorimetric absorption at 747 nm by applying Folin-Ciocalteu reagent 3.0 ml and saturated Na2CO3 4.5 ml at 30℃ for 30 min, and the linear range of standard curve was 20.0~100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0.887%~1.416% by RSD (relative standard deviation). There are not any differences for the results obtained by FC method and ferrous tartrate colourimetry. Therefore, FC method is convenient, fast, and accurate to determine the total polyphenols of kudincha. Reference | Related Articles | Metrics Cited: Baidu(51) Select Genetic Mapping of First Generation of Backcross in Tea by RAPD and ISSR Markers HUANG Fu-ping, LIANG Yue-rong, LU Jian-lian, CHEN Rong-bing Journal of Tea Science    2006, 26 (3): 171-176.   DOI: 10.13305/j.cnki.jts.2006.03.003 Abstract （267）      PDF（pc） （545KB）（160）       Knowledge map    Save The segregation mode for 94 plants in first generation of back cross (BC1) of tea cultivar Fudingdabaicha were investigated using 14 RAPD primers and 20 ISSR primers. One hundred and seventy four segregation loci was identified. Among the 174 loci, 90 loci or 51.7% were segregated at the expected Mendelian ratio of 1:1, in which 63 belonged to ISSR loci and 27 belonged to RAPD loci, and 36 loci segregated in the ratios of 3:1 or 1:3. Sixty-two of the 126 loci with segregation ratios of 1:1, 3:1 and 1:3 were included in 7 linkage groups through linkage mapping by Mapmaker EXP 3.0 software and the other 64 loci were not included in the 7 linkage groups because of their far genetic distance. This map concluded 46 RAPD markers and 16 ISSR markers, covering distance of 1180.9βcM, with average distance of 20.1βcM. The linkage group LG4 covered the farthest genetic distance with 309.3βcM and group LG6 had the largest number of markers, with 18 loci and its average distance being 15.7βcM. Reference | Related Articles | Metrics Cited: Baidu(49) Select The Improvement of Isolation and The Method of Two-Dimensional Electrophoresis of Protein from Tea Plant LIN Jin-ke, ZHENG Jin-gui, YUAN Ming, ZHANG Xue-qin, WANG Feng Journal of Tea Science    2003, 23 (1): 16-20.   DOI: 10.13305/j.cnki.jts.2003.01.004 Abstract （290）      PDF（pc） （700KB）（181）       Knowledge map    Save Reference | Related Articles | Metrics Cited: Baidu(49) Select Study on the Characteristic of Aroma Components in Green Tea with Chestnut-like Aroma YE Guo-zhu, JIANG Yong-wen, YIN Jun-feng, YUAN Hai-bo, ZHANG Rui-lian, WANG Zhi-lan, SHEN Dan-yu, WANG Fang, CHEN Jian-xin Journal of Tea Science    2009, 29 (5): 385-394.   DOI: 10.13305/j.cnki.jts.2009.5.009 Abstract （442）      PDF（pc） （570KB）（200）       Knowledge map    Save E Mei MaoFeng tea with chestnut-like aroma was processed under different temperature and time to change the intensity and type of the aroma. The treated teas and E Mei MaoFeng tea were classified into two types—teas with chestnut-like aroma and others without chestnut-like aroma, according to the organoleptic evaluation. After analysis on the differences of the two types of teas using Duncan’s multiple tests, principal component analysis was applied to observe and analyze the distribution of the selected aroma components and the tea samples. The results demonstrated that the teas with chestnut-like aroma included remarkably high contents of β-ionone, nerolidol, phytol, 1,4-eicosadiene, 5,8,11,14-eicosatetraenoic acid, ethyl ester, (all-Z)-, 2,6-di-tert-butylbenzoquinone, pentadecane,2-methyl-, heptadecane, hexadecane,2,6,10-trimethyl-, but the content of dodecanol,3,7,11-trimethyl- was remarkably lower than the later. The ten aroma components have a good effect of distinguishing the teas with chestnut-like aroma from those without chestnut-like aroma. On the other hand, nonanal, and cis-jasmone are also worthy of attention. Reference | Related Articles | Metrics Cited: Baidu(49) Select Construction of Tender Shoots cDNA Library and Preliminary Analysis of Expressed Sequence Tags Sequencing of Tea Plant CHEN Liang, ZHAO Li-ping, GAO Qi-kang Journal of Tea Science    2004, 24 (1): 18-22.   DOI: 10.13305/j.cnki.jts.2004.01.003 Abstract （386）      PDF（pc） （423KB）（174）       Knowledge map    Save The construction of the first cDNA library of tea plant [Camellia sinensis cv. Longjing 43] in China and the analysis of expressed sequence tags sequencing successful ratio were reported. Total RNA was isolated from tender tea shoots using TRIZOL single-step method, mRNA separated and then the double-strand cDNA amplified by LD-PCR. After size fractionation, the ds-cDNA was ligated to λTripEX2 and recombinant bacteriophages were packaged. The cDNA library was tittered and amplified using XL1-Blue as receptor bacterium. The titer of the original library was 6.8×105pfu/ml with a recombinant rate of 98.05% and 3.5×105 clones in total, the amplified titer was 7.2×109pfu/ml. PCR amplification suggested the inserted cDNA fragments ranged from 0.5 kb to 2 kb, mostly from 1.0 kb to 1.5 kb. The data indicate that the tea cDNA library has high titer, high recombinant percentage and large inserted fragments. Totally, 4320 clones were sequenced, 2963 useful sequences were obtained, corresponding to 68.5%. The first batch of 1687 valid tea plant ESTs were generated. Reference | Related Articles | Metrics Cited: Baidu(49) Select Dynamic Change of Main Biochemical Components of Premium Green Tea Fresh Leaves during Spreading YIN Jun-feng, XU Yong-quan, YUAN Hai-bo, YU Shu-ping, WEI Kun-kun, CHEN Jian-xin, WANG Fang, WU Rong-mei Journal of Tea Science    2009, 29 (2): 102-110.   DOI: 10.13305/j.cnki.jts.2009.2.004 Abstract （409）      PDF（pc） （289KB）（346）       Knowledge map    Save By using of a leaf and a bud shoot as fresh tea leaves material, and spread continuously (78%~61%), spread leaves with different moisture content were fixed with liquid nitrogen and freeze-dried. then the contents of tea polyphenols and catechin components, total amino acids and free components, caffeine, total chlorophyll, Vc and total soluble sugar were determined to study the dynamic change of biochemical components of fresh tea leaves during spreading. Results showed that with increasing of spreading time, moisture content of fresh tea leaves decreased rapidly gradually, weight of dry substance decreased; contents of tea polyphenols and total catechins decreased at first and then increased during spreading, total esters-catechins decreased gradually, but GC could not be detected in fresh tea leaves and spread tea leaves, content of total amino acids increased, but different free amino acid have different changing trends during spreading; content of caffeine and total soluble sugar increased while total chlorophyll and Vc decreased gradually. Different biochemical components of fresh tea leaves changes in different rules during spreading. This study provides supplied some theory for improving the quality of premium green tea. Reference | Related Articles | Metrics Cited: Baidu(48) Select Effects on the Yield and Quality of Oolong Tea by Covering with Shading Net Journal of Tea Science    2004, 24 (4): 276-282.   DOI: 10.13305/j.cnki.jts.2004.04.010 Abstract （289）      PDF（pc） （87KB）（164）       Knowledge map    Save The effects of shading on ecology, physiology and biochemistry of Oolong tea by covering with black shading net in relative tea field in summer season were investigated by the authors. Results showed that in compared with control, the average temperature of daytime, maximum temperature, temperature difference and the relative humidity difference in plucking table of tea plant had been decreased remarkably, while the relative humidity of daytime and the daily minimum relative humidity in plucking table of tea plant in the shading treatment were remarkably increased. In compared with control, moisture content of shoots with one bud and three-leaves in the treatment of shading in summer and hot summer day was increased by 4.57％~6.47％ and 2.24％~2.78％ respectively. The content of total chlorophyll in leaves of the tea shoot in summer and hot summer day were respectively increased by 41.70％~48.92％and 52.38％~91.06％over the control. Similarly, chlorophyll a by 38.96％~47.25％ and 50.94％~72.52％,while chlorophyll b by 43.83％~54.58％and 67.98％~155.81％ were increased. Whereas the ratio of chlorophyll a and b was decreased by 0.1042~0.1868 and 1.10~3.54 respectively. After shading, the leaf epidermis, cuticle, palisade tissue, spongy tissue was thinned, while the lamina area was decreased, and the ratio of palisade tissue to spongy tissue was decreased. After shading, the contents of tea polyphenols, crude cellulose and caffeine in fresh leaves are all obviously reduced, while the contents of amino acid in fresh leaves are obviously increased. It was regarded that moderate shading could improve the quality and the yield of Oolong tea. Reference | Related Articles | Metrics Cited: Baidu(45) Select Biochemical and Molecular Biological Basis on The Anticarcinogenic Activity of Tea Polyphenolic Compounds CHEN Zong-mao Journal of Tea Science    2003, 23 (2): 83-93.   DOI: 10.13305/j.cnki.jts.2003.02.001 Abstract （417）      PDF（pc） （496KB）（460）       Knowledge map    Save The progress in the investigation of anticarcinogenic activity of tea polyphenols and its biochemical and molecular biological mechanisms in the world in recent 5 years were analyzed and reviewed in this paper. The mechanism of anticarcinogenic activity was discussed from the following 5 aspects: antioxidative activity, modulation on the key enzymes, blockage of signal transduction, antiangiogenic activity and the apotopsis of cancer cells. The developmental prospect and the investigational tendency of tea polyphenols in the future were discussed. Reference | Related Articles | Metrics Cited: Baidu(44) Select Effect of Drying Methods on the Aromatic Character of Pu-erh Tea ZHANG Ling-zhi, CHEN Wei-xin, WANG Deng-liang, ZHOU Li-min, CHEN Yu-fen Journal of Tea Science    2007, 27 (1): 71-75.   DOI: 10.13305/j.cnki.jts.2007.01.011 Abstract （365）      PDF（pc） （374KB）（227）       Knowledge map    Save With the aim to study the effect of drying methods on the aroma Characteristics of Pu-erh tea, the headspace solid-phase microextraction (HS-SPME) coupled with capillary gas chromatography-mass spectrometry (GC-MS) was used for the determination of volatiles in Pu-erh tea dried by baking, air-drying and sun-drying respectively. Results showed: Thirty-five compounds representing 95.4% of aroma contents of air-dried sample were identified with lower contents of terpenes and alcohols but higher contents of esters. Some biphenyls compounds which contributed to the stale odor of Pu-erh tea were distinct compounds of the air-dried sample. As for the sun-dried sample, Twenty-eight compounds representing 96.5% of aroma contents were identified with higher contents of ketones and lower contents of esters. In the baked sample, Twenty-four compounds representing 98.6% of aroma contents were identified with more alcohols compounds such as epoxylinalol, α-terpineol, 2-hydroxy-3,5,5-trimethyl-cyclohex- 2-enone contributed to the camerlsed and sweet flower odor of tea. The results were consistent with that of sensory tests. Sensory tests showed: The characteristic aroma of sun-dried tea, baked tea and air-dried tea were pungent, high-fired and stale odour, respectively. Results showed both baking methods and sun-drying methods were not suitable for Pu-erh tea, but the air-drying methods were the best method of the formation of Pu-erh tea aroma. Reference | Related Articles | Metrics Cited: Baidu(44) Select Study on the Hypolipidemic Effect of Flavones and Dihydromyricetin From Tengcha CHEN Yu-qiong, NI De-jiang, CHENG Qian, HUANG Hai-bo, MENG Yan, WU Mou-cheng Journal of Tea Science    2007, 27 (3): 221-225.   DOI: 10.13305/j.cnki.jts.2007.03.015 Abstract （343）      PDF（pc） （593KB）（212）       Knowledge map    Save The effects of Tengcha flavones and dihydromyricetin(DMY) on hypolipidemic mice were investigated. The results showed, compared with the hyperlipidemia control group, DMY reduced the weights of mice significantly; Tengcha flavones and DMY decreased serum TC、TG、LDL-C、malondiadehyde(MDA) contents and Liver coefficient, increased HDL-C levels and the activities of SOD distinctly. The pathological observation on the liver tissue showed that Tencha flavones and DMY could lighten the denaturation liver cell. As results, Tengcha flavones and DMY could prevent hypolipidemia in mice and protect myocardial cells from oxidation and the liver harmful from of high lipid. Reference | Related Articles | Metrics Cited: Baidu(43) Select Aroma Components of Oolong Tea by HS-SPME-GC-MS and GC-O MIAO Ai-qing, LU Hai-peng, SUN Shi-li, WANG Li, PANG Shi, LAI Zhao-xiang, ZENG Qiong, LIN Zhi Journal of Tea Science    2010, 30 (S1): 583-587.   Abstract （282）      PDF（pc） （244KB）（208）       Knowledge map    Save The aroma components of four Oolong teas were estimated by HS-SPME-GC-MS and GC-O. The results showed that the main volatile aroma components of Baiye Dancong were linalool and its oxide (I), indoles for Jinxuan, linalool, methyl salicylate, (Z)-hexanoic acid-3-hexenyl ester and alpha-farnesene for Tieguanyin, and linalool oxide (I), (Z)-hexanoic acid-3-hexenyl ester and alpha-farnesene for Huangjingui, respectively. Reference | Related Articles | Metrics Cited: Baidu(42) Select Effect of Chemical Insecticides on the Diversity of Arthropods in Tea Garden WANG Guo-hua, LIANG Yuan-fa, TIAN Yong-hui, WANG Jia-lum, ZHOU Guo-lan Journal of Tea Science    2004, 24 (2): 99-104.   DOI: 10.13305/j.cnki.jts.2004.02.005 Abstract （374）      PDF（pc） （386KB）（148）       Knowledge map    Save The effect of chemical insecticides on the structure and diversity of arthropods in various tea gardens were investigated. Results showed that the effect of chemical insecticides on the arthropods in tea gardens reflected in the structure and the constitution of species, the diversity index and the distributive evenness of species. The index of population density of dominant insect pest was increased greatly in those tea gardens with higher chemical insecticide expense, especially for tea leaf hopper (Empoasca vitis Gotho). The regulatory effect of natural enemies on major insect pest was increased in those tea gardens with lower expenses of insecticides. Besides, the diversity index and the evenness index were increased at the same time. Reference | Related Articles | Metrics Cited: Baidu(41) Select Extraction of Tea-polyphenols from Tea Leaves by Ultrahigh Pressure Technique ZHANG Ge, ZHANG Ling-ling, WU Hua, ZHANG Shou-qin Journal of Tea Science    2006, 26 (4): 291-294.   DOI: 10.13305/j.cnki.jts.2006.04.011 Abstract （456）      PDF（pc） （326KB）（290）       Knowledge map    Save The extactrion of tea-polyphenols from tea leaves by using the ultrahigh pressure extraction technology (UPE) was studied. The experiment was designed with orthogonal method, and the yield of tea-polyphenols is used as the testing index. The extract conditions, including of the solvent concentration, pressure, the holding time of pressure and the soaking time before extraction, were investigated. The optimum extraction conditions are as follow, 60% ethanol, 200βMPa holding 3βmin., soaking 0.5 hr. The yield of tea-polyphenols is 28.92% under the optimum extraction condition. After separation, the yield of crude product of tea extraction is higher 25.3% than that of heat reflux method and the total of tea-polyphenols is higher 32% than that of heat reflux method. In addition, the extraction liquid of UPE is clear, easily to filter and no turbid after stored a long time. As a new extraction technology, UPE showed the great economic benefit if applied to extract tea-ployphenols from tea leaves in industrial scale. Reference | Related Articles | Metrics Cited: Baidu(40) Select Study on Chemistry and Bioactivities of Tea Polysaccharides XU Zhong-xi, WANG Kun-bo Journal of Tea Science    2004, 24 (2): 75-81.   DOI: 10.13305/j.cnki.jts.2004.02.001 Abstract （307）      PDF（pc） （280KB）（485）       Knowledge map    Save The recent researches have found that tea polysaccharide has many bioactivities, such as anti-thrombosis, reducing blood-sugar, blood-lipid and pressure depressing and strengthening the body's immunity. It can be used in curing diabetes and cardiovascular disorder as healthy foods and subsidiary medicine. The methods of extraction and purification, determination of purity and molecular weight, chemical composition, and bioactivities of tea polysaccharide are summarized. Reference | Related Articles | Metrics Cited: Baidu(39) Select Comparative Analysis on Aromatic Components of Green Tea and Fresh Green Tea Beverage Using HS-SPME/GC-MS/GC-Olfactometry/RI Methods DOU Hong-liang, LI Chun-mei, GU Hai-feng, HAO Ju-fang Journal of Tea Science    2007, 27 (1): 51-60.   DOI: 10.13305/j.cnki.jts.2007.01.008 Abstract （376）      PDF（pc） （501KB）（205）       Knowledge map    Save ：Volatile components of green tea and fresh green tea berverage was extracted using HS-SPME and the major flavor compounds were identified by GC-MS/GC-Olfactometry/RI. The difference of aromatic constituents in composition and relative content between green tea and fresh green tea beverage was also compared.The results showed that the sort、intensity and contribution to total aroma of an aromatic compound could be identified and affirmed effectively by GC-MS/GC-Olfactometry/RI methods; The aromatic constituents of green tea were different from fresh green tea beverage in composition and relative content. Green tea contained 34 kinds of smell compounds, in which 3-Hexen-1-ol, 2-Ethyl-1-hexanol, Benzylalcohol, 1-Octanol, linalool, cis-Linalool oxide, Phenylethyl alcohol, Hexanal, (E)-2-Hexenal, Benzaldehyde, Acetophenone et al were the primary aromatic compounds. Fresh green tea beverage contained 37 kinds of smell compounds, in which linalool, trans-Linalool oxide, Phenylethyl alcohol, Geraniol, Nerol, Nerolidol, (Z)-2-Heptenal, Benzaldehyde, Acetophenone were the essential aromatic constituents. Reference | Related Articles | Metrics Cited: Baidu(38) Select Establishment of ISSR-PCR Reaction Conditions in Tea Plant YAO Ming-zhe, WANG Xin-chao, CHEN Liang, YANG Ya-jun Journal of Tea Science    2004, 24 (3): 172-176.   DOI: 10.13305/j.cnki.jts.2004.03.005 Abstract （380）      PDF（pc） （156KB）（155）       Knowledge map    Save The optimal ISSR-PCR reaction conditions in tea was established by studying the main parameters. Results showed that the optimum concentration of five important components i.e. template DNA, primer, Mg++, dNTP, Taq DNA polymerase in 20βµl reaction mixture was 10ng, 150βnmol/L, 1.5βmmol/L, 150βµmol/L, 0.5βU, respectively. The appropriate annealing temperature was average 4.5℃ higher than Tm of corresponding primer, and at least 30 PCR cycles should be carried out to ensure sufficient PCR products. ISSR polymorphism between thirteen tea germplasm was 77.6%, and 12 out of 13 tea germplasm could be identified by ISSR fingerprinter established with primer TRI18. Reference | Related Articles | Metrics Cited: Baidu(37) Select Studies on the Quality Chemical Components in Sichuan Brick Tea QI Gui-nian, TIAN Hong, LIU Ai-ling, SHI Zhao-peng Journal of Tea Science    2004, 24 (4): 266-269.   DOI: 10.13305/j.cnki.jts.2004.04.008 Abstract （290）      PDF（pc） （33KB）（158）       Knowledge map    Save The contents and constituents of amino acids, catechins, caffeine, tea polyphenols and water extracts in raw material, fermented leaves, Kang Zhuan tea from Sichuan province were studied using amino acid auto-analysis and HPLC analysis. The content of amino acids, essential amino acids, theanine,catechins, caffeine, tea polyphenols and water extracts in raw materials were 1424.00βmg/100g, 547.00βmg/100g, 87.15βmg/100g, 27.63βmg/g, 1.30%, 8.18% and 26.94%,respectively; those in fermented leaves were 1590.00βmg/100g, 668.00βmg/100g, 67.62βmg/100g. 27.52βmg/g, 1.24%, 7.90% and 24.53% respectively, and those in Kang Zhuan tea were 1420.00βmg/100g, 529.00βmg/100g, 66.88βmg/100g, 13.65βmg/g, 1.27%, 5.99% and 23.92%, respectively. Reference | Related Articles | Metrics Cited: Baidu(37) Select Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction JI Peng-zhang, ZHANG Jun, WANG Ping-sheng, HUANG Xing-qi, XU Mei, TANG Yi-chun, LIANG Ming-zhi Journal of Tea Science    2007, 27 (4): 271-279.   DOI: 10.13305/j.cnki.jts.2007.04.001 Abstract （415）      PDF（pc） （544KB）（242）       Knowledge map    Save Tea plant [Camellia sinensis var. assamica] populations in Yunnan province of China cultivated since ancient times are important genetic resources for future breeding of new tea cultivars. To evaluate the genetic diversity within and between Yunnan ten representative populations and to formulate conservation strategies for the ancient tea populations in Yunnan was conducted by ISSR technique in this study. The genetic diversity within populations calculated from Nei's genetic diversity (He) was estimated to be 0.281, on average, at the population level and 0.461 at the variety level, while Shannon indices (Ho) of population level and Assam variety level were 0.418 and 0.653, respectively and the percentage of polymorphic loci (P) of the ten populations ranged between 56.5% to 90.9%, respectively. Coefficients of gene differentiation (Gst) within the population was 0.391, in agreement with the result of Shannon’ diversity indices analysis (36.0%) and analysis of molecular variance (AMOVA) among population (39.7%), which illuminated that 60.9% of the variation in Assam variety were from the individuals of the population, and 39.1% of the genetic variation were from the population. The result indicated Camellia var. assamica variety possesses high genetic diversity and medium genetic differentiation was existed in the genetic variation between population. This could be caused by the highly outcrossing nature and habitat fragmentation of tea species. Conservation strategy are suggested including in situ strategies and ex situ strategies based on the observed genetic information of population. Reference | Related Articles | Metrics Cited: Baidu(36) Select Study on the Aroma Components in Pu-erh Tea with Stale Flavor LU Hai-peng, ZHONG Qiu-sheng, LIN Zhi Journal of Tea Science    2009, 29 (3): 219-224.   DOI: 10.13305/j.cnki.jts.2009.3.007 Abstract （449）      PDF（pc） （239KB）（249）       Knowledge map    Save Five Pu-erh tea samples with stale flavor were selected from 36 samples by sensory tests, and the aroma constituents of these samples were determined by HS-SPME/GC-MS. Furthermore the mainly difference in aroma constituents between Pu-erh tea with stale flavor and other Pu-erh tea samples was discussed in the paper. Results showed that the aroma constituents of Pu-erh tea with stale flavor were mainly heterocyclic oxygen compounds and alcohols, and the sum of the two mentioned above occupied 40% of the total, the major aromatic compounds of Pu-erh tea with stale flavor were 1,2,3-Trimethoxybenzene, Benzene,4-ethyl-1,2-dimethoxy-,β-Terpineol, Epoxylinalol, Linalool oxideⅠ, 3,4-Dimethoxytoluene, Benzene, 1,2-dimethoxy-, Cedrol, Butylated Hydroxytoluene, Benzene, 1,2,3-trimethoxy-5-methyl-, β-Linalool, 1,2,4-Trimethoxybenzene, β-Ionone, α-Farnesene. The contents of β-Linalool, Decanal, Nonanal, Methyl Salicylate, 3,4-Dimethoxytoluene, Benzene, 4-ethyl-1,2-dimethoxy-, Butylated Hydroxytoluene were much higher than that of other Pu-erh tea samples. However, the contents of β-Terpineol, Cedrol, β-ionone, 1,2,4-Trimethoxybenzene, Benzene, 1,2-dimethoxy-, 1,2,3-Trimethoxybenzene and Longifolene were much lower than that of other Pu-erh tea samples. Reference | Related Articles | Metrics Cited: Baidu(36) Select Genetic Diversity and Relationship of Huangjincha Cultivar Based on EST-SSR Markers YANG Yang, LIU Zhen, ZHAO Yang, LIANG Guo-qiang, ZHAO Xi Journal of Tea Science    2009, 29 (3): 236-242.   DOI: 10.13305/j.cnki.jts.2009.3.010 Abstract （327）      PDF（pc） （347KB）（206）       Knowledge map    Save The 19 pairs of EST-SSR primers were used to analyze the genetic polymorphism and genetic relationship of 38 Huangjincha individuals. Totally 37 alleles were amplified using 19 EST-SSR primers, the number of alleles per primer ranged from 1 to 3, on average of 1.95. The expected heterozygosity (He) varied from 0.03 to 0.65, and the observed heterozygosity (Ho) ranged from 0 to 0.97, on average of 0.32 and 0.33, respectively. The Shannon index of the Huangjincha was 0.55. All of the results showed that the genetic polymorphism of Huangjincha was relatively poor. The 38 individuals were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.77. The results of the similarity coefficient among different individuals can give some implications to the genetic improvement of Huangjincha. At the same time, analysis of molecular variance (AMOVA) showed that variance component among groups and within populations were 21.77% and 78.23% each other, gene flow among the groups is 1.80, which all can give us some supports to protect the Huangjincha. Reference | Related Articles | Metrics Cited: Baidu(36) Select Effect of Exogenous Methyl Jasmonate-induced Tea Volatiles on Host-selection Behavior of Insects GUI Lian-you, CHEN Zong-mao, LIU Shu-sheng Journal of Tea Science    2004, 24 (3): 166-171.   