Genetic Diversity Analysis of Euproctis pseudoconspersa and Its Bunyavirus (EpBYV) in China

CHEN Shichun; JIANG Hongyan; LIAO Shuran; CHEN Tingxu; NIU Jinzhi; WANG Xiaoqing

doi:10.13305/j.cnki.jts.2024.05.009

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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (5) : 793-806. DOI: 10.13305/j.cnki.jts.2024.05.009

Research Paper

Genetic Diversity Analysis of Euproctis pseudoconspersa and Its Bunyavirus (EpBYV) in China

CHEN Shichun¹, JIANG Hongyan¹, LIAO Shuran¹, CHEN Tingxu¹, NIU Jinzhi², WANG Xiaoqing^1,*

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Abstract

Tea tussock moth, Euproctis pseudoconspersa, is an important pest which damages tea plants and causes human dermatitis. Euproctis pseudoconspersa bunyavirus, EpBYV, is a bunyavirus that widely distributed in different geographical populations of E. pseudoconspersa. In order to control the E. pseudoconspersa and utilize the virus resources, it is necessary to fully understand the genetic background of E. pseudoconspersa and EpBYV. In this study, 148 samples of E. pseudoconspersa larvae from 15 geographic populations were collected. COI and ND5 gene sequences of E. pseudoconspersa and RdRp sequences of EpBYV were determined. The genetic diversities of E. pseudoconspersa and EpBYV were analyzed by DnaSP 6.12.03, Arlequin 3.5.2.2 and MEGA 7.0.26. Sequence analysis of the combined sequences of COI and ND5 genes shows that 15 geographic populations have high haplotype diversity (h=0.880 68) and low nucleotide diversity (π=0.003 17). Significantly high genetic differentiation among 99 population pairs (F_ST>0.290, P<0.05) was identified. Molecular variance analysis (AMOVA) shows that the genetic differentiation of E. pseudoconspersa was mainly among populations (87.12%), and the differentiation among groups was consistent with the second and third ladder boundaries in China. Demographic history analysis suggests that the population of E. pseudoconspersa is relatively stable. RdRp sequences were successfully amplified in the 138 samples except CK population. RdRp sequence analysis reveals that the 14 geographic populations of EpBYV had high haplotype diversity (h=0.935 26) and relatively low nucleotide diversity (π=0.017 95). The 93 population pairs had significantly higher genetic differentiation (F_ST>0.257, P<0.05). AMOVA analysis shows that the genetic differentiation of EpBYV was mainly between populations (62.13%). Demographic history analysis reveals that EpBYV might have undergone population expansions in the past. Based on the analysis of this study, the population of E. pseudoconspersa in China is relatively stable, and there is a risk of population expansion in Chengkou, Chongqing and Ningde, Fujian. The population of EpBYV has experienced population expansion. The infection rate and population expansion ability of EpBYV in E. pseudoconspersa are high, which has good potential for biological control of E. pseudoconspersa.

Key words

Euproctis pseudoconspersa / bunyavirus / EpBYV / genetic diversity / tea plant

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CHEN Shichun, JIANG Hongyan, LIAO Shuran, CHEN Tingxu, NIU Jinzhi, WANG Xiaoqing. Genetic Diversity Analysis of Euproctis pseudoconspersa and Its Bunyavirus (EpBYV) in China[J]. Journal of Tea Science. 2024, 44(5): 793-806 https://doi.org/10.13305/j.cnki.jts.2024.05.009

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