XU Fan, XIA Xiaojiao, LI Jingjing, CAO Yuxi, LI Hengbiao, LIN Ling, XU Wei
Using one bud and one or two leaves of ‘Fuding Dabaicha' as raw materials, this study compared the effects of room temperature natural fermentation and dynamic temperature-controlled fermentation on the quality of summer Sichuan Congou black tea. A comprehensive evaluation was conducted using sensory evaluation, routine physicochemical analysis, UPLC-MS/MS non-targeted metabolomics and HS-SPME-GC-MS volatile metabolomics. Sensory evaluation results indicate that the variable temperature group (KW group) exhibited better sensory quality than the room temperature group (SW group), characterized by a more mellow and fresh taste, a distinct sweet aroma, and longer aroma persistence. Physicochemical analysis indicates that compared with the SW group, the KW group had increased contents of water extract, soluble sugars, theaflavins, and thearubigins, while the contents of caffeine, total catechins, and theabrownins were decreased. A total of 1 950 metabolites were identified using UPLC-MS/MS, among which 170 differential metabolites were screened (44 upregulated and 126 downregulated). KEGG enrichment analysis reveals that pathways such as phosphatidylinositol signaling system, flavone and flavonol biosynthesis, flavonoid biosynthesis, and anthocyanin biosynthesis were significantly enriched, suggesting that dynamic temperature-controlled fermentation may affect the taste and color quality of tea infusion by regulating these pathways. A total of 938 volatile metabolites were identified using HS-SPME-GC-MS, and the total amount of volatile compounds in the KW group was higher than that in the SW group. Relative odor activity value (rOAV) analysis identifies 169 key aroma compounds in the two tea samples. Compounds representing fruity and fresh aroma attributes, such as 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone, (E)-4-nonenal, (E)-2-hexenal, and (E,Z)-2,6-nonadienal, were higher in the KW group than in the SW group. In conclusion, Sichuan Congou black tea processed by dynamic temperature-controlled fermentation exhibited better quality than that processed by room temperature natural fermentation. These findings provided a theoretical basis for improving the quality of summer Sichuan Congou black tea and for the high-value utilization of summer fresh tea leaves.