DOI: 10.13305/j.cnki.jts.2004.03.004 Abstract （311）      PDF（pc） （46KB）（169）       Knowledge map    Save The effects of volatiles from tea plants induced by exogenous methyl jasmonate (MJA) spraying or MJA-exposure on host-selection behavior of the parasitoid Apanteles sp. and the geometrid Ectropis obliqua as well as the percentage of E. obliqua caterpillars parasitized by Apanteles sp. under field condition were studied. Tea volatile organic compounds (VOC) induced by exogenous MJA-spraying or MJA-exposure exhibited a significant increase of attractiveness to female adults of Apanteles sp., but not to female adults of E. obliqua; and also significantly increased the percentage of E. obliqua caterpillars parasitized by Apantele sp in field condition. Reference | Related Articles | Metrics Cited: Baidu(35) Select Chemical Communication Between Tea Plant-Herbivore-Natural Enemies CHEN Zong-mao, XU Ning, HAN Bao-yu, ZHAO Dong-xiang Journal of Tea Science    2003, 23 (s1): 38-45.   DOI: 10.13305/j.cnki.jts.2003.s1.008 Abstract （414）      PDF（pc） （435KB）（171）       Knowledge map    Save The relation of chemical communication between the host-herbivore-natural enemies is the basis of existence of the member in the food chain. The chemical communication between tea plant and the three major insect pests (tea geometrid, tea aphid, tea leafhopper) as well as the seven natural enemies was investigated by the authors. Results indicated that the location of insect pest on the tea plant depended on the volatiles emitted from the tea shoots, such as the z-3-hexen-1-ol, linanool, n-pentanol etc. Bioassay and EAG estimation showed that these compounds showed strong attractiveness and electrophysiological response to tea pests, however, they showed only a weak activities to the natural enemies. The metabolism in tea plants was changed and released the specific synomone after damaged by the pests. For example, the damage of tea geometrid induced more C5-C6 aldehyde compounds liberated and tea aphid damage induced the liberation of benzyaldehyde. The damage of tea shoots by tea leafhopper induced the release of 2,6-dimethyl-3,7-octadien-2,6-diol and indole. These compounds showed a strong attractiveness and electro-physiological response to their respective natural enemies under a very low concentration of 10-6-10-9βg/ml, however, they showed only a weak or no activities to leafhopper. The enzymes in the oral regurgitate secreted by the tea pests was the elicitor inducing the release of specific volatile synomone from tea shoots. These synomones possessed the systemic activity of translocation in tea plant. Reference | Related Articles | Metrics Cited: Baidu(35) Select The Composition and Chemiluminescence Analysis of Theaflavins in Enzymatic Oxidation Products of Tea Polyphenols DING Zhao-tang, WANG Xiu-feng, YU Hai-ning, SHEN Sheng-rong Journal of Tea Science    2005, 25 (1): 49-55.   DOI: 10.13305/j.cnki.jts.2005.01.009 Abstract （314）      PDF（pc） （315KB）（157）       Knowledge map    Save In this paper, theaflavins extracts from black tea and tea polyphenols were used as control, composition of theaflavins in enzymatic oxidation products was analyzed by HPLC, and the antioxidant activity was investigated by the technique of chemiluminescence. Results showed that, TF2A was the main composition in enzymatic oxidation products. In theaflavins extracts from black tea, there mainly existed four kinds of theaflavins and their contents were not greatly fluctuated. By employing CuSO4-Vc-H2O2-yeast and Fe2+-H2O2-yeast systems, antioxidant activity of enzymatic oxidation products was better than theaflavins extracts from black tea and tea polyphenols. Reference | Related Articles | Metrics Cited: Baidu(33) Select Productivity and Quality Response of Tea to Balanced Nutrient Management Including K and Mg RUAN Jian-yun, WU Xun Journal of Tea Science    2003, 23 (s1): 21-26.   DOI: 10.13305/j.cnki.jts.2003.s1.005 Abstract （368）      PDF（pc） （294KB）（402）       Knowledge map    Save This paper summarized results of experiments with potassium and magnesium fertilization on different tea types, carried out in various tea growing areas of China. Analysis of typical soil samples from various tea growing regions indicated that the supplying capacities of K and Mg in more than half samples were classified as deficient and did not meet the demands of tea plant under present production levels. Fertilization with K and Mg greatly increased yields of the major tea types, e.g. green tea, black tea, and Oolong tea. In addition to improved productivity, it is shown that quality, e.g. free amino acids, polyphenols, caffeine as well as theaflavin and thearubigin contents of black tea were largely increased. Resistances to drought and diseases were also improved by K application. Field experiments showed that potassium chloride had similar effect as potassium sulphate. The overall results confirmed that balanced nutrition including the nutrients K and Mg is an important measure to improve productivity of tea. A preliminary recommendation of K and Mg fertilization rates based on soil K and Mg status was proposed. Reference | Related Articles | Metrics Cited: Baidu(32) Select Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities KOU Xiao-hong, JIANG He-yuan, CUI Hong-chun, ZHANG Jian-yong, GAO Qing-qing, YUAN Xin-yue, SHU Ai-min, LIU Xiao-hui, GAO Qi Journal of Tea Science    2008, 28 (3): 172-180.   DOI: 10.13305/j.cnki.jts.2008.03.011 Abstract （300）      PDF（pc） （341KB）（252）       Knowledge map    Save Water-soluble green tea polysaccharides were separated and purified by Ultrafiltration membranes with pore diameter of 150 kD, 20 kD, 6 kD in turn. All the three fractions were further separated and purified by DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 column chromatography, and more than 20 green tea polysaccharide components totally were obtained. Their purity and molecular weight distribution were determined with high performance gel permeation chromatography (HPGPC) equipped with ELSD detector, and five components of homogeneous polysaccharide were obtained. The immunological activities of these green tea polysaccharides were determined by stimulated phagocytosis and nitric oxide production on RAW264.7 macrophage cell line of mice. Results showed that the tea Polysaccharides with 20 kD MW possessed significant immunological activities on NO production or phagocytosis of mice macrophage Raw 264.7 cells. Reference | Related Articles | Metrics Cited: Baidu(32) Select Study on the Change of Aroma Constituents During Pu-erh Tea Process LU Hai-peng, ZHONG Qiu-sheng, WANG Li, LIN Zhi Journal of Tea Science    2009, 29 (2): 95-101.   DOI: 10.13305/j.cnki.jts.2009.2.003 Abstract （320）      PDF（pc） （254KB）（270）       Knowledge map    Save The change of aroma constituents in Pu-erh raw tea during the pile-fermentation process were determined by HS-SPME/GC-MS. Results showed that the alcohols and hydrocarbons decreased distinctively, however, the heterocyclic oxygen compounds and esters increased distinctively during the pile-fermentation process. 1,2,3-Trimethoxybenzene was the most abundant component among the heterocyclic oxygen compounds, and its content reached the highest when the pile-fermentation process finished, and some main aromatic components, and relative contents which were obvious differences in Pu-erh raw tea and Pu-erh tea were analyzed. Reference | Related Articles | Metrics Cited: Baidu(32) Select Study on the Expression of Caffeine Synthase Gene mRNA in Tea Plant LI Yuan-hua, JIANG Chang-jun, WAN Xiao-chun Journal of Tea Science    2004, 24 (1): 23-28.   DOI: 10.13305/j.cnki.jts.2004.01.004 Abstract （291）      PDF（pc） （434KB）（175）       Knowledge map    Save The expression of caffeine synthase gene mRNA in tea plant by means of in situ hybridization was investigated. Results showed that the expressional sites is mainly located in the cell cytoplasm and nucleus of palisade tissue, but not in vacuole. The expressional sites of leaf vein are mainly in parenchyma and phloem, however, that in phloem is weaker. The expressional sites in young stalk are in phloem. The expressional sites in different cultivars are basically similar, but the expressional signal is different. Results also showed that the expressional signal of the first fresh leaf in spring is stronger than that in autumn. In terms of processing technique, the green teas which are spread 0.5 h、1 h、2 h showed apparent expressional signal, but the signal was not observed in the black teas withered for 6 h、8 h、10 h and Oolong teas shaken for 6 h、8 h、10 h. Reference | Related Articles | Metrics Cited: Baidu(31) Select Investigation and Comparison of the Aroma Components in Guangdong Black Tea WANG Qiu-shuang, CHEN Dong, XU Yong-quan, YIN Jun-feng Journal of Tea Science    2012, 32 (1): 9-16.   DOI: 10.13305/j.cnki.jts.2012.01.002 Abstract （459）      PDF（pc） （911KB）（138）       Knowledge map    Save For the purpose of studying the volatile aroma components in Guangdong black tea and determine the main aroma components, the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds in black tea samples and their contribution to the total aroma. Results showed that more than 50 aroma compounds were identified including alcohol, ester, aldehyde, ketone, terpene, hydrocarbon etc., among which the alcohol was the most important compounds and ester and aldehyde were next to flower-like, fruit-like, refreshing and sweet aroma were the main character of Guangdong black tea. Linalool, gerariol, methyl salicylate, linalool oxide and nonanal were the five most important compounds, representing 37.55%, 11.90%, 8.89%, 5.4% and 5.21% of the total aroma respectively. The alcohol was the most important compounds in Yingde tea population, the contents of aldehyde and ketone were more in Baimao tea population. Dancong and Fujian tea plant contained more terpene compounds. Reference | Related Articles | Metrics Cited: Baidu(31) Select The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme FU Dong-he, LIU Zhong-hua, HUANG Jian-an, CHEN Hui-heng Journal of Tea Science    2008, 28 (1): 62-66.   DOI: 10.13305/j.cnki.jts.2008.01.003 Abstract （284）      PDF（pc） （340KB）（169）       Knowledge map    Save Fuzhuan Tea was extracted by chloroform, ethyl acetate, 1-butanol in turn. The effect of Fuzhuan Tea on the activities of metabolize enzyme was investigated. The results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity. The sample extracted by chloroform could restrain pancreatic lipase a little and the relative activity was 0.82. The water extracts, the sample extracted by ethyl acetate and 1-butanol showed obvious activation, that of the sample extracted by 1-butanol was the most active. The multiple was 2.54. All fractions of Fuzhuan Tea were active to α-amylase, among them, the sample extracted by ethyl acetate was the most active. The relative activiy was 4.09. The water extracts was the second one and its relative activity was 2.99. That of the layer of water sample was not obvious. The sample extracted by chloroform could restrain α-amylase a little and the relative activity was 0.88. The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to α-amylase and pancreatic lipase respectively. Reference | Related Articles | Metrics Cited: Baidu(31) Select Molecular Cloning and Sequence Analysis on cDNA of Cystatin Gene from Tea Leaves WANG Zhao-xia, LI Ye-yun, JIANG Chang-jun, YU You-ben Journal of Tea Science    2005, 25 (3): 177-182.   DOI: 10.13305/j.cnki.jts.2005.03.004 Abstract （352）      PDF（pc） （232KB）（123）       Knowledge map    Save Two degenerate primers were designed according to the conserved region among the known plant cystatins. A cDNA fragment of 204βbp was amplified by RT-PCR (reverse transcription polymerase chain reaction) of total RNA extracted from fresh leaves of Tea plant (Camellia sinensis cv Longjing43). A full-length cDNA of the cystatin gene was obtained by 3’/5’RACE (rapid amplification of cDNA ends). The cDNA sequence of this 627βbp clone contained an open reading frame encoding a polypeptide of 101 amino acid residues with a predicable molecular mass of 11.026βKDa. The deduced amino acid sequence contained the motif QXVXG conserved among most members of the cystatin superfamily. By using the program of Blast on GenBank database, the sequence presented a high match with the cystatin genes from other plants, such as European chestnut, Cassava, Cowpea, Tomato, Soybean et al. All researched out sequences were all cystatins, so we can conclude that the cloned sequence is a member of cystatin gene from Tea plant. Reference | Related Articles | Metrics Cited: Baidu(31) Select A Comparative Study on Bioactivities of Tea Polyphenols and Chlorogenic Acid ZHANG Xing-hai, WANG Yue-fei Journal of Tea Science    2007, 27 (1): 39-44.   DOI: 10.13305/j.cnki.jts.2007.01.006 Abstract （418）      PDF（pc） （544KB）（186）       Knowledge map    Save Objectiveto study the differences of bioactivities of tea polyphenols (TPs) and chlorogenic acid (CHA), methods of POV assay、spectrophotometric determination and MIC were used to compare the antioxidation、scaventing free radicals and antibacterial action of TPs & CHA. Resultsin the six weeks showed that TPs (100βµg/g、200βµg/g) could restrain the POV of lard below 10βmeq/kg, antioxidation rises with concentrations of TPs, as resemble the effect of CHA (81%). On the free radicals, CHA showed selective scaventing ability, IC50(DPPH、·OH) of CHA (81%) is 65βmg/L and 135βmg/L, and CHA (20%) is 327βmg/L and 1β432βmg/L, but TPs is 52βmg/L and 1908βmg/L; In the antibacterial action, TPs and CHA can obviously inhibit both staphylococcus aureus and escherichia coli., The MIC of CHA (81%) is twice as that of CHA (20%) on staphylococcus aureus, 500βμg/g and 1β000βμg/g respectively, that of TPs is 250βμg/g, on escherichia coli.. Conclusion:TPs (95%) exceeds CHA (81%) and CHA (20%) in the antioxidation、scaventing DPPH and antibacterial activity on staphylococcus aureus, but the reverse was in the scaventing ·OH. It was regarded that TPs may play a more important role than CHA in the food and drug. Reference | Related Articles | Metrics Cited: Baidu(30) Select Study on the Gallic Acid in Pu-erh Tea LU Hai-peng, LIN Zhi, GU Ji-ping, GUO Li, TAN Jun-feng Journal of Tea Science    2007, 27 (2): 104-110.   DOI: 10.13305/j.cnki.jts.2007.02.002 Abstract （512）      PDF（pc） （484KB）（336）       Knowledge map    Save Gallic acid is a characteristic phenolic compound in Pu-erh tea with notable bioactivity. The content of gallic acid in Pu-erh tea, the change of gallic acid during the pile-fermentation, and the relation between extract fraction bioactivity and its gallic acid content were studied. Results showed that the average content of gallic acid in Pu-erh tea was 9.01βmg/g, but striking dissimilarity existed between the different samples; The gallic acid content first increased to some extent then decreased during the pile-fermentation of the solar green tea of [Camellia sinensis (Linn.) var. assamica (Masters) Kitamura]. It was found that the extracted fractions which rich in gallic acid showed higher antioxidative activities by DPPH radical scavenging assay. Reference | Related Articles | Metrics Cited: Baidu(29) Select Analysis of Differential Expression Genes in Cold-induced Tea Plant ZOU Zhong-wei, FANG Wan-ping, ZHANG Ding, DUAN Yun-shang, LI Xing-hui Journal of Tea Science    2008, 28 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2008.04.011 Abstract （271）      PDF（pc） （515KB）（149）       Knowledge map    Save The differential expression was investigated between cold-induced and normal environment in tea plant (Camellia sinensis) with cDNA-AFLP (cDNA Amplified Fragments Length Polymorphism). Expression profiles were generated around 86 special cDNA fragments (16 pairs primer, 256 primer combinations). Ten differentially expressed transcript-derived fragments were isolated. Cha1 revealed similarities (77%) to one kind of low temperature and salt responsive protein in Arabidopsis thaliana. Cha5 revealed similarities (89%) to Arabidopsis thaliana 60 S ribosomal protein L7 (RPL7B), ChaH8 had high similarities (79%) to Nicotiana tabacum A10 mRNA for stress-inducible H1 histone-like protein. Cha9 had high similarities to Populus EST from severe drought-stressed opposite wood. Other 6 fragments showed no homology to the known sequences in GenBank, perhaps they were new genes. Reference | Related Articles | Metrics Cited: Baidu(29) Select Studies on Lipid-depressing Function of Water Insoluble Tea Protein HUO Po, HUANG Guang-rong, ZHANG Xiao- hui, XIA Yong, JIANG Jia-xin, FU Jian-yun, ZHANG Ke-chang Journal of Tea Science    2005, 25 (2): 95-99.   DOI: 10.13305/j.cnki.jts.2005.02.003 Abstract （312）      PDF（pc） （211KB）（178）       Knowledge map    Save Alkali extraction technology of water insoluble protein from tea was studied. The extracting rate of tea protein was around 61.1%. The crude tea protein was prepared by adjusting pH of the extract and its purity was 54.45%.The effects of water insoluble tea protein on decreasing blood lipids were studied on the hyperlipidemia rats. Results showed that the protein had significant effects on decreasing TC、TG、LDL-C、AI and R-CHR in the hyperlipidemia rats(p<0.05). It could also modulate the HDL-C indexes. The results confirmed that the water insoluble tea protein had remarkable effects on decreasing the blood lipid of hyperlipidemia and possibly is preventing atherosclerosis and coronary heart disease. Reference | Related Articles | Metrics Cited: Baidu(29) Select Study on the Status of Lead Content in Tea Gardens of Zhejiang Province SHI Yuan-zhi, MA Li-feng, HAN Wen-yan, RUAN Jian-yun Journal of Tea Science    2003, 23 (2): 163-166.   DOI: 10.13305/j.cnki.jts.2003.02.015 Abstract （267）      PDF（pc） （292KB）（134）       Knowledge map    Save The Lead content in tea leaves from main tea producing areas of Zhejiang Province and its affecting factors have been investigated. The results showed that the air deposit was one of the main sources for lead in tea leaves coming from. It could decrease the lead content to a certain extent when fresh leaves were washed. The lead contents in tea leaves had a significantly positive relationship with the available lead contents in the soil, therefore, reducing the available lead content in the soil would be one of the effective ways to reduce the lead content in tea leaves. Reference | Related Articles | Metrics Cited: Baidu(28) Select The Development and Tendency on the New Technology Application in Tea Quality Evaluation ZHOU Yi-bin, WANG Jun Journal of Tea Science    2004, 24 (2): 82-85.   DOI: 10.13305/j.cnki.jts.2004.02.002 Abstract （362）      PDF（pc） （362KB）（203）       Knowledge map    Save Basically, the quality of tea is evaluated by the tea experts through senses. The sensitivity of human’s sense, however, is liable to be changed by external factors, therefore, it is not easy to make an accurate evaluation through human’s sense. The paper presents the new research development in evaluating tea quality such as the shape of tea, the color of tea infusion, the aroma and taste of tea by instruments, especially the application of the machine vision to the shape of tea and the color of tea infusion, the electronic nose to the tea aroma and the electronic tongue to the taste of tea, it put forwards the tendency of the new technology application to tea quality evaluation. Reference | Related Articles | Metrics Cited: Baidu(28) Select Analysis of Anatomical Structure on Tea Leaves in Shandong Province FANG Yong, MENG Zhen-nong, LI Xiu-fen, QIAO Yong-jin, MEI Li, LIANG Yu Journal of Tea Science    2004, 24 (3): 190-196.   DOI: 10.13305/j.cnki.jts.2004.03.008 Abstract （448）      PDF（pc） （44KB）（211）       Knowledge map    Save The significance and present situation on the morphology and anatomical structure of tea leaves were described in this paper. The anatomic structure of 51 tea cultivars and breeding materials were compared and the cold resistance between different cultivars and various regions were analyzed to provide theoretical basis for introduction of tea cultivar and the selection in cold resistance in northern tae area. Reference | Related Articles | Metrics Cited: Baidu(28) Select Effect of Processing on the Pb and Cu Pollution of Tea HAN Wen-yan, LIANG Yue-rong, YANG Ya-jun,SHI Yuan-zhi, MA Li-feng, RUAN Jian-yun Journal of Tea Science    2006, 26 (2): 96-101.   DOI: 10.13305/j.cnki.jts.2006.02.004 Abstract （342）      PDF（pc） （409KB）（176）       Knowledge map    Save The effect of processing on the Pb and Cu pollution of tea was studied in this paper. Results showed that tea processing was one of the main pollution sources of Pb and Cu. The extent of pollution was varied with the working procedure, machine and its metal composing. Almost every working procedure, including fresh leaves spreading out or withering, deenzyming, rolling or cutting, fermentation and drying could increase the Pb and Cu concentrations of tea. However, rolling and spreading out were the most important procedures resulting in Cu and Pb pollution, respectively. With the increase of rolling pressure and time, the Cu and Pb concentrations steadily increased. Due to different procedures, especially the rolling pressure and time, the Cu and Pb concentrations of made teas were different, oven dried and roast green tea were the highest, followed by CTC (Crushing, Tearing, Curling) and Orthodox black tea, almost no change in Longjing tea processing. The pollution sources were quite different between Cu and Pb. Cu pollution was mainly from rolling machine and the Cu content of the machine. Cu pollution from rolling procedure accounted for 90% of total one. Pb pollution was mainly due to dust, which came from unclean ground and air in the workshop. Therefore, keeping clean in workshop and regulating the metal constitution of rolling machine could reduce the Pb and Cu pollution during tea processing. In addition, clearing the mature leaves and tea dust away after tea primary processing also could decrease the Pb concentration of tea. Reference | Related Articles | Metrics Cited: Baidu(28) Select Study on Clarification of Oolong Tea Beverage by Ultra-filtration YIN Jun-feng, QUAN Qi-ai, LUO Long-xin, QIAN Xiao-jun Journal of Tea Science    2003, 23 (s1): 58-62.   DOI: 10.13305/j.cnki.jts.2003.s1.011 Abstract （330）      PDF（pc） （310KB）（140）       Knowledge map    Save The Oolong tea juice was ultra-filtrated by using the plate-frame module and choosing three kinds of CA membrane with three pore diameter . Study on effect of different ultra-filtration process on the contents of main compounds including tea polyphenols, amino acids, caffeine, soluble sugar, pectin, protein etc in Oolong tea juices and colour difference, sensory quality, pH, ultra-filtration flux, etc. The results showed that ultra-filtration can clarify the Oolong tea juice effectively. The ultra-filtration can obstruct high-molecular mass that easy to participate higher retaining rate of the main compounds under the suitable ultra-filtration parameter. Sensory quality of the green tea juice ultra-filtrated was fine. Reference | Related Articles | Metrics Cited: Baidu(27) Select Effects of Acidification and Organic Matter Accumulation on Lead Bio-availability in Tea Garden Soils ZHANG Ming-kui, FANG Li-ping, ZHANG Lu-qing Journal of Tea Science    2005, 25 (3): 159-164.   DOI: 10.13305/j.cnki.jts.2005.03.001 Abstract （303）      PDF（pc） （73KB）（138）       Knowledge map    Save The increased accumulation of lead (Pb) in tea is widely concerned. The study on the factors influencing Pb concentration in tea is necessary for the production of high quality tea. In this study, soils of two tea gardens (Yellowish red soil and Red soil) were collected and incubated by adding acid and organic manure for studying the effects of acidification and organic matter accumulation on Pb bio-availability of tea garden soils. Water-soluble Pb, NH4OAc extractable Pb and chemical forms of Pb in the incubated soils were characterized. The results showed that soil acidification promoted transformation of other forms of Pb into exchangeable Pb, and thus increased water solubility and bio-availability of Pb in the soils. Accumulation of organic matter could activate Pb in soils with pH>4, whereas accumulation of organic matter decreased Pb bio-availability in lower pH soils (pH<4). The effects of pH and organic matter on Pb bio-availability were more significant in sandy soil than that in clayey soil. Reference | Related Articles | Metrics Cited: Baidu(27) Select Combination of Particle Bombardment-mediated and Agrobacterium-mediated Transformation Methods in Tea Plant WU Shan, LIANG Yue-rong, LU Jian-liang, LI Hao-yan Journal of Tea Science    2005, 25 (4): 255-264.   DOI: 10.13305/j.cnki.jts.2005.04.004 Abstract （397）      PDF（pc） （376KB）（106）       Knowledge map    Save A microprojection protocol using particle delivery system and GUS report gene was developed for tea plant (Camellia sinensis) callus. Comparison of several pre-bombardment culture media showed the highest transient gene expression in the media with PVP. Instead of making contribution to improving GUS expression, osmotic pre-treatment would cause the target tissue having difficulty in regeneration. High transformation rate was obtained using 125 μg tungsten coated with 0.25 μg DNA per shot. Bombarding tea callus at 7 MPa with a target distance of 5 cm and one time was suitable for both GUS activity and regeneration. Several transformation methods, including Agrobacterium-mediated transformation(AGR), particle bombardment (BOM), and combination of the two methods (BTA, BPA and BOA), have been tested and compared with each other. Based on the results of GUS expression and survival rates of the resistant calli, the method combining Agrobacterium and particle bombardment systems is suitable to apply for tea plant transfortion. Reference | Related Articles | Metrics Cited: Baidu(26) Select Study on the Key Technology and Equipment for Low Caffeine Tea Processing SUN Cheng, QUAN Qi-ai, JIN Shou-zhen Journal of Tea Science    2003, 23 (s1): 68-72.   DOI: 10.13305/j.cnki.jts.2003.s1.013 Abstract （474）      PDF（pc） （152KB）（187）       Knowledge map    Save The technology of de-enzyming of tea leaf by hot water for the purpose of minimizing the caffeine in tea was discussed. Be combined with dehydration, rolling and drying, the tea had the style of the traditional roasting green tea and the caffeine contents were reduced as high as 67%. The major constituents could be as high as 82%. This leaf dredging up machine is characterized by the advantage of simple structure, convenient handing, high rate of taking off caffeine, and saving water and energy. Reference | Related Articles | Metrics Cited: Baidu(26) Select Influence of Different Enzyme Origin on the Enzyme-Catalysing Synthesizing of Theaflavins in Single and Bi-liquid Phases Systems GU Ji-ping, LIU Zhong-hua, HUANG Jian-an, SHI Zhao-peng, WANG Gui-xue Journal of Tea Science    2007, 27 (1): 76-82.   DOI: 10.13305/j.cnki.jts.2007.01.012 Abstract （339）      PDF（pc） （300KB）（207）       Knowledge map    Save Synthesizing theaflavins (TFs) in single and bi-liquid phase systems was studied by various source of PPOs. The results showed that different effects could be gained in synthesizing the theaflavins by using the various source of PPOs. As to PPO from fresh tea leaves, the effect in bi-liquid phase system was more remarkable than that in single liquid phase system. The content of TFs in ethyl acetate layer in single liquid phase system, amounted at 33.74%, the synthesizing rate of TFs was 14.245%, while in bi-liquid phases system tests, the content of TFs in ethyl acetate layer was 39.74%, and the synthesizing rate of TFs was 31.792%. When the PPO was from pear, the effect in bi-liquid phase was slightly better than that in single liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 45.73%, the synthesizing rate of TFs was 18.799%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 40.20%, and the synthesizing rate of TFs was 22.11%. However, when the PPO from apple, opposite results was gained that the effect in single liquid phase was better than that in bi-liquid phase, the content of TFs in ethyl acetate layer, in single liquid phase system, amounted at 18.22%, the synthesizing rate of TFs was 13.34%, while in bi-liquid phases system, the content of TFs in ethyl acetate layer was 11.56%, and the synthesizing rate of TFs was 6.935%. Reference | Related Articles | Metrics Cited: Baidu(26) Select Study on Separation and Concentration of Tea Polysaccharides by Membrane System YIN Jun-feng, YUAN Hai-bo, GU Ji-ping, LIN Zhi-zhong, CHEN Jian-xin, TAN Jun-feng, WANG Fang Journal of Tea Science    2006, 26 (2): 108-111.   DOI: 10.13305/j.cnki.jts.2006.02.006 Abstract （318）      PDF（pc） （365KB）（169）       Knowledge map    Save The 50βK membrane has been ascertained as the best membrane for separation and concentration of tea polysaccharides, and on the basis of the result, the treatment had been optimized, and the result showed that contents of total sugar reached 30.37%, and those of tea polysaccharides arrived at 16.89%. In compared with the control, the scavenging rate of ·OH were increased by 39.89%. The experiment showed that tea polysaccharides is separated and concentrated by membrane, and the activity of tea polysaccharides could be remained. Reference | Related Articles | Metrics Cited: Baidu(26) Select Advances on DNA Molecular Markers and Gene-engineering in Tea Plants WANG Li-yuan, CHENG Hao, ZHOU Jian Journal of Tea Science    2004, 24 (1): 12-17.   DOI: 10.13305/j.cnki.jts.2004.01.002 Abstract （422）      PDF（pc） （333KB）（246）       Knowledge map    Save Advances on gene-engineering in tea plants are reviewed from following aspects: DNA molecular markers, isolation and cloning of genes, genetic transform system. The problems and probable development in future are also prospected. Reference | Related Articles | Metrics Cited: Baidu(25) Select Aluminium in Tea Soils, Rhizosphere Soil and The Characteristics of Al Uptake by Tea Plant RUAN Jian-yun, WANG Guo-qing, SHI Yuan-zhi, MA Li-feng Journal of Tea Science    2003, 23 (s1): 16-20.   DOI: 10.13305/j.cnki.jts.2003.s1.004 Abstract （325）      PDF（pc） （288KB）（202）       Knowledge map    Save This paper summarized several experiments investigating aluminium in tea soil, rhizosphere and its uptake by tea plants. The main points are as follows. 1. The content of exchangeable Al (1βmol/L KCl) ranged from 2.5 to 988βmg/kg with an average of 238βmg/kg. Amorphous and interlay Al were the main fractions. Tea planting intended to increase of water soluble and exchangeable Al fractions. 2. The content of exchangeable Al increased with the amount of applied nitrogen as urea. Exchangeable Al in rhizosphere (1 mm from the root surface) increased significantly when NH4+-N was supplied. 3. Al absorption by intact tea root was a saturation process with a fast phase at the initial stage and lagged behind after some 6 h. 4. Al below 400βµmol/L promoted the growth of root, especially increased the number and total length of lateral roots. Reference | Related Articles | Metrics Cited: Baidu(25) Select Properties of Rhizosphere Soil of Tea Plants and The Impact of Nitrogen Fertilizer RUAN Jian-yun, MA Li-feng, SHI Yuan-zhi Journal of Tea Science    2003, 23 (2): 167-170.   DOI: 10.13305/j.cnki.jts.2003.02.016 Abstract （259）      PDF（pc） （241KB）（125）       Knowledge map    Save The properties of rhizosphere soil of pot-cultured tea plants were investigated. The rhizosphere soil was collected by removing the soil adhering to plant roots and other soil was referred as bulk soil. N fertilization with NH4+ (urea or (NH4)2SO4) significantly reduced soil pH, the contents of exchangeable Ca, K, Mg and base saturation whereas raised extractable Al levels. Compared to bulk soil, rhizosphere soil showed lower pH, exchangeable Ca, Mg and base saturation but a higher exchangeable K. The contents of soil solution Al, exchangeable Al and absorbed hydroxy Al were much higher in the rhizosphere soil than those in the bulk soil. The results showed that the acidification of rhizosphere soil increased the availability of Al, which might be of importance for tea plants capable of taking up a large amount of Al. Reference | Related Articles | Metrics Cited: Baidu(25) Select Study on the Toxicity Experiments of Fuzhuan Tea XIAO Wen-jun, FU Dong-he, REN Guo-pu, GONG Zhi-hua, XIAO Li-zheng, LIU Zhong-hua Journal of Tea Science    2007, 27 (4): 307-310.   DOI: 10.13305/j.cnki.jts.2007.04.008 Abstract （372）      PDF（pc） （326KB）（197）       Knowledge map    Save The toxic experiment of Fuzhuan tea was carried out by using the extracted instant Fuzhuan tea as material in order to evaluate its safety for the mice and rat. The results showed that the LD50 of male mice and female mice was 14β700βmg/kg·bw and 19β600βmg/kg·bw respectively(the limit reliability was 10β600~36β000), and both of the Ames test and the semen teratogenicity results were negative. During the 30 days fed by the feeding mixed with 0.85%, 1.25%, 1.70% instant Fuzhuan Tea for the rat rats of each dose group grew well, the hemogram indexes of rats from each dosage, blood biochemical indexes and triglyceride, Urea anhydride, blood muscle anhydride and blood sugar indexes, ratio of spleen to body weight, kidney to body weight, and the histopathological characteristic of all test rats showed no significantly difference (P<0.05), which suggested Fuzhuan Tea was a no poisonous food. Reference | Related Articles | Metrics Cited: Baidu(24) Select Chemical Forms of Lead and Cadmium in Soils from Some Tea Gardens near Highway ZHANG Ming-kui，HUANG Chang-yong Journal of Tea Science    2004, 24 (2): 109-114.   DOI: 10.13305/j.cnki.jts.2004.02.007 Abstract （277）      PDF（pc） （358KB）（184）       Knowledge map    Save Three soil profiles and twenty-three surface soil samples, developed from various parent materials, were collected from different tea gardens near highway in Zhejiang province. The total, five chemical fractions (exchangeable, carbonate bound, organic matter-bound, oxides bound and residual forms) and water-solubility of Pb and Cd in the soils were characterized. The results showed that concentrations of total and different chemical fractions of Pb and Cd in the soils varied with locations and soil properties. Increased Pb and Cd concentrations in surface layer of the soil profiles suggested that Pb and Cd accumulation in the soils was related to soil pollution and fertilizer application. The concentrations of organic matter-bound, oxides bound and residual forms of Pb and Cd were mainly controlled by total Pb and Cd in the soils. However, exchangeable and water-soluble Pb and Cd, which had high potential risk, increased with decreasing pH. The results indicate that long-term plantation of tea could cause sol acidification and elevated concentrations of bio-available heavy metals in the soil, and hence aggravated the risk of heavy metals. Reference | Related Articles | Metrics Cited: Baidu(24) Select Adsorption Kinetics of Catechins onto Tea Stalk Lignocellulose YE Jian-hui, JIN Jin, LIANG Hui-ling, LIANG Yue-rong Journal of Tea Science    2008, 28 (5): 313-318.   DOI: 10.13305/j.cnki.jts.2008.05.004 Abstract （352）      PDF（pc） （320KB）（242）       Knowledge map    Save Lignocelluloses prepared from woody tea stalk was used to adsorb tea catechins from green tea extract solution by static adsorption, focusing on the study of adsorption isotherm equation and characteristics of kinetics. The results showed that the Freundlich isotherm equation could be used to describe the adsorption of total catechins, and the adsorption capacities of total catechins decreased with the increase in temperature. The equations of pseudo-first-order model and pseudo-second-order model were used for the simulation of adsorption kinetic study. The kinetic results were more fitted to the pseudo-second-order model than the pseudo-first-order model. The saturated adsorption capacities on catechins calculated by the pseudo-second-order model were close to those of experimental data at different temperatures. With the increase in temperature, the rate constants of k1 and k2 are increased, while the initial adsorption rate of total catechins are decreased. Reference | Related Articles | Metrics Cited: Baidu(24) Select Effects of Different Radiation Treatments on The Content of Chemical Components and Quality of Puer-Tea ZHOU Shu-hong, GONG Shu-ying Journal of Tea Science    2003, 23 (1): 51-56.   DOI: 10.13305/j.cnki.jts.2003.01.011 Abstract （305）      PDF（pc） （363KB）（199）       Knowledge map    Save Through experiment with treatments of various dosages of radiation and different initial water contents in Puer-tea, it was found that contents of amino acid, tea polyphenols and the oxidation products of polyphenols were increased; oppositely, caffeine and catechins, particularly the compound catechins were decreased; In addition, the radiation treatments had no negative effect on the organoleptic quality of Puer-tea. Reference | Related Articles | Metrics Cited: Baidu(23) Select Effects of Wet-Scenting Process and RJF on the Aroma Constituent of Jasmine Scented Tea YE Nai-xing, YANG Guang, ZHENG Nai-hui, YANG Jiang-fan, WANG Zhen-kang, LIANG Xiao-xia Journal of Tea Science    2006, 26 (1): 65-71.   DOI: 10.13305/j.cnki.jts.2006.01.011 Abstract （411）      PDF（pc） （424KB）（225）       Knowledge map    Save The aroma constituent of high-quality jasmine-scented tea was analyzed using SPME/GC-MS method. It was found that cis-3-hexenyl acetate, linalool, benzyl acetate and methyl anthranilate were the major fragment components in jasmine scented tea. The contents of cis-3-hexenyl acetate, linalool, methyl anthranilate, cadinene, indole, benzyl alcohol, nerolidol, geranyl acetate were significantly increased in the jasmine scented tea made by wet-scenting process. The content of aromatic components in jasmine scented tea was increased when the rate of jasmine flower to tea (RJF) was ranged from 69% to 115%. When RJF is above 96%, the content of major aromatic components in the jasmine scented tea did not increase significantly. It suggested that the absorbance of tea to the fragment of jasmine flower would be attained a balance in the RJF. It suggested that the suitable RJF is 90% to 100% for producing high-quality jasmine tea made by wet-scenting process. Reference | Related Articles | Metrics Cited: Baidu(23) Select Sampling Strategy to Establish a Primary Core Collection of Chinese Tea Germplasms WANG Xin-chao, LIU Zhen, YAO Ming-zhe, MA Chun-lei, CHEN Liang, YANG Ya-jun Journal of Tea Science    2009, 29 (2): 159-167.   DOI: 10.13305/j.cnki.jts.2009.2.012 Abstract （373）      PDF（pc） （266KB）（171）       Knowledge map    Save In order to rapid and precisely identify the excellent gene resource for the breeding of tea plant, the construction on core collection of tea plant is necessary. Based on the passport and evaluated datum of 1048 tea accessions in “Evaluated database of tea germplasms”, the optimum sampling proportion, grouping methods, intra-group sampling proportion and methods, in order to obtain the optimum sampling strategy to establish the core collection of China tea germplasm. Results showed that, (1) 20% was the optimum sampling proportion, at this proportion, the genetic diversity index of core collection was higher than other proportions, and the reserved ratio of phenotypic charater of core collection was higher than 95%. (2) At 20% sampling proportion, 25 primary core collections using 3 grouping methods, 4 intra-group sampling proportions, 2 intra-group individual selecting methods were evaluated using 5 parameters to determine the optimum sampling methods. The five parameters were a) ratio of phenotype reserved, b) phenotypic of variance value, c) variance of phenotypic frequency, d) Shannon-weave genetic diversity index, e) coefficient of variation quantitative characters. Results showed that there were different ranks when different evaluated parameter was used. It implied that there was significant interaction between grouping methods, sampling proportion and sampling methods within group. Combined the ranking results of 5 parameters, the optimum sampling strategy to establish primary core collection of tea plant is cultivated region grouping-logarithm sampling proportion-cluster individual selecting. Reference | Related Articles | Metrics Cited: Baidu(23) Select Estimating the Quality of Tea Leaf Materials Based on Contents of Moisture, Total Nitrogen and Crude Fiber by NIR-PLS Techniques WANG Sheng-peng, WAN Xiao-chun, LIN Mao-xian, ZHANG Zheng-zhu Journal of Tea Science    2011, 31 (1): 66-71.   DOI: 10.13305/j.cnki.jts.2011.01.011 Abstract （314）      PDF（pc） （220KB）（176）       Knowledge map    Save In order to estimate the quality of the fresh tea leaves by near infrared spectrum (NIRS) technique, the correlative model equation between near infrared spectrum of the fresh tea leaves and their corresponding moisture, crude fiber and total nitrogen amounts respectively through cross-validation and partial least squares (PLS) method were established in this paper. When the dimension was 7, the three indicators of R value were 0.92, 0.86 and 0.90, root mean square error of cross-validation (RMSECV) were 0.769, 0.332 and 0.742, respectively. A quality coefficient equation was suggested depending on the contents of moisture, crude fiber and entire nitrogen of fresh tea leaves. According to the quality coefficient equation of fresh tea leaf raw material, the quality coefficient of fresh tea leaves was obtained. The larger quality coefficient is, the higher quality of fresh leaf is. Thus, we developed a new method for the evaluation for the quality of fresh tea leaves by using NIRS was developed. Reference | Related Articles | Metrics Cited: Baidu(22) Select Effect of Al and F Interaction on the Tea Quality WANG Xiao-ping, LIU Peng, LUO Hong, XIE Zhong-lei, XU Gen-di, CHEN Li-jun Journal of Tea Science    2009, 29 (1): 9-14.   DOI: 10.13305/j.cnki.jts.2009.1.002 Abstract （281）      PDF（pc） （177KB）（159）       Knowledge map    Save In this research, quality of tea leaves to aluminum (Al) and fluorine (F) interaction were studied, and five indices (polyphenols, caffeine, free amino acids, vitamin C and chlorophyll concentrations) in tea leaves (variety Anji Baicha and zhirenzaocha) were investigated after 30 d in hydroponic culture. The nutrient solution was treated by the interaction of Al (0, 30, 90 mg/L) and F (0, 4, 12 mg/L). The results were summarized as follows: With 30 mg/L of the single Al, the contents of polyphenols, caffine, free amino acids, vitamin C and chlorophyll in tea leaves were increased. But when the concentration of Al was 90 mg/L, the contents of above components in tea leaves were decreased. These components in tea leaves were increased under the treatment of 4 mg/L of the single F and decreased with the treatment of 12 mg/L of the single F. In the process of aluminum and fluorine interaction, the contents of these components in tea leaves of Anji Baicha were increased during the treatments of Al/F ratioin 30/4, 30/12, 90/4 and 90/12. However, zhirenzaocha were decreased. The results showed that tea quality improved in low concentration of aluminum or fluorine. Meanwhile, the variance effect of interaction was correlated to different proportion of Al/F and the proportion of Al/F for better quality of Anji Baicha and zhirenzaocha was different indicating there existed a varietal difference. Reference | Related Articles | Metrics Cited: Baidu(22) Select Influence of Microwave-assisted Extraction on the Composition and Yield Rate of Catechins in Green Tea LI Min, REN Guo-pu, CUI Li, XIAO Li-zheng, XIAO Wen-jun Journal of Tea Science    2011, 31 (2): 112-118.   DOI: 10.13305/j.cnki.jts.2011.02.006 Abstract （278）      PDF（pc） （314KB）（144）       Knowledge map    Save Microwave-assisted extraction of green tea showed certain effect on catechins yield rate and composition of catechins, however, it lack of systematic research. Based on systemic investigation on the influence of extracting power, extracting time, ratio of tea and water, water temperature and extracting times of microwave-assisted extraction on the composition and yield rate, the test processing parameters with the least influence of microwave extraction and higher yield rate on the composition were optimized and screened, which provide the reference for application in the industrialization of microwave-assisted extraction on tea by using the green tea as the raw material and the composition and yield rate in the extraction as the major index and used the industrial water as the control and the orthogonal test as the method of verification. Under the above mentioned parameters, the microwave-assisted extraction of green tea showed the least influence. The results showed that the optimum parameters were obtained at the ratio of solid to liquid of 1﹕25, microwave-assisted extracting power of 539W, microwave extracting time of 2min, extracting water temperature of 90℃, and the two times of extracting. The rate of EGC﹕DL-C﹕EC﹕EGCG﹕GCG﹕ECG was 3.25﹕0.12﹕1.00﹕7.66﹕0.49﹕1.82,the yield rate of the individual catechins was 130.47%、22.98%、124.62%、114.40%、7.39%、96.64% of yield rate extracted by industrial water. Compared with the current industrial technology, this method showed better practicability and results for the extraction of tea polyphenol and catechins. Reference | Related Articles | Metrics Cited: Baidu(22) Select Changes of Aroma Constituents during Zuoqing Procedure and Its Relation to Oolong Tea Quality HUANG Fu-ping, CHEN Rong-bin, LIANG Yue-rong, CHEN Wei, LU Jiang-lian, CHEN Chang-song, YOU Xiao-mei Journal of Tea Science    2003, 23 (1): 31-37.   DOI: 10.13305/j.cnki.jts.2003.01.007 Abstract （385）      PDF（pc） （353KB）（168）       Knowledge map    Save Changes of aroma constituents during Zuoqing procedure and their relation to Oolong tea quality were studied by GC/MS analysis by using the shoots of Camellia sinensis Var. Wuyi Rougui. The results indicated that the concentration of essential constituents such as hexanal, (E)-2-hexenal 1-pentanol, linalool oxide(I), linalool oxide(II), (z)-hexanoic,3-hexenyl ester+benzeneacetaldehyde, .α-farnesene, geraniol, phenylethyl alcohol, β-ionone, jasmone, nerolidol, indole increased with the increasing of the intensity of Zuoqing treatment, which resulted in the improvement of Oolong tea quality. It was also shown that nerolidol was the characteristic aroma consituent of Fujian Oolong tea and it can be used as one of indicators of the quality of Rougui Oolong tea. Reference | Related Articles | Metrics Cited: Baidu(22) Select Modeling for the Optimization of Fertilization of Tea High Yield XIA Jian-guo, LI Jing, GONG Fa-yong, WU De-yong Journal of Tea Science    2005, 25 (3): 165-171.   DOI: 10.13305/j.cnki.jts.2005.03.002 Abstract （318）      PDF（pc） （431KB）（140）       Knowledge map    Save The return two times set up juices optimumly trying to adopt“311-B”,a three-factor (N,P,K)substrate cultivation experiment was conducted by using tea. The regression equations of the relationship between N, P, K and the effects on the yield were established. The results indicated that the yield of tea would increase with high nitrogen supply, and P, K had certain effects on it, the order is N>P>K, the interactions of N-P, N-K and P-K to the yield were significant. Considering both the yield and quality of the hot pepper, the combinations of N, R K were optimized as follows: N: 0.406~0.435βg/kg soil, P2O5: 0.159~0.170βg/kg soil, K2O: 0.186~0.198βg/kg soil, and N:P2O5:K2O was 1:0.39:0.46. Reference | Related Articles | Metrics Cited: Baidu(22) Select Attracting Effect of Volatile Infochemicals from Tea Shoots and Flowers on Winged Tea Aphids HAN Bao-yu, ZHOU Cheng-song Journal of Tea Science    2004, 24 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2004.04.005 Abstract （295）      PDF（pc） （47KB）（153）       Knowledge map    Save The attracting effects of the fifteen volatile components emitted from tea shoots and tea flowers on alate tea aphid, Toxoptera aurantii, were measured by four-arm olfactometer and electroantennogram (EAG). The result showed that in those odors (Z)-3-hexen-1-ol, (E)-2-hexenal, methyl salicylate, linalool, n-octanol and hexanal as well nerol and allyl isothiocyanate, the main aroma components from tea flowers showed significant attracting effect on the winged tea aphids. The difference in aphid number attracted by 2 doses of 10-4βg/ml and 10-2βg/ml of 15 components was obvious in olfactometer bioassay. EAG responses showed that: ① the EAG value by the odor from the fresh 1st leaf below the bud was largest; ② Simulating the “green leaf volatiles”, a mixture composed of 10-2βg/ml n-hexanol, n-pentanol, 1-penten-3-ol, 2-penten-1-ol, (E)-2-hexenal, (Z)-3-hexen-1-ol and (Z)-3-hexenyl acetate according to ratio of 1∶1∶1∶1∶1∶1∶1, could elicit strong EAG responses; ③ the odor from aphid-damaged tender stem repelled tea aphids. It was supposed that the green leaf volatiles and methyl salicylate liberated from tea shoots and tea flowers play a role of attracting lures in alate tea aphids foraging and orienting to tea shoots, and the aphid-damaged tea shoots maybe emit infochemicals to dispel tea aphids. Reference | Related Articles | Metrics Cited: Baidu(22) Select Molecular Cloning and Expression Analysis on ACC Oxidase Gene Full-length cDNA from Tea Plant ZHANG Ya-li, QIAO Xiao-yan, CHEN Liang Journal of Tea Science    2008, 28 (6): 459-467.   DOI: 10.13305/j.cnki.jts.2008.06.006 Abstract （334）      PDF（pc） （527KB）（83）       Knowledge map    Save 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase is an important enzyme and plays a critical regulatory role in the biosynthesis of ethylene. Based on previous ESTs sequencing results of the tender shoots cDNA library and RT-PCR technology, a full-length cDNA sequence coding ACC oxidase (ACO) of tea plant was cloned. The GenBank accession is DQ904328 in the NCBI. The ACO gene had 1 232 bp in length, encoding 320 amino acid residues with the putative molecular weight of 36.2 KD and the pI 5.41. The protein sequences of tea plant ACO aligned with those of other 12 plants showed highly conservative sequences in tea plant. Neighbor-Joining phylogenetic tree based on ACO sequence of tea plant and other 22 plants indicated that tea plant had very close relationship with that of Diospyros kaki. The ACO gene expression level of different cultivars after high and low temperature stresses were analyzed using RT-PCR method. The expression level has correlation with resistance of the cultivars to certain degree. Reference | Related Articles | Metrics Cited: Baidu(22) Select Characteristics of Soil Nutrients and Enzyme Activity of Ancient Tea Garden in Jingmai, Lancang, Yunnan Province JIANG Hong, SHA Li-qing Journal of Tea Science    2008, 28 (3): 214-220.   DOI: 10.13305/j.cnki.jts.2008.03.008 Abstract （286）      PDF（pc） （275KB）（182）       Knowledge map    Save Ancient tea gardens in Yunnan Province are regarded as the provenance of Pu-erh Tea, and the ancient tea garden (ATG) in Jingmai, Lancang County is a typical example of cultivated ATGs. In order to investigate soil nutrients and enzyme activity, the ATG soil in Jingmai and its neighboring conventional tea garden (CTG) and forest soils were sampled. Soil pH, organic matter (OM), total N (TN), total P (TP), total K (TK), alkaline hydrolyzed N (AN), available P (AP), available K (AK), Ca, Al, Mg, activity of acid phosphatase, catalase, urease and saccharase were analyzed. The results showed that (1) All the soils were acid and at a pH of 3.80~3.91, and pH in tea garden soils was lower than in forest soil. (2), The OM, N, P and soil enzyme activity were rich in top soil (0~20 cm) and poor under 20 cm in different soil layers, while K, Mg and Al showed no obvious difference in different layers. (3) OM was rich in all soils, and the contents of OM, TN and AN were CTG < ATG < forest, while TP and AP were richest in ATG. (4) Enzyme activity showed different distribution patterns in the three soils. (5) Soil pH correlated with metal elements, N existed the most significant correlation with other nutrients, and soil nutrients did certain correlation with soil enzymes, especially N, C, P, K. Reference | Related Articles | Metrics Cited: Baidu(22) Select Selection of Optimum Conditions for Theaflavin Transformation from Tea Polyphenol through Immobilized Enzyme Membrane TU You-ying, FANG Qing, LIANG Hui-ling, HUANG Hai-tao Journal of Tea Science    2004, 24 (2): 129-134.   DOI: 10.13305/j.cnki.jts.2004.02.011 Abstract （272）      PDF（pc） （393KB）（97）       Knowledge map    Save Immobilized polyphenol oxidases (IPPO) was applied for the synthesis of theaflavins using tea catechins as substrates. A Response Surface Methodological (RSM) study was conducted using a five-level, five-variable, central composite rotation design (CCRD) to select the optimum catalysis conditions for theaflavin transformation from tea polyphenol through immobilized enzyme membrane. Five important variables were considered, namely, incubation duration, enzyme/substrate ratio (E/S, Activity Units/ml-AU/ml), aeration volume, concentration of tea polyphenols, and the pH value of the reaction system. The highest theaflavins concentration of 0.766βmg/ml was predicted under the conditions as with 49βmin for the incubation duration, 128.7 for E/S ratio, 23.81βml/min ventilation for air volume, 5.95βmg/ml for catechins concentration, and pH 4.3. The measured theaflavins concentration was 0.754±0.017βmg/ml under the same condition, which suggested that the prediction model was practical and the selected conditions were reasonable. Reference | Related Articles | Metrics Cited: Baidu(21) Select Study on the Optimum Machine-plucking Period for High Quality Tea LUO Yao-ping, TANG Meng, CAI Wei-zhi, WEN Dong-hua, WEN Zheng-jun Journal of Tea Science    2008, 28 (1): 9-13.   DOI: 10.13305/j.cnki.jts.2008.01.007 Abstract （333）      PDF（pc） （260KB）（120）       Knowledge map    Save On the basis of requirements for producing high quality tea, the seasonal variation of mechanical compositions of machine-plucked tea shoots under three plucking period was investigated when the following three criterions for tea shoots was pre-set up in one bud with one leaf and one bud with two leaves, and two-leaf banjhi. Results showed that high quality tea could be produced when the tea shoots were plucked in the period that 75.0%~84.9% shoots were met the one bud and tow leaves stage, and therefore this period can be defined as the optimum machine-plucking time. It was also showed that if machine-plucking is performed based on the above-defined criterion, the interval of machine-plucking in spring would be around 20 days, if an optimum period of machine-plucking is defined on the basis of the formation of a fish-leaf, it could be 15 days after the formation of fish-leaf in spring and 12 days in autumn. The average maturing time of a tea-leaf is 5.4 days in spring and 4.2 days in autumn in Hangzhou. Thus, a suitable regulation of machine-plucking period, will obtain the required plucked tea shoots for the manufacture of high quality tea. Reference | Related Articles | Metrics Cited: Baidu(21) Select Study on Activity of Dark Tea Extracts on PPARs Model SONG Lu-bin, HUANG Jian-an, LIU Zhong-hua, HUANG Hao, WANG Kun-bo Journal of Tea Science    2008, 28 (5): 319-325.   DOI: 10.13305/j.cnki.jts.2008.05.002 Abstract （372）      PDF（pc） （329KB）（183）       Knowledge map    Save Pu-er tea, Liu-bao tea, Fu-zhuan tea, Hua-zhuan tea, Qing-zhuan tea and Hei-zhuan tea as study material, and Tuo tea, Mi-zhuan tea as contrast material, the activity of dark tea on PPARδ and PPARγ model was studied. The results showed that the activation of dark tea to PPARδ & PPARγ receptor is high, and the activating components in dark tea on PPAR can be extracted by hot water. Therefore, dark tea showed a certain role on Hyperlipidemia, anti-obesity, regulation of carbohydrate metabolic and antiatherosclerosis. Reference | Related Articles | Metrics Cited: Baidu(21) Select Effect of Cadmium Ions on Photosynthetic Structure and Its Functions of Tea Leaves SU Jin-wei, WANG Xiang-Ping Journal of Tea Science    2004, 24 (1): 65-69.   DOI: 10.13305/j.cnki.jts.2004.01.013 Abstract （341）      PDF（pc） （726KB）（255）       Knowledge map    Save Tea plant was cultivated in Hoagland nutrient solution containing 0.5βmmol/L Cd2+ for 8 days. Tea leaves lost their green color gradually with the change in the ultrastructure of the chloroplast . At the early stage, there was a slight decrease in the number of granum thylakoids. Then, the thylakoids piled up loosely, and chloroplasts rounded off to different extents. At the late stage , the structure of the chloroplast related to thylakoid became unclear, while the thylakoid system was disintegrated abnormally with a few single bent and swell thylakoids, and some ruptured envelope of chloroplast. Cadmium ions significantly inhibited the photosynthesis activity and led to the decline in the chlorophyll content and chlorophyll a/b ratio. Further more the photochemical efficiency of PSII（Fv/Fm）and the potential activity of PSII（Fv/Fo）decreased in different degrees with increasing Cd2+ stress . The pollution duration resulted in the damage of photosynthetic structure in tea leaf cells. Reference | Related Articles | Metrics Cited: Baidu(21) Select Extracting Process of Teasaponin and the Application in Detergent LI Yun-tao, JIA Bin Journal of Tea Science    2006, 26 (3): 199-203.   DOI: 10.13305/j.cnki.jts.2006.03.009 Abstract （275）      PDF（pc） （248KB）（239）       Knowledge map    Save A teasaponin is extracted from the tea seed, and then a well-behaved washing assistant is prepared by the teasaponin blended with sodium perborate. The influencing factor in the different extract method, and the application results were investigation. Results indicates, in the experiment terms, a satisfactory result were attained, under the experimental condition, and the detergent showed a good application results. Reference | Related Articles | Metrics Cited: Baidu(21) Select Study on the Extraction of Tea Juices from Fresh Green Tea Leaves ZHANG Ling-yun, LIANG Yue-rong, SUN Qi-fu, SUN Qing-lei, LU Jian-liang Journal of Tea Science    2003, 23 (1): 46-50.   DOI: 10.13305/j.cnki.jts.2003.01.010 Abstract （308）      PDF（pc） （298KB）（168）       Knowledge map    Save An orthogonal experiment was applied to study the effect of extraction temperature, duration and ratio of tea to water on the quality of fresh green tea juice. The results showed that with rising of extracting temperature and extending of extracting duration, the extracted solids yield increased, the color of infusion turned to yellow and lightness of infusion decreased, and tea cream raised obviously. The optimum conditions of the extraction were as fallows: the ratio of tea to water 1:60, the temperature 50℃, and the extraction duration 10βmin. Reference | Related Articles | Metrics Cited: Baidu(21) Select Cloning and Expression Analysis of Stress-resistant ERF Genes from Tea Plant [Camellia sinensis (L.) O. Kuntze] CHEN Lin-bo, FANG Chao, WANG Yu, LI Ye-yun, JIANG Chang-jun, LIANG Ming-zhi Journal of Tea Science    2011, 31 (1): 53-58.   DOI: 10.13305/j.cnki.jts.2011.01.009 Abstract （331）      PDF（pc） （269KB）（136）       Knowledge map    Save For A TDF, which has been gained from genes expressed differentially in tea plant under cold stress using cDNA-AFLP, containing a complete coding sequence cDNA was cloned by RACE,named CsERF, and contains an ORF, which encodes a polypeptide of 212 amino acids including a conserved AP2 domains. Sequence alignment showed that CsERF protein shared high identity with other plants. qRT-PCR analysis showed that ERF gene was up-regulated by cold, ethylene, dehydration, NaCl and the maximum expression were 121.1, 22.6, 2.6, 2.2 times higher than before treatment, respectively. For different tea organs, transcriptional level of the ERF gene was the highest in mature leaves, the next was in bud, the third was in root and stem, and the lowest in flower and seed. It deduced that ERF gene was very important for tea plant in response to abiotic stress and was strictly controlled in expression of different organs. Reference | Related Articles | Metrics Cited: Baidu(21) Select Study on the Composition and Stability of Soil Aggregates with Different Tea Plantation Age LIU Min-ying, ZHENG Zi-cheng, LI Ting-xuan Journal of Tea Science    2012, 32 (5): 402-410.   DOI: 10.13305/j.cnki.jts.2012.05.007 Abstract （347）      PDF（pc） （731KB）（77）       Knowledge map    Save For the purpose of investigating the effect of tea plantation age on the soil aggregates composition and stability, the field data of tea planted area was collected and laboratory analysis was conducted. Results showed that: (1) In the dry and wet treatments dynamic changes were observed in the amount of soil aggregates with the change in clay diameter. It was found that the decreasing trend in the amounts of micro-aggregates was followed a decrease-increase-decrease trend with the decrease in clay diameter under dry treatment, and show a decrease-increase-decrease trend with the decrease in clay diameter under wet treatment. The amounts of micro-aggregates were decreased with the decrease of clay diameter. Results showed that there were no significant effects of tea plantation age on the soil micro-aggregates. (2) When the clay diameter was >5mm, it was found that the increasing trend in the amount of dry and water-stable aggregates was followed an increase decrease trend with the increase of planting time and the maximum value was found after 20~22 years of tea plantation age. At the same time <5mm of clay diameter displayed an opposite trend as compare to >5mm diameter. (3) The maximum value of MWD, GMD of dry-aggregates and water-stable aggregates were found at tea plantation age of 20~22, and the result is opposite for aggregate fractal dimension value (D value) of soil aggregates. The differences among GMD, MWD and aggregate fractal dimension were highly significant under different planting age, which indicated that the tea plantation age showed significant effect on the soil aggregate stability. Reference | Related Articles | Metrics Cited: Baidu(21) Select Study on the Activity of Dark Tea to Gastrointestinal Tumor SONG Lu-bin, HUANG Jian-an, LIU Zhong-hua, HUANG Hao, WANG Kun-bo Journal of Tea Science    2009, 29 (3): 191-195.   DOI: 10.13305/j.cnki.jts.2009.3.002 Abstract （370）      PDF（pc） （215KB）（160）       Knowledge map    Save Stomach cancer and intestinal cancer are the two common diseases of the alimentary canal tumor. HCT-8 and SGC-7901 cell are taken as model to study the activity of Dark Tea to Gastrointestinal Tumor. The results showed that Dark Tea has good inhibitory action to gastrointestinal tumor cell growth. Dark tea contained two active inhibitors for Gastrointestinal Tumor, and the inhibitor for SGC-7901 cell has low polar than the inhibitor for HCT-8. The inhibiting active of Dark Tea to the two cell models maybe comes from manifold physiological activators, and maybe the low polar substances have stronger power. Reference | Related Articles | Metrics Cited: Baidu(21) Select Isolation and Identification of Thermophilic Fungi during the Fermentation of Puer Tea YANG Rui-juan, LU Jie, YAN Liang, YANG Liu-xia, LI Chen-chen, JIANG Shu, SHENG Jun Journal of Tea Science    2011, 31 (4): 371-378.   DOI: 10.13305/j.cnki.jts.2011.04.007 Abstract （332）      PDF（pc） （562KB）（253）       Knowledge map    Save Thermophilic fungi were isolated and identificated by the method of sequencing during traditional fermentation of puer tea. Sampling were carried out every day. Many types of microorganisms including thermophilic fungi were purified and Identified. Isolated thermophilic fungi were cultured at different temperatures and different pH, the growth state of thermophilic microorganisms were observed. This article showed a comprehensive analysis of microorganisms during the fermentation of puer tea, and identified the isolated microorganisms using molecular biology method. Thermophilic microorganisms which play an important role during the fermentation were discovered, it laid the foundation to reveal the mechanism of Puer tea fermentation at high temperature. Reference | Related Articles | Metrics Cited: Baidu(21) Select A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV HOU Ru-yan, ZHU Xu-jun, ZHANG Zheng-zhu, CAI Hui-mei, WAN Xiao-chun Journal of Tea Science    2009, 29 (1): 67-71.   DOI: 10.13305/j.cnki.jts.2009.1.011 Abstract （392）      PDF（pc） （365KB）（202）       Knowledge map    Save A method was developed for determination of imidacloprid residue in tea by high performance liquid chromatography (HPLC) with UV detector. Imidacloprid in tea samples was extracted with methyl alcohol and re-extracted by dichloromethane then cleaned up with the ENVI-18 cartridge. Imdacloprid was eluted with acetonitrile:water(v/v, 30:70) solution from the cartridge and detected with HPLC. The recovery of imidacolprid ranged from 81.5%~87.9% with the RSD less than 10.2%. Practical determination limit was 0.05 mg/kg. Reference | Related Articles | Metrics Cited: Baidu(20) Select Status and Suggestions of Tea Garden Fertilization on Main Green Tea-Producing Counties in Zhejiang Province MA Li-feng, CHEN Hong-jing, SHAN Ying-jie, JIANG Ming-bei, ZHANG Gen-miao, WU Lin-tu, RUAN Jian-yun, LV Jian-fei, SHI Yuan-zhi, PAN Li-xun, HUANG Cheng-mu, LIU Lin-ming, LIANG Bi-yuan, WANG Mei-qin, PAN Jian-qing Journal of Tea Science    2013, 33 (1): 74-84.   DOI: 10.13305/j.cnki.jts.2013.01.010 Abstract （409）      PDF（pc） （871KB）（202）       Knowledge map    Save Fertilization status of typical green tea gardens in Zhuji, Shengzhou, Chun’an, Kaihua, Yongjia, Jiande, Changxing, Quzhou, Shaoxing, Songyang, Wuyi and Fuyang counties, and Xihu district of Zhejiang province in 2009 and 2010 was investigated. The result showed that fertilization of the investigated areas had several characteristics. Firstly, the main applied fertilizers were urea, compound fertilizer and organic manure (including rapeseed and livestock). Secondly, the fertilizers were mainly applied to soil surface in the way of broadcast. and compound fertilizer and organic manure were applied as topdressing. And period and frequency of tea gardens fertilizer application was random. Thirdly, the amount of N applied was deficient for 30% and excessive for 41% of tea gardens. Soil available P content was excessive for 50.4% of tea gardens. And soil available K content was deficient for 48.8% of tea gardens. Finally some fertilization suggestions are recommended according to tea garden application status. Reference | Related Articles | Metrics Cited: Baidu(20) Select Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an, GONG Yu-shun Journal of Tea Science    2005, 25 (3): 225-228.   DOI: 10.13305/j.cnki.jts.2005.03.012 Abstract （420）      PDF（pc） （316KB）（175）       Knowledge map    Save High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector（ELSD-2000）was used for the determination of theanine in tea without derivatization. A Polaris-C18 column (250βmm×4.6βmm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution .The flow rate was at 0.5βml/min. The linear response ranged from 0.062~0.450βmg/mL for theanine. The recovery was 97.5%. The method was evaluated as accurate and simple. Reference | Related Articles | Metrics Cited: Baidu(20) Select Study on the Content of EGCG3"Me in Different Tea Germplasms and its Changes LU Hai-peng, TAN Jun-feng, LIN Zhi Journal of Tea Science    2006, 26 (4): 310-314.   DOI: 10.13305/j.cnki.jts.2006.04.015 Abstract （318）      PDF（pc） （415KB）（156）       Knowledge map    Save The changes of EGCG3"Me content in different tea germplasms, crop seasons and tea processing were studied by HPLC. The content of EGCG3"Me in six tested tea cultivars was more than 1% among 200 tea germplasms containing high tea polyphenols. The content of EGCG3"Me in new tea shoot was increased with the leaf maturity, and was higher in autumn tea than in summer tea. The content of EGCG3"Me was not changed obviously during the process of green tea manufacture, but increased distinctly during the withering process of the Oolong tea. Reference | Related Articles | Metrics Cited: Baidu(20) Select AFLP Analysis on Genetic Diversity of Fenghuang-Dancong Tea Plant Germplasms XIAO Li-zheng, YAN Chang-yu, LI Jia-xian, LUO Jun-wu, HE Yu-mei, ZHAO Chao-yi Journal of Tea Science    2007, 27 (4): 280-285.   DOI: 10.13305/j.cnki.jts.2007.04.002 Abstract （282）      PDF（pc） （714KB）（209）       Knowledge map    Save Genetic diversity on 34 Fenghuang-Dancong tea plants were evaluated by AFLP fingerprinting using 5 primer combinations. The 5 primer combinations generated 438 bands and 87.6 bands were amplified by each primer pair on average, 348 bands(79.3%) polymorphic AFLP markers were detected, The result indicated that the genetic distance(GD) based on AFLP data by using DPS2000 was significant. The varience range of GD among the 34 Fenghuang-Dancong was 0.13~0.49. The GD(0.49) is highest between Zimaohuangzhixiang and Funanmilan and between Baiyedancong and Tongtianxiang, however, the GD(0.13) between Da-ansongcha and Zongsuojia is low. The dendrogram indicated that the genetic of 34 Fenghuang-Dancong germplasms have no certain relation with the aroma type. Reference | Related Articles | Metrics Cited: Baidu(20) Select Study on the Corpulent-reducing Function of Tea GONG Jin-yan, JIAO Mei, WU Xiao-qin, ZHANG Ying Journal of Tea Science    2007, 27 (3): 179-184.   DOI: 10.13305/j.cnki.jts.2007.03.014 Abstract （373）      PDF（pc） （398KB）（275）       Knowledge map    Save Obesity may occur after long period of disorders in appetite and energy metabolism regulations. With the increasing of obesity incidence, searching for the high effective corpulent-reducing medicine became the common wish of research. Tea has been widely used as healthy drink for thousands of years, its function of corpulent-reducing has brought more and more attention all over the world. In this paper, researches of corpulent-reducing function of tea and its mechanism have been summarized. Reference | Related Articles | Metrics Cited: Baidu(19) Select Anticancer Activity of Theaflavin Diagallate and its Mechanism JIANG He-yuan, Hang Xiao, YUAN Xin-yue, WANG Chuan-pi, Chung S Yang Journal of Tea Science    2007, 27 (1): 33-38.   DOI: 10.13305/j.cnki.jts.2007.01.005 Abstract （314）      PDF（pc） （406KB）（393）       Knowledge map    Save Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC50 value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line. Reference | Related Articles | Metrics Cited: Baidu(19) Select Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP YU Mei, JIANG Chang-jun, YE Ai-hua, WANG Zhao-xia, ZHU Lin, DENG Wei-wei, GAO Xuan, WAN Xiao-chun Journal of Tea Science    2007, 27 (3): 259-264.   DOI: 10.13305/j.cnki.jts.2007.03.007 Abstract （324）            Knowledge map    Save For studies of differential gene expression, cDNA-AFLP was analyzed in tea plant at the stage of bud flower development. The result showed that there were evident differential expression in flower between the early stage and the late stage. Of the 1110 fragments inspected, 35 and 87 specially displayed in the early and late stages; 15 fragments were cloned and sequenced. Sequence analysis indicated that among 15 squenced fragments, nine were sequence-similar to proteins in GenBank, i.e. oxidoreductase, chromosome 13, 14-3-3 protein, Amyrel gene, calreticulin interacted protein, ATP sulfurylase, catalytic / iron ion binding, desiccation-related protein and ntp302, two were similar to hypothetical proteins, and no similar sequence were found in GenBank for the other 4 fragment. Reference | Related Articles | Metrics Cited: Baidu(19) Select Taxonomic Study on the Dendrocola of the Tea Plantations in Southern Area of Henan Province LU Dong-sheng, WU Xiao-qin Journal of Tea Science    2004, 24 (4): 243-248.   DOI: 10.13305/j.cnki.jts.2004.04.004 Abstract （314）      PDF（pc） （39KB）（230）       Knowledge map    Save The present paper is to report the classification of fungi on tea bushes in Southern part of Henan Province. Two hundred and thirty four specimens were collected by the author from 13 tea gardens, and 46 species in 27 genera belonging to 13 families have been identified. Among them, 17 species are new records of those which are living on tea bushes in China and 19 species are new records in Henan Province. The distribution and the bioecological habit of every species of the fungi are given. Reference | Related Articles | Metrics Cited: Baidu(19) Select Study on the Changes of Non-galloylated Catechins and Relative Enzymes in Tea Shoots ZHANG Xian-lin, GAO Li-ping, XIA Tao, LIU Ya-jun, GAO Ke-jun Journal of Tea Science    2009, 29 (5): 365-371.   DOI: 10.13305/j.cnki.jts.2009.5.006 Abstract （295）      PDF（pc） （281KB）（143）       Knowledge map    Save The changes of non-galloylated catechins and relative enzymes as well as the composition of catechins in different parts of tea shoots and in different seasons were investigated in this paper. The results showed that the contents of non-galloylated catechins except GC increased gradually from bud to the fourth leaf. Both of two synthetical enzymes related to non-galloylated cahechins, the activity of DFR/LAR declined gradually, but the activity of ANR was the highest in the third leaf. The result of correlation analysis showed that the contents of total catechins in different tea leaves presented a prominent positive correlation with the activity of DFR/LAR,but not with the activity of ANR. Reference | Related Articles | Metrics Cited: Baidu(19) Select Differences in Composition and Dynamic of Insect and Mite Community among Three Types of Tea Gardens HAN Bao-yu Journal of Tea Science    2005, 25 (4): 249-254.   DOI: 10.13305/j.cnki.jts.2005.04.003 Abstract （383）      PDF（pc） （271KB）（169）       Knowledge map    Save From July 2002 to July 2003, a survey on the species richness and abundance of insects and mites in the tea plant, the aerial space above the tea plant and on the ground from organic, non-pollution and common tea gardens were conducted in Magushan Mountains in the Southern Anhui Province. In total, 29018 individuals of 79 species from 41 families were recorded from a common tea garden, 35117 individuals of 81 species from 41 families were recorded from a non-pollution tea garden, and 12727 individuals of 102 species from 57 families were recorded from an organic tea garden. The tea green leafhopper, Empoasca vitis, abundance and its percentage accounted for the total abundance in organic, non-pollution and common tea garden were 5176 and 40%, 14049 and 40% as well as 17590 and 60%, respectively. Wasps, ground beetles, tiger beetles, rove beetles and ladybugs are the major natural enemies in tea gardens, whose species richness and abundance in organic, non-pollution and common tea garden were 40 and 2620, 33 and 1898 as well as 29 and 1610, respectively. In all the three types of tea gardens, species richness was higher from March to April and from September to October, and was lower from late November to middle January. Insect and mite abundance was high from April to June and from September to October, and was lower from July to August due to the warm weather as well as from December to January due to the cold weather. The abundance in the common tea garden fluctuated more than in other two types of tea gardens. Though the Insect and mite abundance was highest in the non-pollution tea garden, the pests did not cause serious damage because of the appropriate forecast and control. In the organic tea garden, the abundance was the lowest, while the species richness was the highest, and the energy paths were complicated and the community may be stable. At the present stage, most of the organic tea gardens in China are established among the mountains where vegetation is flourishing and biodiversity is high, such ecosystems may enhance natural control. Reference | Related Articles | Metrics Cited: Baidu(18) Select The Anti-obesity Effects of Hubei Qingzhuan Tea on Rats CHEN Yu-qiong, ZHANG Wei, CHENG Qian, DONG Fa-jin, LIU Xiao-hua, GAN Duo-ping, QIAN Yu-ping Journal of Tea Science    2008, 28 (5): 363-369.   DOI: 10.13305/j.cnki.jts.2008.05.011 Abstract （435）      PDF（pc） （1319KB）（160）       Knowledge map    Save The anti-obesity effects of Hubei Qingzhuan tea on rats were investigated. The results showed, compared with the obese control group, New Qingzhuan tea of this year(NQT) and the Qingzhuan tea which had been stored for 10 years(QTS) reduced the body weights of rats, Lee’s index and the ratio of body fat and mass significantly. Both teas possessed obvious effects on lowering the serum and liver level of TC, TG, MDA, increasing HDL-C levels and the activities of SOD, GSH-PX. The observation on the fat tissue showed that both teas could reduce the accumulation and volume of fat cells. As a results, New Qingzhuan tea had the effects of anti-obesity and antioxidation. The effects of anti-obesity increased with the dose adding of NQT, and the anti-obesity effects of QTS were better than that of the NQT. Reference | Related Articles | Metrics Cited: Baidu(18) Select Effects of Different Extraction Processes on the Components of Tea Polysaccharides ZHOU Xiao-ling, WANG Dong-feng*, LI Su-zhen, ZHOU Xun, HOU Yang-feng, WANG Yuan-hong Journal of Tea Science    2007, 27 (1): 27-32.   DOI: 10.13305/j.cnki.jts.2007.01.004 Abstract （388）      PDF（pc） （498KB）（212）       Knowledge map    Save Four different treatments including water-extracted process, pectase-extracted process, trypsin-extracted process and combined-enzyme-extracted process were respectively applied to extract tea polysaccharides (TPS) from Laoshan green tea in this article. The extract ratio of combined-enzyme process method reached 5.17±0.17 % and higher than those of the three other process methods. The crude TPS obtained from the four process methods were further purified through Sepharose FF and the main polysaccharides fraction was obtained respectively. Then comparisons on the components of monosaccharides, amino acids in the purified TPS were conducted. The four process methods had no influence on the kinds of monosaccharides in tea polysaccharides, but little on the proportion of the monosaccharisdes. The total sugar content of TPS prepared by the four process methods was decreased in following pectase-extracted process, combined-enzyme-extracted process, trypsin-extracted process, and water-extracted process. Especially, in pectase-extracted process it reached to 95.26±4.09%, while the uronic acids content decreased greatly. The four process methods had no influence on the kinds of amino acids in tea polysaccharides, but great on the proportion of them. Reference | Related Articles | Metrics Cited: Baidu(18) Select Effects of Spreading Environment on Tea Polyphenols and Catechin Components in Tea fresh Leaves of High Quality Green Tea YIN Jun-feng, MIN Hang, XU Yong-quan, YUAN Hai-bo, WEI Kun-kun Journal of Tea Science    2008, 28 (1): 22-27.   DOI: 10.13305/j.cnki.jts.2008.01.002 Abstract （399）      PDF（pc） （303KB）（172）       Knowledge map    Save Using one bud or one leaf and one bud as material, fresh tea leaves were spread continuously (78%~61%) and spread under different environment conditions, then fixed with liquid nitrogen and freeze-dried, respectively. The effects of spread process and spread environments on the change of tea polyphenols and catechin components were investigated. The results showed that the contents of tea polyphenols and total catechins decreased at beginning and then increased during spreading, total esters type catechins decreased gradually, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epicatechin gallate (ECG) decreased in amount during spreading, whilst epicatechin (EC) and epigallocatechin(EGC) increased, but gallocatechin (GC) could not be checked out in fresh tea leaves and spread tea leaves. With the precondition of that the moisture of spread leaves was controlled at 70%, there was obvious effect of environment temperature and humidity on the change of chemical components during spreading. Contents of tea polyphenols and total catechins decreased in low relative humidity (60%), but increased in high relative humidity (90%). The effects of middle relative humidity (75%) on tea polyphenols and total catechins was correlative with environment temperature. Reference | Related Articles | Metrics Cited: Baidu(17) Select Study on the Optimum Conditions in Extracting Polysaccharides in Sichuan Brick Tea GONG Fa-yong, QI Gui-nian, LI Jing Journal of Tea Science    2005, 25 (3): 229-236.   DOI: 10.13305/j.cnki.jts.2005.03.013 Abstract （278）      PDF（pc） （590KB）（151）       Knowledge map    Save By means of orthogonally rotational combination design, a four-factor (temperature, time, ratio between tea and water, pH) substrate cultivation experiment was conducted by using of brick tea. The regression equations of the relationship between temperature, time, ratio between tea and water, pH and extraction rate of tea polysaccharides (TPS) were established. The results indicated that extraction rate of TPS was affected obviously in successive order by pH, temperature, ratio between tea and water and time. In range of experiment, extraction rate of TPS was increased obviously when pH and temperature were in higher levels, and the interaction of pH and temperature to the ratio between tea and water was significant, but the change of time and ratio between tea and water had less influence effects on extraction rate of TPS. Considering the high extraction rate and activity of TPS, the extraction conditions were optimized by frequency analysis that were 74.06~80.74℃temperature, 69.56~79.58βmin time, 38.49~42.59 ratio between tea and water, 7.411~8.284 pH. Reference | Related Articles | Metrics Cited: Baidu(17) Select Pesticide Residues in Tea and Their IntakeAssessment Using Brew Factor Anoop Kumar Barooah, Monorama Borthakur, Jatindra Nath Kalita, Tabaruk Hussain, Kasturi Chaudhury, Rajib Nath Journal of Tea Science    2011, 31 (5): 419-426.   DOI: 10.13305/j.cnki.jts.2011.05.009 Abstract （364）      PDF（pc） （507KB）（229）       Knowledge map    Save Tea, the most popular beverage in the world, is produced from processed leaves of the plant Camellia sinensis. The tea plantations suffer from a large number of pests necessitating use of pesticides. In view of growing concerns for food safety, terminal residues of seven pesticides in processed black tea and their transfer into tea brew was studied. The residues of the pesticides in black tea ranged from 0.18 to 1.90mg/kg. Only a portion of these residues were transferred into tea brew, the form consumed. The actual intakes of residues through tea consumption, determined by incorporating a new parameter termed “brew factor” in the conventional estimates, were only up to 0.1% of the acceptable daily intakes for an average Indian. The compound specific brew factor improves risk assessment and will enable setting realistic maximum residue limits for pesticides in tea. Reference | Related Articles | Metrics Cited: Baidu(17) Select Advances in Researches on Catechins Acylation Modification LIU Xiao-hui, JIANG He-yuan, ZHANG Jian-yong, YUAN Xin-yue, CUI Hong-chun Journal of Tea Science    2009, 29 (1): 1-8.   DOI: 10.13305/j.cnki.jts.2009.1.001 Abstract （339）      PDF（pc） （287KB）（310）       Knowledge map    Save Researchers and the general public have been paid more and more attention to catechins due to their distinctive and extensive bioactivities, but the further applications are restricted because its liposoluble is not high, unstability and low bioavailability in vivo. This paper reviewed the research progress of acylation modificated catechins and their physiological activities as well as the prospects of development． Reference | Related Articles | Metrics Cited: Baidu(17) Select Progress in the Research of Biosynthesis of Volatile Terpenoids and Their Glycosides in Tea Plant HE Zhi-rong, XIANG Wei, XU Yan, GAO Li-ping, XIA Tao, WEI Shu Journal of Tea Science    2012, 32 (1): 1-8.   DOI: 10.13305/j.cnki.jts.2012.01.008 Abstract （573）      PDF（pc） （690KB）（226）       Knowledge map    Save Tea aromatic quality is largely dependent on the spectrum and abundance of volatile terpenoids and their glycosides produced and accumulated in tea leaves. Very often, the compounds of monoterpenes (C10) and sesquinterpene (C15) possess pleasant floral scent, contributing significantly to tea aromatic quality. However, studying on the biosynthesis pathway of these volatile terpenoids and their glycosides in tea plant is just at beginning. In this review, speed-limiting biosynthetic steps and related enzymes in plant volatile terpenoid biosynthesis pathways are summarized. The genes encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and 3-hydroxyl-3-methylglutaryl-CoA reductase (HMGR) as well as terpenoid synthases are proposed as key enzymes for volatile terpenoid biosynthesis. Uridine diphosphate-glycosyltransferase may affect biosynthesis and accumulation of glycosides of volatile terpenoids in tea plant while glycosidase play a key role in the glycoside hydrolysis and release of glycosidically bound terpenoid volatiles during tea leaf processing. Manipulation of these genes may control the flux of the terpenoid metabolism towards the volatile terpenoid biosynthesis. The factors influencing tea aromatic quality such as tea cultivars, growing management, processing ways are also discussed. Reference | Related Articles | Metrics Cited: Baidu(17) Select Research Advance on Tea Biochemistry WAN Xiaochun, LI Daxiang, ZHANG Zhengzhu, XIA Tao, LING Tiejun, CHEN Qi Journal of Tea Science    2015, 35 (1): 1-10.   DOI: 10.13305/j.cnki.jts.2015.01.002 Abstract （757）      PDF（pc） （1093KB）（544）       Knowledge map    Save Tea biochemistry is a subject of life science on Camellia Sinensis. It mainly focus on characteristic secondary metabolites of tea plants, especially those in the fresh tea shoots using biochemical and molecular techniques as research tools. Those metabolites include catechins, caffeine, theanine and volatile terpenes, which contribute greatly to tea quality and healthy effects. The major research fields of tea biochemistry involve the biosynthesis of secondary metabolites in tea plants, their transformation during tea processing and their effects on final tea quality and healthy function. Recently, there have several breakthroughs in the metabolites biosynthesis pathway, tea genome, specific tea germplasm metabolome, the processing metabolome, as well as the chemistry of tea quality. Since tea biochemistry is the fundamental in tea science, its achievements provide the theoretical basis and method approaches to tea cultivation and breeding, tea primary and further processing, tea trading and tea culture. With the development of tea industry and life science & technology, it is believed that the advancement of tea biochemistry will push forward tea industry to a high level at the sustainable development. Reference | Related Articles | Metrics Cited: Baidu(17) Select A Review on the Regulation Technique of Withering Process and the Change in Physical and Chemical Properties of Leaves HUA Jin-jie, YUAN Hai-bo, JIANG Yong-wen, LIU Ping, WANG Wei-wei Journal of Tea Science    2013, 33 (5): 465-472.   DOI: 10.13305/j.cnki.jts.2013.05.005 Abstract （475）      PDF（pc） （569KB）（489）       Knowledge map    Save The withering is an important process of fermented tea, such as black tea and oolong tea, significantly affected the sensory quality of tea. With water constantly lost in the withering process, the respiration of fresh leaf is carrying on continuously, the endoplasmic components are also dramatically changed and the physical characteristics of withering leaves are also prompted to change. All of these provided specific substances and leaves basis for the follow-up processes and the quality of finished tea. This paper illustrates the dynamic variation of the physical properties and biochemical components in the withering process, and conducted a systemic review on the regulation and control technology on the key parameters of current withering. The facility withering technology, which can accurately control these parameters including environment conditions, such as temperature, humidity, light conditions and aeration conditions, will become the important direction of withering development in the future. Reference | Related Articles | Metrics Cited: Baidu(16) Select ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province LIU Ben-ying, WANG Li-yuan, LI You-yong, TANG Yi-chun, HE Wei, CHENG Hao, WANG Ping-sheng Journal of Tea Science    2009, 29 (5): 355-364.   DOI: 10.13305/j.cnki.jts.2009.5.005 Abstract （304）      PDF（pc） （598KB）（163）       Knowledge map    Save It was of much importance using minimum primers to obtain the maximum identification ability．Molecular identification of 134 tea germplasm resources using inter-simple sequence repeat (ISSR) markers was conducted in this study. There were 3 independent ways to identify tea germplasms: a) unique ISSR markers;b) unique band patterns and c) a combination of the band patterns provided by different primers．The result showed that the presence of 10 unique ISSR markers and the absence of 15 unique markers obtained from 12 primers made it possible to identify 21 tea germplasms, including ‘Xiangzhuqingdashancha’ and so on. Using 54 unique band patterns of primer UBC811 could identify 35 tea germplasms, including ‘Hainandayecha 1’ and so on. A1l the 134 tea germplasms could be entirely identified by the band patterns combination of primer UBC811, UBC835, ISSR2 and ISSR3, which was successfully used to construct the ISSR fingerprinting for discriminating 134 tea germplasms from Yunnan Province. Reference | Related Articles | Metrics Cited: Baidu(16) Select Studies on the Optimal Water Condition for Tea Seedlings under Greenhouse Manufacturing Administration ZENG Jian-ming, GU Bao-jing, CHANG Jie, YUAN Hai-bo, WANG Li-yuan, DONG Fang-shuai, CHENG Hao, ZHOU Jian, GE Ying, Chen Shenglun Journal of Tea Science    2005, 25 (4): 270-274.   DOI: 10.13305/j.cnki.jts.2005.04.006 Abstract （445）      PDF（pc） （286KB）（155）       Knowledge map    Save The photosynthesis, chlorophyll content and the growth traits of the clonal tea seedling grown under various substrates with different water content under the greenhouse were compared. The new biomass reaching their maximum values in the substrate with 80%~100% relative substrate water content, root mass ratio (RMR) decreased with the increase of water content of substrate, while the content of chlorophyll, specific leaf area (SLA), leaf area ratio (LAR), root emerging rate of the plant and specific root length (SRL) increased as the water content of substrate increased. The maximum photosynthetic rate (Pnmax) and new biomass increased as the water content of substrate increased, reaching their maximum values at 70%~100% water content of substrate. Content of chlorophyll increased with the rise of relative water content of substrate, while the ratio of chlorophyll a/b decreased with the substrate water content of substrate. Lower substrate water content can expedite the growth of tea seedling after rootage. Reference | Related Articles | Metrics Cited: Baidu(16) Select Research on the Correlative Factors of Sensory Quality of High-quality Green Tea WANG Tong-he, HU Min, ZHANG Jiu-qian, LI Hong Journal of Tea Science    2008, 28 (1): 33-38.   DOI: 10.13305/j.cnki.jts.2008.01.005 Abstract （392）      PDF（pc） （264KB）（187）       Knowledge map    Save The factors of tea sensory evaluation by biological statistical software SAS9.0 were conducted according to the results of sensory evaluation from Tea Culture Festival in Xinyang city from 2004 to 2007. Results showed that the correlation between liquor color and aroma, taste, infusion was significant, but the correlation between appearance and the other factors was not significant. It was showed that the taste﹥aroma﹥liquor color﹥infusion﹥appearance in comparing with total quality score. The score proportion of color 23, aroma 23, taste 24, appearance 30 was rather reasonable. The correlative equation of each factor and total quality score was established by the method of stepwise regression with the DPS data processing software as follows: Y=25.8852+0.731X1+0.702X2+1.02X3+1.2204X4+1.03X5 Reference | Related Articles | Metrics Cited: Baidu(16) Select The Demonstration Research on Congregation Degree of Tea Industry SONG Yan-ping, WANG Yan-rong Journal of Tea Science    2008, 28 (5): 379-385.   DOI: 10.13305/j.cnki.jts.2008.05.012 Abstract （320）      PDF（pc） （314KB）（169）       Knowledge map    Save Industry accumulation is an economic phenomenon of space congregation or local special congregation with the process of economic development. The paper calculates the congregation degree of AnHui tea industry in the period of 2002~2006 by using the means of LQ quotient and the index of concentration. The result indicated that Anhui tea industry possessed a signification congregation tendency and it’s mostly concentrated in the south and west regions of Anhui province. The result also indicated that the level of AnHui tea industry accumulation has a strong positive correlation to the industry development. Reference | Related Articles | Metrics Cited: Baidu(16) Select Evaluation of Nitrogen Status in Tea Plants by SPAD YANG Yi-yang, MA Li-feng, SHI Yuan-zhi, RUAN Jian-yun, LI Xing-hui Journal of Tea Science    2008, 28 (4): 301-308.   DOI: 10.13305/j.cnki.jts.2008.04.007 Abstract （365）      PDF（pc） （525KB）（242）       Knowledge map    Save Determination of nitrogen concentrations in tissues is an important diagnostic means for N status of plants. A pot experiment with tea plants applied with different amounts of N fertilizer was carried out to evaluate the usefulness of SPAD-502 as a diagnostic tool. The results showed that the yield of young shoots and N concentration of mature leaves responded to N application rates with a pattern of an initial linear increase before a platform was reached. There was a significantly positive linear relationship between the SPAD reading and total N concentration of mature leaves. The similar pattern of an initial linear increase followed by a platform was observed for the relationship between SPAD reading and the yield. These results suggested that the N status of tea plants may be characterized by the measurement of SPAD reading of mature leaves and the SPAD is a potentially useful rapid diagnostic tool in N fertilization recommendation. However, this needs further validation from field experiments. Those factors which may influence the precision of SPAD measurement, such as the point of test on a leaf, selection of leaves from twig (position), weather condition, in situ or in vitro analysis, surface cleaning and the number of leaves for a composite sample were also investigated. Reference | Related Articles | Metrics Cited: Baidu(16) Select A Survey on the Trapping Effect of Four Types of Coloured Sticky Plates on Various Species of Insects in Tea Gardens XIANG Tai-hong, HAN Bao-yu, ZHOU Xiao-gui Journal of Tea Science    2007, 27 (3): 253-258.   DOI: 10.13305/j.cnki.jts.2007.03.005 Abstract （327）      PDF（pc） （394KB）（164）       Knowledge map    Save In order to evaluate the entrapping effect of the bud green, the olive yellow-green, the jasmine yellow and the snow white sticky plates on various insects in tea gardens and to discuss the possibility for them to be applied in controlling the important pests, the luving tests, were conducted. In July when the various species of insects occurred in tea gardens, the plates were set in 0.5βhm2 tea gardens to trap the insects for 48βh, in the tea gardens of Chongqing Suburbs, in Songyang County of Zhejiang Province and in Magushan Tea Farm of Anhui Province, respectively. The results showed that the most of trapped insects belonged to Homoptera, in which the attracted amounts of harmful insects was abundant whilst the those of beneficial insects was much small, and the difference in both amounts reached the level of P<0.01. Bud green, olive yellow-green, jasmine yellow and snow white sticky plates showed much stronger trapping effect on nymphs and adults of tea green leafhoppers and adults of citrus spiny whiteflies than snow-white stiky plates. From bud green, olive yellow-green to jasmine yellow, green ingredient decreased whilst yellow ingredient increased, accordingly, the number of trapped leafhoppers decreased whilst the number of trapped whiteflies increased. It is proposed that bud green and jasmine yellow sticky plates could be used to trap the leafhoppers and the whiteflies, respectively. Reference | Related Articles | Metrics Cited: Baidu(16) Select Study on Blending of Made Tea with Mixture Design TONG Hua-rong, GONG Zheng-li Journal of Tea Science    2004, 24 (3): 207-211.   DOI: 10.13305/j.cnki.jts.2004.03.011 Abstract （329）      PDF（pc） （102KB）（187）       Knowledge map    Save Blending program of 7 scented teas using Uniform mixture Design of experiments was studied. Results showed that Uniform mixture design of experiments could be used in blending of made tea effectively. With the model of responses, the interactions of sensory attributes among components could be estimated. With the aid of analysis software HL40, formulations of tea could be optimized for several responses and the best formulation could be gotten. Reference | Related Articles | Metrics Cited: Baidu(16) Select HPLC Quantitation of Theaflavins in Tea Pigments LI Da-xiang, WAN Xiao-chun, LIU Li-hua, XIA Tao Journal of Tea Science    2004, 24 (2): 124-128.   DOI: 10.13305/j.cnki.jts.2004.02.010 Abstract （367）      PDF（pc） （274KB）（280）       Knowledge map    Save In this study, theaflavin(TF1), theaflavin monogallate(TF2+TF3) and theaflavin digallate(TF4) in tea pigments are isolated and purified with high speed counter current chromatography combined with Sephadex LH-20 and semi-preparative HPLC. With the purified theaflavin, the standard curves have been set up by HPLC for theaflavins quantitation. The results on the accuracy and recovery determination showed that the coefficient of variation of TF1, TF2+TF3, TF4 and total theaflavins range from 2.6’8.5%, 2.0’3.8%, 2.0’3.9% and 2.2’4.1% respectively. Using HPLC quantitation, the relative error of total theaflavins amounts in the standard theaflavins from Sigma company is 3.4%. The total amounts of theaflavins in tea pigments are 20.5%, in which TF1, TF2+TF3 and TF4 are 6.0%, 9.1% and 5.4% respectively. This method is useful to quantify theaflavins and to control the quality of tea pigments, which could promote the studies on their pharmacological effects. Reference | Related Articles | Metrics Cited: Baidu(16) Select Analysis of the Xanthophyll Cycle Component in Fresh Leaves of Tea Plant by High Performance Liquid Chromatography and Its Photo-protection WEI Chao-ling, JIANG Chang-jun, TAO Han-zhi, HU Ying-hui Journal of Tea Science    2004, 24 (1): 60-64.   DOI: 10.13305/j.cnki.jts.2004.01.012 Abstract （297）      PDF（pc） （307KB）（144）       Knowledge map    Save The xanthophyll cycle components in fresh leaves of tea plant (Camellia sinensis cv. Fudingdabai)were extracted by acetone. The pigments were analyzed with a Spherisorb column （C18 , 5μm,250㎜×4.6βmm）,and were eluted using acetonitrile: methanol(85:15) and methanol:ethyl acetate(68:32) by a linear gradient. The pigments were detected by their absorbance at 445βnm. It was showed that violaxanthin, antheraxanthin and zeaxanthin from fresh leaves of tea plant were detected by HPLC, respectively, under temperatures of 15℃, 22℃ and 30℃, meanwhile, the pigments were separated well, and it is a sensitive, well-reappeared method. Tea plant had a larger xanthophyll cycle pool and a larger de-epoxidation state in higher sun-light, which indicated xanthophyll cycle plays an important role in photo-protection in tea plant. Reference | Related Articles | Metrics Cited: Baidu(16) Select Effects of Vacuum Freezing Dry on the Aroma Quality of Oolong Tea YE Nai-xing, YANG Ru- xing, YANG Guang, YANG Jiang-fan, LIANG Xiao-xia, ZHENG De-yong Journal of Tea Science    2006, 26 (3): 181-185.   DOI: 10.13305/j.cnki.jts.2006.03.005 Abstract （481）      PDF（pc） （411KB）（137）       Knowledge map    Save The vacuum freezing dry technique was applied during the drying process of the faint-scent type oolong tea. Compared with the freezing tea and baked tea, the contents of linalool, aromadendrene, β-caryophyllene, isovaleric acid phenethyl ester, β-cadinene, nerolidol, farnesol and hexatriacontane were higher in the freezing-dry tea. The vacuum freezing-dry technique could help to preserve the properties of color, aroma, flavor of oolong tea efficiently so that the freezing-dry tea had a strong flower scent, and its mellow was full of flavor. Therefore, the freezing-dry tea abowed a better quality in general compared with freezing tea and baked tea and would have a good prospective in the market in addition to its long storage and convenience in transportation. Reference | Related Articles | Metrics Cited: Baidu(15) Select Effects of Zuoqing Intensity on the Protein Composition, Polyphenol Oxidase and Esterase Isozymogram of the Fresh Leaves during Oolong Tea Manufacture HUANG Fu-ping, LIANG Yue-rong, CHEN Rong-bin, CHEN Wei, KE Yu-qin, LU Jian-lian Journal of Tea Science    2004, 24 (1): 70-74.   DOI: 10.13305/j.cnki.jts.2004.01.014 Abstract （301）      PDF（pc） （486KB）（223）       Knowledge map    Save Using Rougui (Camellia Sinensis (L.) var.) fresh leaves as material, fresh leaves soluble protein content and its composition by SDS-PAGE, polyphenols oxidase activity and its isozymogram,and esterase isozymogram were investigated during Oolong tea Zuoqing procedure with different Zuoqing intensity. The results indicated that, during zuoqing process, the soluble protein content and its protein components decreased, and decreased sharply with the intensity, during the periods polypenol oxidase activity increased at the earlier stage, and decreased at the later, its isozymogram checked out 7 bands, no new bands was found during the period, and its E2 band activity apparently enhanced while its E6 activity decreased. Esterase had 7 isozymogram bands, the changes of the each band activities was not remarkable in the whole procedure. The relations of the factors mentioned above to the Oolong quality formation also were discussed in the paper. Reference | Related Articles | Metrics Cited: Baidu(15) Select Progress on the Modification of Tea Polyphenols and Antioxidant Properties of Lipid-soluble Tea Polyphenols YING Le, ZHANG Shi-kang, WANG Yue-fei, ZHU Yue-jin, YANG Xian-qiang Journal of Tea Science    2010, 30 (S1): 511-515.   Abstract （298）      PDF（pc） （180KB）（249）       Knowledge map    Save Producing good quality lipid-soluble tea polyphenols (LTP) was the research focus at home and abroad. Tea polyphenols’ modification by solvent, emulsion and molecular modification were reviewed in this paper. The advantages and disadvantages among these three methods were compared. The antioxidant properties of liphophilic tea polyphenols and its mechanism were introduced. Its antioxidant properties in different antioxidant systems were compared. And the application of LTP as antioxidants was forecasted. At the same time, the developing direction of LTP production was proposed. Reference | Related Articles | Metrics Cited: Baidu(15) Select A Review on the Technique of Tea Decaffeination XU Yong-quan, YIN Jun-feng, YUAN Hai-bo, CHEN Jian-xin, WANG Fang Journal of Tea Science    2008, 28 (1): 1-8.   DOI: 10.13305/j.cnki.jts.2008.01.001 Abstract （461）      PDF（pc） （367KB）（374）       Knowledge map    Save It is reported that tea has many medical functions. However, consumers prefer decaffeinated tea and tea products due to caffeine’s negative disadvantageous. Decaffeination is realized by different methods, including the traditional methods such as water decaffeination, solvent extraction, adsorption separating and supercritical carbon dioxide extraction. The recent developmental microbial and enzymatic degradation and breeding low caffeine tea varieties are also developed for removal of caffeine. The investigation on tea decaffeination was reviewed, and the feasibility was discussed in this paper. The techniques of water decaffeination combined with adsorption separating, microbial and enzymatic degradation as well as low caffeine tea breeding were regarded as the main developmental direction of tea decaffeination by the authors. Reference | Related Articles | Metrics Cited: Baidu(15) Select Change of Polysaccharide During the Processing of Oolong Tea and its Structure Characteristic NI De-jiang, CHEN Yu-qiong, YU Zhi, ZHANG Yun, XIE Bi-jun, ZHOU Ji-rong Journal of Tea Science    2005, 25 (4): 282-288.   DOI: 10.13305/j.cnki.jts.2005.04.008 Abstract （353）      PDF（pc） （363KB）（296）       Knowledge map    Save During the process of Oolong tea, the extraction rate of TPS and the contents of its neutral sugar and protein decreased, particularly notably after the third shaking, but the content change of uronic acid was less. It was also indicated that the inhibiting effects of polysaccharide on radicals increased before the third shaking, and then decreased on Zuoqing stage. According to the changes of polyphenol content, when it was retained 85%, the content of TPS was highest and the effect on scavenging •OH and was best, which was regarded the technical index to end shaking during the process of Oolong tea. Four fractions of OTPS was obtained by DEAE-52 column chromatography, and there were obvious differences not only in contents of neutral sugar, uronic acid and protein of OTPS, but also in inhibiting effects on hydroxyl radical (•OH) and superoxide anion radical ( ). OTPS 2-1, a main fraction of OTPS, was obtained by DEAE-52 and Sephadex column chromatography. It was found that OTPS2-1 was a trinal compound of polysaccharide containing rich uronic acid and less protein. The results also showed that the molecular average-weight of OTPS2-1 was 8.877?104, and it was composed of Gal, Glu, Ara, Fuc and Rha with a molar ratio of 7.58:2.14:7.05:1.76:1.02. Reference | Related Articles | Metrics Cited: Baidu(15) Select HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes JIANG He-yuan, KE Chang-qiang, WANG Chuan-pi, CHEN Xiao-qiang, YUAN Xin-yue Journal of Tea Science    2005, 25 (4): 289-294.   DOI: 10.13305/j.cnki.jts.2005.04.009 Abstract （376）      PDF（pc） （304KB）（157）       Knowledge map    Save Through HPLC and preparing HPLC and the analysis of molecular weight and chemical group by HPLC/MS, Two flavonol glycosides from tea seed flakes were separated and identied. The systematical method combining analytical and preparation scale HPLC with HPLC/MS, could also be used in the study of other natural products in tea. Reference | Related Articles | Metrics Cited: Baidu(15) Select Study on the Variation of Cold Resistance of Tea Plant in Shandong Province SUN Zhong-xu, LIU Jing, QIU Zhi-lin, ZHAO Shu-ping, ZANG Ling Journal of Tea Science    2003, 23 (1): 61-65.   DOI: 10.13305/j.cnki.jts.2003.01.013 Abstract （271）      PDF（pc） （293KB）（131）       Knowledge map    Save Clustering analysis of short distances on tea areas of ten cities and towns and six meteorologic factors showed that tea in Shandong is distributed mainly in four ecological regions. There are Rizhao and Qingdao, Linyi, Junan and Jiaonan of Qingdao; Taian and Xintai; as well as Laoshan of Qingdao and Zhucheng of Weifang. Analysis of variation indexes on tea in each ecological areas showed that frozen injury from light to heavy on tea dispersed piebald-style. Analysis of typical relativity showed that each meteorological factor is positively correlated with the change of tissue structure in tea leaf, among these meteorological factors, the most important factors on tea variation are annual rainfall and the extreme lowest temperature, followed by average annual temperature , frost-free period and sunshine hour, January average annual temperature in January showed little influence on it. With leaf structure the thickness of palisade tissue is most sensitive to climate, then the ratio of palisade tissue to spongy tissue and the thickness of spongy tissue. The thickness of leaf and the upper epidermis showed no relationship with the meteorologic factors. Reference | Related Articles | Metrics Cited: Baidu(15) Select Effect of Airflow on Zuoqing Environment and Quality of Oolong Tea JIN Xin-yi, WANG Xiu-ping, JI Ke-wen, MA Cui-lan, BAO Gui-lin Journal of Tea Science    2003, 23 (1): 41-45.   DOI: 10.13305/j.cnki.jts.2003.01.009 Abstract （337）      PDF（pc） （415KB）（182）       Knowledge map    Save In order to prove up the effect of airflow on Zuoqing environment、physiological change of leaves and quality of Oolong tea, we determine on-line the air parameters including the air temperature, relative humidity, air quality and respiration rate of leaves under two air-conditioned Zuoqing environment respectively simulating direct pressure and all-around blast or airtight one during Zuoqing course. The experiment indicates that the air relative humidity and content of CO2 of leaf-layers and respiration rate of leaves under two airflow conditions showed ultimately marked difference. The quality of Oolong tea made under the blast environment is superior to that made under the other one. Reference | Related Articles | Metrics Cited: Baidu(15) Select An Analysis Method Study of Sequential Extraction and Separation of the Chemical Form of Lead in Tea Garden Soil SHI Yuan-zhi, KANG Meng-li, MA li-feng, HAN Wen-yan Journal of Tea Science    2005, 25 (1): 23-29.   DOI: 10.13305/j.cnki.jts.2005.01.005 Abstract （250）      PDF（pc） （359KB）（230）       Knowledge map    Save With modified sequential extraction and separation analysis method, lead in tea garden was divided into six fractions: water soluble, exchangeable, bound to carbonates, bound to Fe-Mn oxides, bound to organic matter, and residual. All the six forms of lead and total lead were determined with ICP-OES, showing that this method has good precision ,accurate, reliable and easy to operate. Reference | Related Articles | Metrics Cited: Baidu(15) Select Taste Characteristics of Purple Tea Leaf and the Reduction of Bitterness and Astringency ZHAO Xian-ming, WANG Xiao-shi, DU Xiao Journal of Tea Science    2009, 29 (5): 372-378.   DOI: 10.13305/j.cnki.jts.2009.5.007 Abstract （333）      PDF（pc） （347KB）（171）       Knowledge map    Save The quality of the green tea made from purple tea leaf, which is common in tea production, is not so good for its evident bitterness and astringency. In this paper, the main bitterness and astringency characteristic components of purple tea leaf were separated and determined, and the taste characteristics of those components were quantitatively evaluated by the threshold and index of bitterness and astringency. The results showed that the content of anthocyanidins was as high as 1.14%, and the contents of anthocyanidins and catechins of purple tea leaf were 57 and 1.13 times to those of green tea leaf, their higher content was mainly responsible to the bitterness and astringency of purple tea leaves. The order of threshold of those components was caffeine (0.30) < anthocyanidins (0.40) < catechins (0.80), and the order of bitterness and astringency index was catechins (3.32) > caffeine (2.13) > anthocyanidins (0.57), which was also used to evaluate the effect of components on the taste of tea. Furthermore, adding 0.2% casein into purple tea leaf during green tea processing could reduce the bitterness and astringency, the quality could be promoted, the concentration of tea polyphenols in tea infusion could reduce 19.5%, and the bitterness and astringency index decreased from 3.70 to 2.98. The research provided a technological method to reduce the bitterness and astringency of purple tea leaves. By using the polyphenol-protein complex reaction model, the relative precipitation rate (RA) was used as the index to evaluate the inhibiting ability of casein to the bitterness and astringency of polyphenols. Reference | Related Articles | Metrics Cited: Baidu(15) Select Study on Food Functionality of Tea Polysaccharides LI Lei, WANG Dong-feng, ZHOU Xiao-ling, DING Qing-bo Journal of Tea Science    2006, 26 (2): 102-107.   DOI: 10.13305/j.cnki.jts.2006.02.005 Abstract （374）      PDF（pc） （318KB）（176）       Knowledge map    Save Two contents of tea polysaccharides, TPSⅠand TPSⅡ,whose content is separately 27.4% and 57.8%, were extracted from tea, and its food functionalities, such as solubility, capacities of oil absorption, moisture absorption and retention, frothing, froth maintenance and apparent viscosity, were investigated. Results showed that TPSⅠ and TPSⅡ could dissolve in water and not in organic solvents of high concentration, and they had capacities of oil absorption, frothing and froth maintenance as well as capacities of moisture absorption and retention in humid and dry environment. With the increasing of content of polysaccharides in TPS, apparent viscosity of TPS increased; and viscosity of TPSⅠ was four times as much as that of TPSⅡ. Reference | Related Articles | Metrics Cited: Baidu(15) Select Proteomic Analysis of Tea Leaf under Polyethylene Glycol Stress GUO Chun-fang, SUN Yun, LAI Cheng-chun, ZHANG Mu-qing Journal of Tea Science    2009, 29 (2): 79-88.   DOI: 10.13305/j.cnki.jts.2009.2.001 Abstract （333）      PDF（pc） （778KB）（161）       Knowledge map    Save Two-dimensional electrophoresis(2-DE) were carried out to study the proteomic change in of tea leaves in responsive to polyethylene glycol(PEG) stress, and expression profiles of 18 different proteins were analyzed under PEG stress. Sixteen different proteins were identified by MS/MS using 4700 Proteomics Analyzer(Applied Biosystems, USA),and 14 proteins were identified and represented 10 kinds of proteins. In these proteins, 5 were the same protein—ribulose 1, 5-bisphosphate carboxylase/oxygenase large subunit(RubisoLSU), other 9 proteins were response proteins to PEG stress including photosystem II 23 kDa polypeptide,chloroplast chaperonin 21, triose phosphate isomerase(TPI), ATP synthase subunit, S-adenosylmethionine synthetase 1(SAMS1), centroradialis(CEN), nascent polypeptide associated complex alpha chain(α-NAC), 20 S proteasome alpha subunit E2(PAE2) and translation initiation factor 5A(eIF5A). These proteins will play an important role in many life activities of plant, such as chloroplast composition, sugar metabolism, energy metabolism, sulfur metabolism, protein metabolism, signal transduction, gene expression regulation and programmed cell death. Reference | Related Articles | Metrics Cited: Baidu(15) Select Tendency to Different Colors by Ricaniidae Insects in Tea Garden SUN Xiao-ling, CAI Xiao-ming, WANG Guo-chang, GAO Yu, WANG De-qiang, CHEN Zong-mao Journal of Tea Science    2011, 31 (2): 95-99.   DOI: 10.13305/j.cnki.jts.2011.02.003 Abstract （416）      PDF（pc） （338KB）（167）       Knowledge map    Save The phototaxis is the basic behavior of numerous insects. Different species of insects has different preference of spectral range. Eighteen different colors were used to test the preference of Ricaniidae adults in the tea plantation. The results showed that yellow cards with high saturation were the first preference of Ricaniidae adults. Further investigation about field control efficacy of yellow sticky cards should be developed. Reference | Related Articles | Metrics Cited: Baidu(14) Select The Influence on Quality of Pseudosciaena Crocea Dip by Tea Polyphenol during Cold Storage ZHANG Xu-guang, LI Ting-ting, ZHU Jun-li, LI Jian-rong, LI Yu-jin Journal of Tea Science    2011, 31 (2): 105-111.   DOI: 10.13305/j.cnki.jts.2011.02.005 Abstract （330）      PDF（pc） （498KB）（189）       Knowledge map    Save The effects of tea polyphenols (0.2%, w/V) mixture dip treatment on quality of Pseudosciaena crocea during cold storage were examined over a period index of 25d. Sensory characteristics, microbiological (total viable count), and chemical (pH, TVB-N, TBA, K-value) were assessed periodically for the treated group and the control. The results indicated that the shelf life were increased 7~8d at the storage of 4℃ under the tea polyphenols treatment. At the end of shelf life (15d), the total viable count was 106.12CFU/g and 107.45CFU/g, the pH value was 6.78 and 6.84, TBA was 0.12mg/kg and 0.16mg/kg, TVB-N was 166.6mg/kg and 318.7mg/kg, and K value was 27.10% and 43.72%, respectively. Therefore, tea polyphenols showed a significant inhibition effects on the propagation of bacteria and anti-oxidation of fat, thus prolonged the preservation time of Pseudosciaena crocea. Reference | Related Articles | Metrics Cited: Baidu(14) Select Analysis of Fatty Acid Composition in the Seed Oilfrom Fujian Tea Germplasm CHANG Yu-xi, ZHENG De-yong, YE Nai-xing, YANG Jiang-fan Journal of Tea Science    2012, 32 (1): 22-28.   DOI: 10.13305/j.cnki.jts.2012.01.011 Abstract （306）      PDF（pc） （732KB）（151）       Knowledge map    Save The fatty acid compositions of 42 cultivars of tea seeds oil from Fujian province were determined by GC. The results indicated that the average content of saturated fatty acid (SFA) was 20.59%, the monounsaturated fatty acid (MUFA) was 50.57%, and the PUFA was 28.11%. The DHA with a mean of 0.11% was discovered in tea seeds. The investigated fatty acid compositions of fine cultivars were better than that in local cultivars. The major compositions of tea seeds oil were stearic acid, arachidic acid, palmitic acid and DHA. When 11 kinds of higher contents and special compositions were chosen as principal component analysis. The results showed that tea seeds oil possesses a high level of oleic acid, linoleic acid, and low levels of erucic acid and TFA. The tea seeds showed a potential value in the development of high grade edible oil. Reference | Related Articles | Metrics Cited: Baidu(14) Select Science Innocation and Sustainable Development of Tea Industry CHEN Zong-mao, SUN Xiao-ling, JIN Shan Journal of Tea Science    2011, 31 (5): 463-472.   DOI: 10.13305/j.cnki.jts.2011.05.008 Abstract （370）      PDF（pc） （716KB）（227）       Knowledge map    Save After glancing over the information on the development of world tea industry in the period of 1910~2009 and the development of China tea industry in the period of 1950~2010, the relationship between the development of world tea industry and China tea industry as well as the development of tea science and technology was analyzed. Research discovered that those years with more rapid development of science and technology and more creating achievements were accompanied with the rapid development of tea industry. Lists of some important influencing achievements in the development of tea industry were enumerated in this paper. Based on these, seven gaps were discussed in comparing to the world major tea producing countries. In discussing on the future sustainable development of China tea industry, six strategic scientific problems influencing the sustainable development were put forward. Lastly, six tentative ideas including the modern biological technology and traditional breeding technique, the introduction of environmental science and ecological science into the tea production were advanced for the scientific creation of tea industry in the future 10~15 years in this paper. Reference | Related Articles | Metrics Cited: Baidu(14) Select Effects of Fluoride on the Metabolism of Tea Quality Components LI Qiong, RUAN Jian-yun Journal of Tea Science    2009, 29 (3): 207-211.   DOI: 10.13305/j.cnki.jts.2009.3.005 Abstract （355）      PDF（pc） （155KB）（184）       Knowledge map    Save Soil experiment was conducted to investigate the impact of fluoride on yield of young shoots and chemical ingredients related with tea quality. Production of young shoots was improved by modest increase in the soil fluoride levels while significantly decreased by higher F levels. The concentrations of total polyphenols, caffeine and catechins (EGCG, etc) were reduced by fluoride application. The concentrations of total free amino acid and some major components (theanine, glutamate and glutamine) in the young shoots were also decreased significantly by the highest F application. Activity of phenylalanine ammonia-lyase (PAL) and glutamate synthase (GOGAT) decreased with increasing fluoride levels. The results indicated that, though tea plant growth was improved by low level of fluoride, high levels of this element were detrimental to both growth and metabolism of chemical components such as catechins, caffeine and free amino acids. Reference | Related Articles | Metrics Cited: Baidu(14) Select Bacterial Abundance of Tea Garden Soils and Its Influencing Factors HAN Wen-yan, WANG Wan-meng, GUO Yun, YANG Ming-zhen, JIA Zhong-jun Journal of Tea Science    2013, 33 (2): 147-154.   DOI: 10.13305/j.cnki.jts.2013.02.013 Abstract （341）      PDF（pc） （779KB）（115）       Knowledge map    Save Bacterial abundances in tea garden and their adjacent forest and vegetable soils were investigated by real-time quantitative PCR (qPCR) as well as the factors that may affect the population size of bacterial communities. Soil DNA was extracted by using Griffiths’ method and bacterial abundance was determined by quantifying the copy number of 16S rRNA genes. The results showed that the bacterial abundance of tea garden soils ranged from 0.01×108 to 20.32×108 16βS rRNA gene copies/g (gram dry weight soil) with an average of 3.70×108 16βS rRNA gene copies/g, being similar with that in the forest soil, but far below that in the vegetable soil. The bacterial abundance in the tea garden soils was significantly and positively correlated with the soil pH and microbial biomass C (P<0.001) respectively, but significantly and negatively correlated with N application rate and age of tea plantation (P<0.01) respectively. There was no significant correlation between bacterial abundance and total organic C and total N in soil. Multiple regression analysis further indicated that bacterial abundance was affected most significantly by soil pH, followed by age of tea stand and annual N application rate. The results of this study suggested that soil amelioration such as raising soil pH and reducing the high rates of nitrogen application could be of great help for maintaining bacterial abundance and microbial diversity in tea garden soils. Reference | Related Articles | Metrics Cited: Baidu(14) Select Application of Fingerprint Technique in Tea Research LIU Ying, WU Shu-guang, YIN Zhou, WU Guang-hong, CAO Li-fen Journal of Tea Science    2013, 33 (1): 13-20.   DOI: 10.13305/j.cnki.jts.2013.01.009 Abstract （431）      PDF（pc） （593KB）（181）       Knowledge map    Save Chaotic market situation was formed for identifying the quality of tea difficultly, which affected the modern development of tea industry.In recent years, more and more application to sensory evaluation, quality control and geographical origin determination of tea was studied by fingerprinting techniques, which provides effective technical support for the identification of the quality of tea. The methods of fingerprint technique were reviewed in this paper, including fingerprintings based on gas chromatography(GC), liquid chromatography(LC), capillary electrophoresis (CE), infrared （IR）and near-infrared （NIR）spectroscopy, nuclear magnetic resonance (NMR), etc. The problem of the fingerprint in tea applications were analyzed, and the development in tea was predicted in the paper, The article also gives a theoretical basis and reference for further studies of the fingerprint in tea. Reference | Related Articles | Metrics Cited: Baidu(14) Select Fauna of Tea Fulgora and the Major Species in China ZHANG Han-gu Journal of Tea Science    2004, 24 (4): 240-242.   DOI: 10.13305/j.cnki.jts.2004.04.003 Abstract （480）      PDF（pc） （87KB）（266）       Knowledge map    Save There were more than 20 species of fulgora recorded in tea areas of China. They belonged to 6 families of Fulgoroidea. Most of them belonged to Flalidae and Ricaniidae, including several harmful species. Reference | Related Articles | Metrics Cited: Baidu(14) Select Effects of Modifying pH on the Separation of Theaflavins by HSCCC JIANG He-yuan, CHENG Qi-kun, DU Qi-zhen Journal of Tea Science    2003, 23 (s1): 88-91.   DOI: 10.13305/j.cnki.jts.2003.s1.017 Abstract （323）      PDF（pc） （326KB）（128）       Knowledge map    Save The four main kinds of theaflavins could be separated into three parts with the solvent system (Ethyl acetate / Methanol / Hexane / Water, 3/1/1/6) on HSCCC. The different pH zone of elutes could be appeared in the case of the difference of pH between sample solution and mobile phase. Due to the fact of the separation and purification of theaflavins are influenced by the pH of solution, and the process of separation and eluation is closely related to the pH of solution, so the method of modifying pH could be used to highly concentrate the four main kinds of theaflavins from the large scale of samples. Reference | Related Articles | Metrics Cited: Baidu(14) Select Studies on the Spatial Variability and Influencing Factors of Trace Elements in Sichuan Mengshan Montanic Tea Plantation Soils WANG Yong-dong, LIAO Gui-tang, LI Ting-xuan, ZHANG Xi-zhou, HUANG Ping Journal of Tea Science    2008, 28 (1): 14-21.   DOI: 10.13305/j.cnki.jts.2008.01.008 Abstract （275）      PDF（pc） （892KB）（171）       Knowledge map    Save The subtropical hill region is the main area where the tea plantations are distributed in Chian, Trace elements are very important to tea quality, Study on the spatial variability of trace elements in tea plantation soils is the foundation of precision agriculture. With the original data as basis, the spatial variability of trace elements of montanic tea plantation soil in Mengshan is studied with the aid of principal component analysis and geostatistics model. The result showed: (1) The spatial variability of four trace elements(Fe, Mn, Zn, Cu) in tea plantation soils are similar. All of the theoretical model of trace elements are exponential model, the range of spatial correlation is 206~1 860 m. The spatial correlation of Fe, Mn, Zn belong to medium levels [C0/(C0+C): 25.1%~49.6％], the spatial variability between Mn and Cu similar anisotropic characteristic in NE111º, Fe and Zn trends to isotropy. (2) Principal component of comprehensive cartography describe the distribution rule of trace elements in general. Maps of Kriged estimates with principal component showed that the characters of trace elements distributed in a belt shape across the Mengshan mountain, which are in accordance with the result of geostatistic analysis. (3)The mainly influencing factors of tea plantation soil in Mengshan are soil condition and slope. Reference | Related Articles | Metrics Cited: Baidu(14) Select Determination of Theanine in Tea by Paired Ion Chromatography GONG Yu-shun, HUANG Jian-an, CUI Xiang-xing, LI Qing, LI Yin-hua, CHEN Jin-hua Journal of Tea Science    2008, 28 (2): 89-92.   DOI: 10.13305/j.cnki.jts.2008.02.004 Abstract （328）      PDF（pc） （248KB）（184）       Knowledge map    Save A paired ion chromatographic method was developed for rapid determination of theanine in tea. SDS was added as a paired ion reagent, mobile phase was a mixture of acetonitrile and phosphoric acid aqueous solution, detection wavelength was set at 200 nm. The retention time of Theanine was 6.27 min. The calibration of theanine was in good linearity in the range of 0.04~20 μg with the regression equation Y=1.51466×106X+3.28706×105(r2=0.99901). The detection limits was 1.15 ng(S/N=3). This method is rapid, simple, and accurate for the analysis of theanine in tea. Reference | Related Articles | Metrics Cited: Baidu(14) Select Dynamics of Theanine Biosynthesis in Tea Suspension Cells CHENG Hao, GAO Xiu-qing Journal of Tea Science    2004, 24 (2): 115-118.   DOI: 10.13305/j.cnki.jts.2004.02.008 Abstract （309）      PDF（pc） （295KB）（141）       Knowledge map    Save The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30th day and reached a high peak of 20% cell dry weight. Reference | Related Articles | Metrics Cited: Baidu(14) Select Derivation of Theanine and Its Determination by Capillary Electrophoresis LI Ping, WAN Xiao-chun, LI Jian, ZHANG Zheng-zhu, SHEN Zuo-jun Journal of Tea Science    2004, 24 (2): 119-123.   DOI: 10.13305/j.cnki.jts.2004.02.009 Abstract （340）      PDF（pc） （365KB）（209）       Knowledge map    Save A novel derivative method for determination of theanine was developed by micellar electrokinetic capillary chromatography (MECC). Samples were derivated with 2,4-dinitrofluorobenzen. The buffer solution used in this method was 0.03mol/L sodium borate solution (pH9.8). Column temperature was 17℃. The separating voltage was 28βkV and detection at 360βnm. Factors of pH value, reaction time, reaction temperature and concentration of buffer were optimized. The products of derivation of theanine can be stable for five days at 4℃ or 25℃. The linearity of this assay was 0.2~5βmmol/L (r2=0.993). The minimal detection limit (S/N=2) was 0.05βmmol/L. Reference | Related Articles | Metrics Cited: Baidu(14) Select Studies on the Competitive Capacity and Tactics of Chinese Tea on the Overseas Market with SWOT Method JIANG Han-chun, WANG Xiao-gu, ZHAO Hong-ying, JIANG Su-han Journal of Tea Science    2004, 24 (3): 159-165.   DOI: 10.13305/j.cnki.jts.2004.03.003 Abstract （448）      PDF（pc） （46KB）（236）       Knowledge map    Save Tea export plays a key role in Chinese tea economy. Tea has always the significance industrial value in both domestic and international markets. The export advantages and the weaknesses of Chinese tea, the opportunities and threats we are facing were analyzed by comparative principle on economy and SWOT method in this paper. The status and competitive capacity of Chinese tea in international tea markets was enunciated .The green tea and the special kinds of tea possessed competitive advantages must be developed in our country, the green marketing will be practiced, the criteria of domestic tea business will be in accordance with that of overseas ,moreover, the marketing of tea export must be unified. Reference | Related Articles | Metrics Cited: Baidu(14) Select Research Progress on the Health Functions of Tea Polyphenols and Development of Healthy Foods WU Xin-rong, WANG Yue-fei, ZHANG Shi-kang, XU Ping, YANG Xian-qiang Journal of Tea Science    2010, 30 (S1): 501-505.   Abstract （311）      PDF（pc） （182KB）（284）       Knowledge map    Save As a great natural antioxidant, tea polyphenols (TP) and its health function has been one of hot-topic studies at home and abroad. This paper reviewed the new outcomes of epidemiological and clinical studies on tea and health, which including prevention and treatment of cancers, cardiovascular diseases, neurodegenerative diseases and other diseases by TP. Meanwhile the current development and prospects on healthy foods of TP were also mentioned. Reference | Related Articles | Metrics Cited: Baidu(14) Select Construction of Tea Caffeine Synthase Gene RNAi Vector ZHANG Guang-hui, LIANG Yue-rong, LU Jian-liang, DONG Jun-jie Journal of Tea Science    2006, 26 (4): 243-248.   DOI: 10.13305/j.cnki.jts.2006.04.002 Abstract （446）      PDF（pc） （222KB）（322）       Knowledge map    Save Tea Caffeine synthase (TCS) is one of the key enzymes involved in caffieine biosynthsis in tea plant (Camellia sinensis),which catalyses conversions of 7-methylxanthine to theobromine and theobromine to caffeine. Inhibition of TCS gene expression can leads to breeding low caffeine tea cultivars. Two cDNA fragments of TCS gene were amplified by RT-PCR, and ligated into T-vector. The two TCS gene fragments were inserted into RNAi vector pFGC5941 in reverse direction after double digestion with two pairs of restriction endonucleases. The insertion of two fragments, namely pFGC5941-TCS02 and pFGC5941-TCS03, into the RNAi vector were confirmed by PCR and DNA sequencing. Reference | Related Articles | Metrics Cited: Baidu(14) Select Determination of Total Fluoride in Brick Tea with Oxygen Bomb Combustion-Standard and Fluoride Ion Additive Selective Electrode Method WANG Xian-yang, LI Lu-ling Journal of Tea Science    2006, 26 (3): 204-208.   DOI: 10.13305/j.cnki.jts.2006.03.010 Abstract （295）      PDF（pc） （338KB）（188）       Knowledge map    Save The method of determining total fluoride in brick-tea has been investigated, with oxygen bomb combustion-standard and fluoride ion additive selective electrode. The result showed that the sample of brick-tea was burned in the oxygen bomb with 3βMPa purity oxygen, then absorbing the ash with deionization water, the content of total fluoride was determined directly by means of fluoride ion additive selective electrode under pH5.6 and the concentration of sodium citrate dihydrate in TISAB is 110.0βg／L. The average recoveries were 95.88%~101.0% with RSD of 0.75%~1.25%. The fluoride in national tea reference material was determined, and the content of fluoride was accord with the certified values. This method is simple, rapid, accurate． Reference | Related Articles | Metrics Cited: Baidu(13) Select Molecular Cloning and Sequence Analysis of Chalcone Isomerase Gene of Tea Plant (Camellia sinensis) MA Chun-lei, ZHAO Li-ping, ZHANG Ya-li, CHEN Liang Journal of Tea Science    2007, 27 (2): 127-132.   DOI: 10.13305/j.cnki.jts.2007.02.006 Abstract （286）      PDF（pc） （382KB）（185）       Knowledge map    Save Tea is one of the most popular non-alcoholic healthy beverages in the world, which possesses great value as a source of secondary metabolic products, such as catechins. Isolation and cloning of important functional genes of tea plant (Camellia sinensis) is of crucial significance for using biotechnology method to regulate the metabolism of tea plant. In this paper, the chalcone isomerase gene, which was an important functional gene of catechins biosynthesis pathway, was cloned from tea plant by using EST sequencing and RACE (rapid amplification of cDNA ends) approaches. The full-length cDNA of chalcone isomerase gene is 1 163 bp (GenBank Accession No. DQ904329), containing a 723bp open reading frame (ORF) encoding a 240 amino acid protein, and its 3′ untranslated region has an obvious polyadenylation signal. The deduced protein molecular weight was 26.4 kD and its theoretical isoelectric point was 5.19. Sequence analysis result showed that it is closely related with that of Lycopersicon esculentum. Reference | Related Articles | Metrics Cited: Baidu(13) Select Rapid Propagation of Tea Clonal Seedlings in Auto-controlled Greenhouse CHENG Hao, ZENG Jian-ming, ZHOU Jian, WANG Li-yuan, CHANG Jie, GE Ying, YUAN Hai-bo, GU Bao-jing, ZHANG Xiao-fei Journal of Tea Science    2007, 27 (3): 231-235.   DOI: 10.13305/j.cnki.jts.2007.03.009 Abstract （288）      PDF（pc） （408KB）（310）       Knowledge map    Save The rapid propagation of tea clonal seedling was carried out in auto-controlled greenhouse. The result showed that when the technical route of tissue culture-direct rooting-greenhouse incubating was followed, the optimal initial explant was the third axillary bud of spring shoots, which possessed the best germination rate, proliferated 2.75 times in one incubation cycle, and 20% of the cultures could grow to more than 5βcm length every cycles. On the other hand, when the technical route of direct greenhouse cutting and incubation was followed, biannual proliferation could be achieved with one started in middle March and harvested after 5 months while the height of seedlings reached 20.4±8.3βcm, the transplant survival rate of these seedlings was close to 100% after they were proper conditioned. The other proliferation started at about Aug. to Sept., after about 6 month’s incubation, the height of those seedlings reached more than 20βcm after the rootage was established during winter. The increment of seedling height of those fertilized was 5.9 times as much as those unfertilized. Reference | Related Articles | Metrics Cited: Baidu(13) Select Effects of Cadmium Stress on Growth of Tea Plant and Physiological Index in Leaves of Mengshan Tea XIA Jian-guo, LAN Hai-xia Journal of Tea Science    2008, 28 (1): 56-61.   DOI: 10.13305/j.cnki.jts.2008.01.011 Abstract （247）      PDF（pc） （422KB）（137）       Knowledge map    Save Effects of different cadmium concentrations on growth of tea plant and physiological index in leaves of Mengshan tea were studied with the tea cultured in pots. Results showed that, low concentration of cadmium has no obvious adverse effect on the tea plant. When the cadmium concentration reached 60 mg/kg,the tea plant harmed to die; The chlorophyⅡ(ChⅠ) content of most spring tea supplemented with Cd was higher than CK and the increasing trend was raised firstly and inhibited later. The chlorophyⅡ content of summer tea supplemented with Cd was significantly decreased with increase of cadmium concentration, and the chlorophyⅡ content showed a negative relationship with the cadmium concentration, and the coefficient of correlation was -0.997**. The malondialdehyde (MDA) content in spring and summer tea was significantly enhanced with the increase of cadmium concentration. The coefficient of correlation were 0.970**,0.994**（P<0.01）respectively. The free proline of spring tea was significantly enhanced wiyh the increase of Cd concentration, the coefficient of correlation was 0.829*（P<0.05）, the free proline in summer tea was significantly enhanced, and the coefficient of correlation was 0.980**（P<0.01）,and the content of free praline in summer tea was higher than that in spring tea. The soluble sugar content in spring tea supplemented with Cd was higher than CK and the increasing trend raised firstly and then inhibited, the coefficient of correlation was -0.342;The soluble sugar content of summer tea supplemented with Cd was significantly decreased and the coefficient of correlation was -0.909*（P<0.05）. Reference | Related Articles | Metrics Cited: Baidu(13) Select Separation, Purification and Structure Identification of Flavonol Glycosides in Black Tea GAO Qing-qing, JIANG He-yuan, ZHANG Jian-yong, YUAN Xin-yue, KOU Xiao-hong, GAO Qi, LIU Xiao-hui, KE Chang-qiang Journal of Tea Science    2008, 28 (4): 277-281.   DOI: 10.13305/j.cnki.jts.2008.04.003 Abstract （379）      PDF（pc） （455KB）（151）       Knowledge map    Save Column chromatography, HPLC and prepared HPLC were used together to separate and purify the ethyl acetate extract of Dajielee black tea, and the mixture of two flavonol glycosides was obtained. The molecule NMR methods weights and structure of these two flavonol glycosides were identified by using the HPLC/MS and NMR methods. Reference | Related Articles | Metrics Cited: Baidu(13) Select Study on the Content of the Major Mineral Elements in Pu-erh Tea LV Hai-peng, LIN Zhi, ZHANG Yue, LIANG Yue-rong Journal of Tea Science    2013, 33 (5): 411-419.   DOI: 10.13305/j.cnki.jts.2013.05.002 Abstract （300）      PDF（pc） （687KB）（146）       Knowledge map    Save The contents of sixteen mineral elements in Pu-erh tea and sun-dried green tea were determined by ICP-OES, and their contents differences in Pu-erh tea with different grades and in different producing areas were also compared. The contents obtained in mg kg-1 for the elements analyzed were 16.68(B), 3306.64(Ca), 272.18(Fe), 806.05(Al), 14522.80(K), 2054.96(Mg), 682.09(Mn), 0.25(Mo), 487.71(Na), 3628.98(P), 2350.90(S), 173.78(Si), 61.77(Zn), 2.48(Ni), 0.15(Co) and undetectable (Hg). The contents of the Mn, Na, Zn, Ca, Fe, Al, K, Mg, Ni and Co in Pu-erh tea were observed to be much higher those in Sun-dried green tea(P<0.05). Significant differences in the content of Ca, Fe, Al, K, Mg, S, Ni and Co were observed in Pu-erh tea with different producing areas（P<0.05）. However, there were no significant differences in the content of the studied elements except Ni in Pu-erh tea with different grades（P>0.05）. Reference | Related Articles | Metrics Cited: Baidu(13) Select Pb Absorption and Accumulation in Tea Plants HAN Wen-yan, YANG Ya-jun, LIANG Yue-rong, SHI Yuan-zhi, MA Li-feng, RUAN Jian-yun, TANG Jia-yi Journal of Tea Science    2009, 29 (3): 200-206.   DOI: 10.13305/j.cnki.jts.2009.3.004 Abstract （301）      PDF（pc） （210KB）（148）       Knowledge map    Save The characteristics of Pb absorption and accumulation in tea plants were studied by the analysis of Pb concentration in different parts of tea plant, in shoots of various seasons, cultivars and ages of tea plant. The results showed that feeding roots had the highest Pb concentration, followed by bud producing stems, lateral branches and old leaves, tender shoots and tea seeds were the lowest. Pb concentration in shoots and leaves increased with their maturity. Tea mature leaves and shoots had obvious seasonal change in Pb concentration with the highest during April to June and with the lowest in July for mature leaves, and spring > autumn > summer for shoots. A significant correlation was found between shoots and mature leaves in Pb concentration. A tendency was also found that the higher Pb concentration in shoots from the older tea plants. The Pb concentration in shoots from various tea cultivars was different to some degree. Reference | Related Articles | Metrics Cited: Baidu(13) Select Study on Ultrasonic Wave-Assisted Extraction of Tea XIAO Wen-jun, TANG He-ping, GONG Zhi-hua, XIAO Li-zheng, LI Shi, LIU Zhong-hua Journal of Tea Science    2006, 26 (1): 54-58.   DOI: 10.13305/j.cnki.jts.2006.01.009 Abstract （309）      PDF（pc） （324KB）（166）       Knowledge map    Save The extraction effect of tea quality components by the ultrasonic wave-assisted extraction and the traditional water extraction was comparatively studied, and the best technical parameters for ultrasonic wave-assisted extraction was screened out. The results showed that tea extraction by ultrasonic wave-assisted extraction is better than that by traditional water extraction under the same technical parameters condition, which are ratio of tea to water, extraction temperature, time and times. The best operating parameters of tea extraction by ultrasonic wave-assisted extraction are 1:15 ratio of tea to water, 95℃water temperature, two times extraction (1:8+1:7) and 15βmin each time, and the extracted rate of amino acid, water soluble protein, polyphenol, water soluble carbohydrate, caffeine, simple catechin, estered catechin, water extracts can increase 23.13%、36.92%、14.88%、24.40%、19.24%、26.45%、10.12%、12.27% in sequence. Reference | Related Articles | Metrics Cited: Baidu(13) Select Phenotypic Diversity Analysis of Biochemical Composition in 52 Tea Germplasms WANG Xiao-ping, TANG Xiao-bo, WANG Ying-chun, LI Chun-hua, YAO Yu, LI Lan-ying, LUO Fan, WANG Yun Journal of Tea Science    2012, 32 (2): 129-134.   DOI: 10.13305/j.cnki.jts.2012.02.012 Abstract （306）      PDF（pc） （656KB）（151）       Knowledge map    Save Biochemical components (including water extracts, polyphenols, amino acids, caffeine and catechins etc.) and genetic diversity in spring shoots were analyzed in 52 tea germplasms from Sichuan(33 germplasms) and other provinces(19 germplasms). The results showed that the biochemical diversity and variation were significant in these 52 tea gemplasms. Based on the biochemical composition data, 52gemplasms were clustered into two groups. Reference | Related Articles | Metrics Cited: Baidu(13) Select Recognition of Wuyi Zheng Yan Tea with PLS-DA Based on Leverage Correction Using near Infrared Spectra ZHOU Jian, CHENG Hao, WANG Li-yuan, XUE Feng-ren, HE Xiao-yan, XU Jin-bin, HE Wei Journal of Tea Science    2009, 29 (1): 34-40.   DOI: 10.13305/j.cnki.jts.2009.1.006 Abstract （547）      PDF（pc） （320KB）（174）       Knowledge map    Save Wuyi Yan tea was one of Olong tea which was produced only in north of Fujian province. It was a famous tea in China and the quality of Zheng Yan tea which was manufactured of tea leaves planted in the centre area of Wuyi mountain was best in Wuyi Yan tea. To recognize Zheng Yan tea , Partial least Squares Discriminant Analysis（PLS-DA）combined with leverage correction was used to discriminate Zheng Yan tea and Ban yan tea based on near-infrared spectra in this paper. The result showed that Zheng Yan tea could be recognized by PLS-DA method. 100% was correctly recognized in training set(42 samples), test set(20 samples) separately. The result proved the feasibility of discriminant recognition of Zheng Yan tea and Ban Yan tea. Reference | Related Articles | Metrics Cited: Baidu(13) Select Identification, Evaluation and Screening on Elite and Rare Tea Germplasms from Yunnan Province SONG Wei-xi, LIU Ben-ying, YI Bing, JIANG Hui-bing, MA Ling, DUAN Zhi-fen, SUN Xue-mei, WANG Yun-gang, WANG Ping-sheng Journal of Tea Science    2011, 31 (1): 45-52.   DOI: 10.13305/j.cnki.jts.2011.01.008 Abstract （328）      PDF（pc） （196KB）（144）       Knowledge map    Save The agronomic traits, made tea quality, chemical components, cold tolerance and resistance to pests and diseases of 500 accessions tea germplasms collected from Yunnan Province were systematically and comprehensively investigated during 1986~2005. A total of 52 well-performed accessions tea germplasms were screened out according to nearly 100 characteristics. Among which, 32 accessions were relatively coordinated polyphenols, amino acids, caffeine and water extract contents, 2 super early maturity, 5 high polyphenols contents(>38%), 7 high caffeine content(>5.2%), 2 high caffeine content(<1%), 10 high theaflavins content(>1.6%), 26 high water extracts content(>48%), 2 high resistant to root knot nematode, 1 high resistant to tea leafhopper, 2 high resistant to tea red spider mite and 6 strong cold tolerance germplasms. Reference | Related Articles | Metrics Cited: Baidu(13) Select Study and Identification on the Aroma Components of the Representative Products of Yingde Black Tea in Guangdong WANG Qiu-shuang, WU Hua-ling, CHEN Dong, XU Yong-quan, YIN Jun-feng, Huang Guo-zi Journal of Tea Science    2012, 32 (5): 448-456.   DOI: 10.13305/j.cnki.jts.2012.05.002 Abstract （390）      PDF（pc） （691KB）（188）       Knowledge map    Save In the purpose of clarifying the aroma material base different from other black tea in Guangdong Yingde black tea, the determination of main aroma components was conducted, the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds in Yingde congou black tea-9, Yingde congou black tea-1 and Yingde colony black tea produced in Guangdong province, the Yunnan black tea, Indian Assam black tea and Sri Lanka UVA black tea as the control. The result showed that more than 50 aroma compounds were identified including alcohol, ester, aldehyde, ketones, alkene, hydrocarbon and etc., among which the alcohol was the most important compounds accounting for 67.03% and ester and aldehyde were next to it, accounting for 12.85% and 11.33%. Linalool, methl salicylate, linalool oxide, Nerol and nonanal were the five most important compounds, representing more than 60% of the total aroma. Flower-like and sweet aroma compounds were the main aroma character in Guangdong Yingde black tea. Reference | Related Articles | Metrics Cited: Baidu(13) Select The Anti-obesity Effects of Fuzhuan Brick Tea on High-fat-diet Induced Obesity in Rats WANG Die, HUANG Jian-an, YE Xiao-yan, CAO Dan, ZHANG Jian-wei, LIU Zhong-hua Journal of Tea Science    2012, 32 (1): 81-86.   DOI: 10.13305/j.cnki.jts.2012.01.007 Abstract （393）      PDF（pc） （804KB）（163）       Knowledge map    Save The aim of the study was to investigate the anti-obesity effects of the Fuzhuan brick tea and green tea on a high-fat-diet induced rat model. Experimental groups were given daily oral administrations of tea aqueous extracts at different concentrations, which yields doses of 75mg/kg, 150mg/kg or 300mg/kg. The results showed that the body weight gains and the weights of perinephric fat pads and epididymal adipose tissues were significantly reduced in rats administrated with tea aqueous extracts compared with the model control group. Supplementation with tea aqueous extract decreased the values of the food utilization efficiency, fat coefficient and Lee’s index. Histological analysis in the present study showed that both tea aqueous extracts had suppressive effects on the number and size of the adipose tissue fat cells. Moreover, serum levels of the triglycerides of the Fuzhuan brick tea groups are lower than that of the green tea groups. Reference | Related Articles | Metrics Cited: Baidu(12) Select Construction of No-Pollution Control System on Tea Pests Based on Chemical Ecology SUN Xiao-ling, CHEN Zong-mao Journal of Tea Science    2009, 29 (2): 136-143.   DOI: 10.13305/j.cnki.jts.2009.2.009 Abstract （314）      PDF（pc） （270KB）（163）       Knowledge map    Save Progress and application of ecological functions of the sex pheromone of tea pests, herbivore induced tea volatiles, extraneous induction on resistance of tea plant, and behavioral regulation effect of secondary metabolites from nonhost on Ectropis obliqua Prout and Empoasca vitis (Göthe) were introduced. The construction and prospects on no-pollution control system of tea pests based on the current chemical ecology achievements was discussed. Reference | Related Articles | Metrics Cited: Baidu(12) Select Analysis of the Aroma Components in the Pile-Fermentation of Small Leaf Tea Picked in Summer and Autumn JIN Dong-shuang, GONG Shu-ying, LIN Yu-hao, ZHANG Ying-bin, GU Zhi-lei, WANG Hai-jian Journal of Tea Science    2009, 29 (2): 111-119.   DOI: 10.13305/j.cnki.jts.2009.2.005 Abstract （371）      PDF（pc） （329KB）（225）       Knowledge map    Save The pile-fermentation experiment was conducted in Zhejiang province by using the sun-dried raw green tea processed by the summer/autumn small leaf tea plant. The aroma components of the tea sampled in different pile-fermentation time were analyzed by HS-SPME/GC-MS (Headspace-Solid-phase Microextraction/Gas Chromatography-Mass Spectrometry). Resulted showed that eight groups of aroma components were identified, which were terpene and derivatives, hydrocarbons, aromatic hydrocarbons, alcohols, aldehydes, ketones, esters and acids. The main components among them were terpenes and derivatives the contents was up to 70.20% in the finished products. The maximum value of types (totally 47 kinds of compounds) of aroma was on the tenth day and the relative content was maximum (95.78%) on the fifth day after pile-fermentation. Those the components related to the sweet aroma were 3-Octanone, 3-Carene, Selinene and etc, which increasd when the sweet aroma improved. Some new components were detected in the finished products, such as Hexanal, methyl-Cyclopentane, Heptanol, 2-Pentyl-Furan, Isophoron, and others. The characteristic aroma components of the small-leaf pile-fermented tea were includeα-Cedrene, β-Cedrene, α-Gurjunene, β-Cyclocitral,β-Ionone, Cedrol, etc, especially terpene and derivatives. Reference | Related Articles | Metrics Cited: Baidu(12) Select Status of the Total Amount of Rare Earth Oxides in Tea and Its Leaching Characteristics SHI Yuan-zhi, HAN Wen-yan, MA Li-feng, FANG Li Journal of Tea Science    2011, 31 (4): 349-354.   DOI: 10.13305/j.cnki.jts.2011.04.011 Abstract （285）      PDF（pc） （386KB）（156）       Knowledge map    Save In order to figure out the status of total amount of REE oxides（∑REOs） in tea and its leaching characteristics, the statues of ∑REOs in 1245 tea samples from 2007 to 2010 were statistical analyzed, and the leaching characteristics on the different ∑REOs with different processing technology were studied. The results showed that the Median of the ∑REOs in Chinese tea was about 2mg/kg, and the ranges were from ND to 13.80mg/kg, about over 90% of tea were under 5mg/kg. The ∑REOs in over half of Oolong teas were more than 2mg/kg, but for the other tea such as green tea, scented tea, less than 30% of those teas were more than 2mg/kg. When the tea was grinded, the leaching rate of the ∑REOs was increased significantly than the original tea. And the processing technology maybe was the major factor to impact on the leaching rate of the ∑REOs. There were some rules about the leaching rate followed by low to high: needle-shaped tea< flat-shaped tea (such as Longjin tea)< Curl-shaped tea < powdered tea. The ∑REOs was highest in the first brew, and then was decreased along with the 2nd and the 3rd brews. The Powdered Oolong tea has the highest leaching rate about the ∑REOs, the leaching rate was about 60%, but even the ∑REOs in the powdered Oolong tea has reached to 6mg/kg,the ∑REOs in the brew still under the limited value about the standard in drinking water. The needle-shaped tea has the lowest leaching rate about the Reference | Related Articles | Metrics Cited: Baidu(12) Select Research Progress of Cellulase and Pectinase in the Extraction of Tea Leaves GONG Yu-lei, WEI Chun, WANG Zhi-biao, SHEN Xue-liang Journal of Tea Science    2013, 33 (4): 311-321.   DOI: 10.13305/j.cnki.jts.2013.04.004 Abstract （616）      PDF（pc） （1179KB）（701）       Knowledge map    Save Tea is one of the most widely consumed soft beverages. Tea beverages more excellent in flavor and formation of less deposits are more preferred. With the development of modern biotechnology, the microbial enzymes are applied in the extraction process of tea leaves. The cell walls of tea leaf and proteins are easy to be hydrolyzed, and the formation of precipitates can be avoided. The extract yields of polyphenols, thearubigins and amino acids were increased. And the tea qualities including color, odour and flavor improved accordingly. In this article, the hydrolytic enzymes including cellulase, pectinase, tannase and protease are introduced in detail, and their applications in tea extraction process are reviewed. The prospects of complex enzymes system in tea extraction process are also previewed. Reference | Related Articles | Metrics Cited: Baidu(12) Select Study on Antitumor and Immune Regulation Activities of Anjibaicha Polysaccharide XIA Dao-zong, ZHANG Yuan-jun, NI Da-mei, JU Meng-ting Journal of Tea Science    2013, 33 (1): 40-44.   DOI: 10.13305/j.cnki.jts.2013.01.008 Abstract （420）      PDF（pc） （527KB）（166）       Knowledge map    Save The antitumor activity of anjibaicha polysaccharide in vitro by MTT method was investigated by the authors. For the purpose of investigating the antitumor and immune regulation functions of anjibaicha polysaccharide in vivo, to establish the transplant tumor model of mice, and determine the inhibition rate on tumor cells growth and peritoneal macrophage phagocytic function of tumor-burdened mice by anjibaicha polysaccharide. The results showed that the sarcoma-180 growth could be significant inhibited by anjibaicha polysaccharide in vitro and showed dose-dependent relation ship. The test in vivo showed that anjibaicha polysaccharide has significant inhibitory effects on sarcoma-180 and ascitic hepatoma H22 growth in tumor-burdened mice (P<0.05). In addition, anjibaicha polysaccharide could increase thymus and spleen indexs in mice with sarcoma-180 and ascitic hepatoma H22 cells. Moreover, anjibaicha polysaccharide could significantly increase the peritoneal macrophage phagocytic function in sarcoma-180-burdened mice (P<0.05). Reference | Related Articles | Metrics Cited: Baidu(12) Select Recent Advance on the Application of Near-infrared Spectroscopy in Tea ZHOU Jian, CHENG HaoP, WANG Li-yuan Journal of Tea Science    2008, 28 (4): 294-300.   DOI: 10.13305/j.cnki.jts.2008.04.008 Abstract （526）      PDF（pc） （383KB）（326）       Knowledge map    Save The recent advance on the application of Near-infrared spectroscopy in tea was introduced in this paper. Its advantage and disadvantage in comparing with the other analytical methods were also analyzed. The main reasons which limited the development of near-infrared spectroscopic technique was analyzed and the range of the application in tea was predicted in the paper. This article also gives some advices about the development of near-infrared spectroscopy in tea. Reference | Related Articles | Metrics Cited: Baidu(12) Select Studies on the Extraction of Tea-leaf Protein CAI Zhi-ning, WANG Chun-yan, LIANG Cui-jin, LI Qing-yu Journal of Tea Science    2008, 28 (4): 309-312.   DOI: 10.13305/j.cnki.jts.2008.04.001 Abstract （323）      PDF（pc） （278KB）（145）       Knowledge map    Save ：The investigation was designed by using these reference factor including the solid to liquid ratio, extraction time, pH value and extraction temperature, as to determine the optimum technological parameters of tea-leaf protein extraction process and the optimum combination. Results showed that the optimum condition is as follows: solid to liquid ratio of 1:8, pH11, 60℃, 60 min. The protein extraction rate was 21.89% under these conditions. The extraction process of tea protein and the equipment used are simple. The optimal pH of sedimentation is pH 4.0. Reference | Related Articles | Metrics Cited: Baidu(12) Select Studies on the Effect of Traditional Baking Process on Quality of Lingtou Dancong Oolong Tea WANG Deng-liang, GUO Qin, ZHANG Da-chun Journal of Tea Science    2004, 24 (3): 197-200.   DOI: 10.13305/j.cnki.jts.2004.03.009 Abstract （346）      PDF（pc） （35KB）（174）       Knowledge map    Save The changes in the contents of the related bio-chemical components and the aroma constituents between final-firing sample and baking sample were analyzed. Results showed that the contents of the water soluble matters、polyphenols、amino acid、soluble sugar、TF and TR were decreased, and the contents of the TB were increased; concentration of aromatic constituents that showed aroma of flowers was decreased, and the concentration of aromatic constituents that showed aroma of honey was increased. That is to say that the baking process showed its key role in the formation of high aroma and mellow sensory tasting in Lingtou Dancong Oolong Tea. Reference | Related Articles | Metrics Cited: Baidu(12) Select The Main Active Component of Lipophilic Tea Polyphenols and in vitro Inhibition Activity on Ovarian Cancer Cells HO-8910 CHEN Ping，ZHONG Jian-hua，SUN Dong Journal of Tea Science    2003, 23 (2): 115-118.   DOI: 10.13305/j.cnki.jts.2003.02.006 Abstract （257）      PDF（pc） （353KB）（117）       Knowledge map    Save A single-substitution long-chain lipophilic catechin was isolated and purified from lipophilic tea polyphenols by high-speed countercurrent chromatography (HSCCC). Its molecular structure was elucidated as epigallocatechin-3-O-gallate-4'-palmitate by elemental analysis, IR, MS and 1H-NMR. The in vitro inhibition activity of the lipid-soluble epigallocatechin-3-O-gallate-4'-palmitate on ovarian cancer cells HO-8910 was investigated and compared with that of green tea polyphenols and lipophilic tea polyphenols. The results demonstrated that the inhibition activity of the single-substitution EGCG palmitate was almost corresponding to green tea polyphenols, and more effective than lipophilic tea polyphenols. Reference | Related Articles | Metrics Cited: Baidu(12) Select Selection of Early Budding and High Quality Green-tea Cultivar YANG Ya-jun, YANG Su-juan, WANG Yu-shu, ZENG Jian-ming Journal of Tea Science    2003, 23 (s1): 9-15.   DOI: 10.13305/j.cnki.jts.2003.s1.002 Abstract （336）      PDF（pc） （311KB）（176）       Knowledge map    Save ：The comparison test and the reginal experiment of 6 new clones including Zhongcha 108 (RadiationII) and 4 cultivars including Zhongcha 102 were carried out. The results of the comparison test showed that Zhongcha 108 was ultra-early budding clone. The content of amino acid of Zhongcha 108 was as high as 4.53% , it was suitable for the manufacture of green-tea and the quality of made-tea was excellent. Its yield was 67.23% higher than that of Fuding Dabaicha (CK). The result of regional experiment indicated that Zhongcha102 was early budding cultivar, the content of amino acid was 4.46%, it was suitable for the manufacture of green-tea and the quality of tea was excellent, the yield was 21.68% higher than that of Yingshuang (CK). The growth rate of Zhongcha 102 is vigorous and with strong cold resistance. Reference | Related Articles | Metrics Cited: Baidu(12) Select Review on Antioxidative Mechanism of Theaflavins CHEN Hu, HU Yin, ZHOU Rui, ZHAO Jian Journal of Tea Science    2005, 25 (4): 237-241.   DOI: 10.13305/j.cnki.jts.2005.04.001 Abstract （371）      PDF（pc） （247KB）（173）       Knowledge map    Save The content of theaflavins is an important index of black tea’s quality, are the major substance in black tea, which show significant antioxidative effect. The antioxidative mechanism of theaflavins was revieved in this paper Reference | Related Articles | Metrics Cited: Baidu(12) Select Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant HONG Yong-cong, XIN Wei, LAI Yu-bin, WENG Xin, HU Fang-ping Journal of Tea Science    2005, 25 (3): 183-188.   DOI: 10.13305/j.cnki.jts.2005.03.005 Abstract （388）      PDF（pc） （352KB）（160）       Knowledge map    Save The screening and identification of endophytic bacteria from the healthy and diseased leaves of various tea cultivar plants were conducted. Results showed that the endophytic bacteria were accounted in 2.9×106~39.4×106βcfu/(g•fw) in tea cultivars plants. The TL2 stain showed strongly antagonistic activity against leaf fungal pathogens of tea-plant. It showed a good protecting effect when inoculated the TL2 strain bacteria firstly than inoculated the tea grey blight pathogen(Pestallozzia theae) later, Besides, it showed a strong degrative activity to cypermethrin. The TL2 bacteria strain could colonize within tea plant. According to the results of identification, the TL2 bacteria strain was identified as bacillas subtilis. Reference | Related Articles | Metrics Cited: Baidu(12) Select Study on the Method of Determining Tea Color with Computer WANG Wen-jie, LUO Shou-jin, HUANG Jian-qin, ZHANG Li-ping, WU Xin-rong, WANG Hong-shu Journal of Tea Science    2005, 25 (1): 37-42.   DOI: 10.13305/j.cnki.jts.2005.01.007 Abstract （282）      PDF（pc） （277KB）（169）       Knowledge map    Save Reference | Related Articles | Metrics Cited: Baidu(11) Select Genetic Variation in the Leaf-Expansion Period of the First Hybrid Generation Tea Plants GUO Ji-chun, YE Nai-xing, HE Xiao-yan Journal of Tea Science    2004, 24 (4): 255-259.   DOI: 10.13305/j.cnki.jts.2004.04.006 Abstract （275）      PDF（pc） （195KB）（162）       Knowledge map    Save This research adopted the Huangjingui, Huangqi, Huangguanyin, Tieguanyin and Hongyafoshou as parent plants to make artificial hybridization between cultivars with various sprouting date and to observe the trend of genetic variation in the F1 leaf-expansion period. Results showed that:① The F1 leaf-expansion periods occurred a successive variation with the average value of their parent leaf-expansion periods as the central value, the variation range was large and the average value of the F1 leaf-expansion period was close to the average value of their parents. ② In the combinations of early-sprouting and early-sprouting cultivars, the super-parental phenomenon in both positive and negative directions of genetic variation of shortening or postponing the F1 leaf-expansion period were observed. In the combination of late-sprouting and early-sprouting cultivars, the F1 leaf-expansion periods were located between their parents. In the combination of late-sprouting and middle-sprouting cultivars, the F1 leaf-expansion period were mostly close to the that of middle-sprouting cultivars. ③ The male paternal showed more influence on the leaf-expansion period in F1 generation. When the leaf-expansion period of male parent was earlier, the F1 leaf-expansion period was also early in F1 generation. If the leaf-expansion period of paternal plant were same, the leaf-expansion period in F1 generation was related to the difference of the leaf-expansion period between their parents. When the difference between their parental plants was small, the large variation of leaf-expansion period occurred in the F1 generation. For these reasons, it is possible to acquire the hybrid with the earlier leaf-expansion period than their parental plants via through the hybridization between early-sprouting and early-sprouting cultivars. Simultaneously, it is possible to forecast the F1 average leaf-expansion period in the F1 generation and possible variation range in the F1 generation according to the paternal leaf-expansion period, the average value and their difference of leaf-expansion periods between the two parents. Reference | Related Articles | Metrics Cited: Baidu(11) Select Analysis of Thermal Efficiency during the Processing of Microwave De-enzyming of the Fresh Tea Leaves YE Yang, YUAN Hai-bo, YIN Jun-feng, LIN Zhi Journal of Tea Science    2004, 24 (4): 270-275.   DOI: 10.13305/j.cnki.jts.2004.04.009 Abstract （278）      PDF（pc） （43KB）（149）       Knowledge map    Save It has not been reported the specific heat of fresh tea leaves that measured by the method of “mixing”. When the leaves contain 50% to 72% of the moisure the average heat ratio of the leaves is 3.44KJ/kg·℃. The results of the experiemnts showed that the main factor that affect the efficiency of heat is the quantity of the leaves during microwave de-enzyming. While the efficiency related with the thickness of the layer of tea leaves and methods for treatment need to be further study. The thermal efficiency of microwave de-enzyming is 32.32% to 38.62%, higher than that of machinery microwave processing, which is 21.23% and 18.11%. The experimental data may be useful to improvement of the efficiency of machinery microwave processing which is used in tea processing factory. Reference | Related Articles | Metrics Cited: Baidu(11) Select Studies on the Green Fluorescence of Tea Polyphenols ZHANG Li-xia, JIA Ming, XIA Wen-juan, XIANG Qin-zeng, HUANG Xiao-qin Journal of Tea Science    2004, 24 (4): 288-294.   DOI: 10.13305/j.cnki.jts.2004.04.012 Abstract （432）      PDF（pc） （659KB）（165）       Knowledge map    Save The effects of the concentration , pH, solvent, mental ion ( Ca2+ , Al3+ ) and heating on the fluorescence spectra of the tea polyphenols were studied. The results showed that the factors of all above had obviously effects on the fluorescent intensity of tea polyphenols. It reached the maximum intensity when pH of solvent is 7.4±0.2 or the concentration of tea polyphenols is 3βmg/ml or water solution contains 50% ethanol. Adding Ca2+ and Al3+ in the infusion of tea polyphenols reduced the fluorescent intensity, but it was mainly indirect effect by precipitating a portion of tea polyphenols. Heating treatment caused the fluorescent intensity of tea polyphenols higher. .The maximum emission peak would move to long wavelength when the concentration of tea polyphenols was above 3βmg/ml or pH>8, but other factors had less effects. Besides, solvent polarity affected the shape of excitation fluorescent spectra and the intensity obviously, but had less effect on the maximum emission wavelength. Reference | Related Articles | Metrics Cited: Baidu(11) Select Improvement of Magnesium Nutrition for Tea Gardens on Hilly Red Soil in the Southern Hunan Province ZHU Yong-xin, CHEN Fu-xing Journal of Tea Science    2003, 23 (s1): 34-37.   DOI: 10.13305/j.cnki.jts.2003.s1.007 Abstract （272）      PDF（pc） （278KB）（154）       Knowledge map    Save Magnesium content in the soils and tea leaves of hilly red soil tea gardens in the southern Hunan area were determined. Results showed that the contents of available magnesium in the soils ranged in 6.29—25.98βmg/kg, with average of 13.02βmg/kg, and the content of available magnesium in tea leaves with symptom of magnesium-deficient is 264.10βmg/kg. The magnesium-deficient symptom was disappeared after applying water-soluble magnesium sulphate, and tea yield was raised for 9.8%—13.6%. By using foliar-applying magnesium fertilizer, the average increase of tea yield reached 20.8%. Applying magnesium fertilizers can also improve tea quality notablely. Compared with the control, the contents of amino acids, caffeine and water extracts were increased for 5.8%, 9.2% and 2.3% respectively. The paper also discussed the situation of magnesium nutrition of tea gardens in hilly red soil area of the southern China, and the magnesium fertilizer developing strategy was suggested as well. Reference | Related Articles | Metrics Cited: Baidu(11) Select Determination of Trace Lead in Tea with Screen-printed Carbon Electrodes ZHAO Guang-ying, WU Yan-yan Journal of Tea Science    2008, 28 (2): 93-100.   DOI: 10.13305/j.cnki.jts.2008.02.005 Abstract （297）      PDF（pc） （537KB）（138）       Knowledge map    Save The square wave anodic stripping voltammetric (SWASV) methodology using a screen printed carbon electrode (SPCE) in situ plated in mercury (Ⅱ) was developed by the authors to determine lead in tea. A detailed description of the systematic laboratory test carried out to optimize its performance is reported. A series of optimum parameter are as follows Hg2+concentration: 4×10-4 mol/L, Plating potential: -1.1 V, Estep: 3 mV, Frequency: 10 Hz, Amplitude: 60 mV, pH: 5, plating time: 280 s, balance time: 30 s. Under the optimized analytical condition, the obtained sensitivity, linearity, and detection limit are 22.7 nA/(μg/L), 10~225 μg/L (r=0.9986), and 0.74 μg/L(S/N=3),respectively. The interference effect was thoroughly studied with various metals in the determination of 120 μg/L Pb2+. Tea was digested using domestic microwave oven and PTFE sealed and pressurized microwave digestion tank. The standard method of analysis was used to detect the samples and comprised with the GB method, no significant deviation was found. The method was sensitive, accurate, fast and suitable for the determination of trace lead in tea. Reference | Related Articles | Metrics Cited: Baidu(11) Select Requirement on Nitrogen, Phosphorus and Potassium by Tea Plants with Different Ages under Field Conditions YOU Xue-qin, YANG Ya-jun, RUAN Jian-yun Journal of Tea Science    2008, 28 (3): 207-213.   DOI: 10.13305/j.cnki.jts.2008.03.007 Abstract （276）      PDF（pc） （280KB）（121）       Knowledge map    Save In order to study on requirement on nitrogen, phosphorus and potassium by tea plants with different ages under field conditions, the biomass on up-ground parts of Longjing 43 tea plants aged 1, 2, 3, 5 and 10 years in the fall of the first year, and the young shoots, the fallen leaves and the pruning on the second year under different N, P and K application was investigated. The biomass of up-ground parts in tea plants aged 5 years and the concentration of N, P and K in tea plants increased 32, 21, 25, 29 times respectively as compared with those aged one year. The biomass and the concentration of N, P and K in tea plants aged 10 years only increased approximately by 71%, 17%, 13%, 14% respectively as compared with those aged 5 years. The annual requirement of N, P and K for tea plants aged 1~2 years under field conditions is 90.01, 5.71, 31.11 kg/hm2 respectively. The annual requirement of N, P and K for tea plants aged 2~3 years is 122.74, 9.63, 45.14 kg/hm2 respectively. The annual requirement of N, P and K for tea plants aged 3~4 years is 144.03, 10.33, 47.99 kg/hm2 respectively. After pruning, the absorbed N, P and K in part is recovered to the soil through pruning and fallen leaves, the amounts of N, P and K in t